Enhanced Production of a Human Spumavirus (Retroviridae) in Semi-permissive Cell Cultures after Treatment with 5-Azacytidine

Hotta, Jun‐ichi; Loh, Philip C.

doi:10.1099/0022-1317-68-4-1183

Cited by 9 publications

(3 citation statements)

References 16 publications

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“…It is known that epithelial cells do not support active replication of foamy virus (15); yet, it remains unclear if the resistance of epithelial cells is due to a lack of specific receptors or to the absence of intracellular factors. Several latently infected cultures have been established from epithelial cells (6,11,22) and lymphoblastoid cells (20); failure of HFV replication in these cultures has been suggested to be caused by methylation of proviral DNA (11,22). In these cases, very little or no infectious virus was produced and only proviral DNA was detected.…”

mentioning

confidence: 99%

Productive persistent infection of hematopoietic cells by human foamy virus

Stone

Linial

1996

J Virol

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Human foamy virus can establish persistent infections in human hematopoietic cell lines, such as H92.1.7 (erythroblastoid cells), Jurkat (CD4 ؉ T cells), and U937 (myeloid-monocytic cells). The infection is characterized by constant production of infectious viruses (for >2 1/2 years) with no cytopathic effects on the host cells. Electron microscopy of the infected cells showed a viral morphology similar to that observed for particles produced after acute infection. We have detected, in addition to the full-length form of bel1, a previously described deletion in the bel1 gene of the proviral DNA in these cells. RNA containing this 301-bp deletion, which mapped to the splice donor and acceptor sites of the intron of the bet gene, was also found in encapsidated virion RNA. However, the presence of this defective provirus harboring the deletion in bel1 does not prevent productive persistence in these chronically infected cells, since the virus titer does not decrease during cultivation.

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mentioning

confidence: 99%

Productive persistent infection of hematopoietic cells by human foamy virus

Stone

Linial

1996

J Virol

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“…HFV has been shown to replicate well in fibroblasts and poorly in epithelial cells (7). Further, HFV infection in primary and cultured human leukocytes was demonstrated very recently (11).…”

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confidence: 99%

Replication of Feline Syncytial Virus in Feline T‐Lymphoblastoid Cells and Induction of Apoptosis in the Cells

Ikeda

Itagaki

Tsutsui

et al. 1997

Microbiology and Immunology

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Feline syncytial virus (FSV) was isolated from feline peripheral blood mononuclear cells of FSV-seropositive cats. When the susceptibility of feline T-lymphocytes to FSV was examined using three strains of FSV, FSV antigens were detected in the FSV-infected T-lymphoblastoid cells. Further, a diversity of biological properties, including replication kinetics and syncytia formation, was noted among the strains, and condensation of chromatin and the fragmentation of cellular DNA were observed in the infected cells. From these data, we conclude that FSV is lymphotropic and can induce apoptosis in the lymphocytes.

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“…Transcription of SFVmcy_FV21 and SFVcae_LK3 (previously designated as SFV-1 and SFV-3) has been shown to be cell-line dependent [39] and productive, persistent infection of hematopoietic cells by SFV psc_huHRSV.13 (previously designated as HFV) has been reported [37]. Latently infected cultures with SFV have been established from epithelial cells [40][41][42] and lymphoblastoid cells [43]. Furthermore, in vivo SFV infection is widespread throughout the animal but remains generally latent except in the oral tissues [44].…”

Section: Discussionmentioning

confidence: 99%

Genome Analysis and Replication Studies of the African Green Monkey Simian Foamy Virus Serotype 3 Strain FV2014

Fuentes

Bae

Nandakumar

et al. 2020

Viruses

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African green monkey (AGM) spumaretroviruses have been less well-studied than other simian foamy viruses (SFVs). We report the biological and genomic characterization of SFVcae_FV2014, which was the first foamy virus isolated from an African green monkey (AGM) and was found to be serotype 3. Infectivity studies in various cell lines from different species (mouse, dog, rhesus monkey, AGM, and human) indicated that like other SFVs, SFVcae_FV2014 had broad species and cell tropism, and in vitro cell culture infection resulted in cytopathic effect (CPE). In Mus dunni (a wild mouse fibroblast cell line), MDCK (Madin-Darby canine kidney cell line), FRhK-4 (a fetal rhesus kidney cell line), and MRC-5 (a human fetal lung cell line), SFVcae_FV2014 infection was productive resulting in CPE, and had delayed or similar replication kinetics compared with SFVmcy_FV21 and SFVmcy_FV34[RF], which are two Taiwanese macaque isolates, designated as serotypes 1 and 2, respectively. However, in Vero (AGM kidney cell line) and A549 (a human lung carcinoma cell line), the replication kinetics of SFVcae_FV2014 and the SFVmcy viruses were discordant: In Vero, SFVcae_FV2014 showed rapid replication kinetics and extensive CPE, and a persistent infection was seen in A549, with delayed, low CPE, which did not progress even upon extended culture (day 55). Nucleotide sequence analysis of the assembled SFVcae_FV2014 genome, obtained by high-throughput sequencing, indicated an overall 80–90% nucleotide sequence identity with SFVcae_LK3, the only available full-length genome sequence of an AGM SFV, and was distinct phylogenetically from other AGM spumaretroviruses, corroborating previous results based on analysis of partial env sequences. Our study confirmed that SFVcae_FV2014 and SFVcae_LK3 are genetically distinct AGM foamy virus (FV) isolates. Furthermore, comparative infectivity studies of SFVcae_FV2014 and SFVmcy isolates showed that although SFVs have a wide host range and cell tropism, regulation of virus replication is complex and depends on the virus strain and cell-specific factors.

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Enhanced Production of a Human Spumavirus (Retroviridae) in Semi-permissive Cell Cultures after Treatment with 5-Azacytidine

Cited by 9 publications

References 16 publications

Productive persistent infection of hematopoietic cells by human foamy virus

Productive persistent infection of hematopoietic cells by human foamy virus

Replication of Feline Syncytial Virus in Feline T‐Lymphoblastoid Cells and Induction of Apoptosis in the Cells

Genome Analysis and Replication Studies of the African Green Monkey Simian Foamy Virus Serotype 3 Strain FV2014

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