Enhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium <i>Geobacillus icigianus</i> BITSNS038

Soy, Snehi; Nigam, Vinod Kumar; Sharma, S.

doi:10.1080/16583655.2021.2002549

Cited by 9 publications

(8 citation statements)

References 69 publications

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“…showed promise as a candidate for fast growth and amylase enzyme production within 24 h [31,33,34]. Soy et al [33] found that Geobacillus icigianus BITSNS038 produced maximum amylase enzyme production within 24 h using 10 g/L of starch as a carbon source and inducer, while Ulya et al [31] also reported amylase enzyme production by Geobacillus sp. JSI within 24 h of cultivation.…”

Section: Discussionmentioning

confidence: 99%

Raw starch degrading alkaline α‐amylase from Geobacillus kaustophilus TSCCA02: Production, characterization, and its potential for application as a detergent additive

Phonlamai,

Kingkaew,

Prajanket

et al. 2024

J Basic Microbiol

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Geobacillus kaustophilus TSCCA02, a newly isolated strain from cassava (Manihot esculenta L.) rhizosphere soil in Thailand, showed maximum raw starch degrading enzyme (RSDE) activity at 252.3 ± 9.32 U/mL with cassava starch and peptone at 5.0 and 3.0 g/L, respectively. 16 S ribosomal RNA (rRNA) sequencing and phylogenetic tree analyses indicated that the TSCCA02 strain was closely related to G. kaustophilus. The crude RSDE had optimal activity at 60°C and pH 9.0. This enzyme degraded various kinds of starch including potato starch, cassava starch, rice flour, corn starch, glutinous rice flour, and wheat flour to produce sugar syrup at 60°C, as confirmed by scanning electron microscopy (SEM), thin‐layer chromatography (TLC), and Fourier‐transform infrared spectroscopy (FTIR). The major end products of starch hydrolysis were maltose and maltotriose with a small amount of glucose, confirming this enzyme as an α‐amylase. The enzyme improved the washing efficiency of cotton fabric with commercial detergent. Results indicated the potential of alkaline α‐amylase produced from a new isolate of G. kaustophilus TSCCA02 for application as a detergent additive on an industrial scale.

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Section: Discussionmentioning

confidence: 99%

Raw starch degrading alkaline α‐amylase from Geobacillus kaustophilus TSCCA02: Production, characterization, and its potential for application as a detergent additive

Phonlamai,

Kingkaew,

Prajanket

et al. 2024

J Basic Microbiol

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“…Previous study documented that partially purified enzyme from B. licheniformis HULUB1 and B. subtilis SUNGB2 has played a vital role in degradation of waste food material at 65 °C [ 49 ]. Soy et al [ 15 ] reported about application of crude amylase from Geobacillus icigianus . Enzyme was highly efficient in starch hydrolysis and showed 34.5% hydrolysis of corn starch slurry.…”

Section: Discussionmentioning

confidence: 99%

“…The release of reducing sugar was recorded at 450 nm by using the spectrophotometer (Model: AE-S90-MD, UK). Glucose was utilized as calibration standard (1–15 μg ml-1) [ 14 , 15 ].…”

Section: Methodsmentioning

confidence: 99%

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Optimization of amylase production using response surface methodology from newly isolated thermophilic bacteria

Sharif

Shah

Fariq

et al. 2023

Heliyon

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“…The amylase was partially puri ed having molecular weight between 45-63 kDa [33]. Partially puri ed amylase characterization was done using UPLC, DLS-ZP, and TGA.…”

Section: Characterization Of Partially Puri Ed Amylasementioning

confidence: 99%

Metagenomic analysis of Hot Springs of Surajkund and the exploration of thermostable enzymes from endogenous isolates

Soy

Lakra

Prakash

et al. 2023

Preprint

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Hot springs serve as a hub for microbial diversity, the microflora of the hot springs can be a source of numerous biologically important molecules. Extremophile bacteria were isolated from different hot springs of India to investigate their potential in industrial applications. The analysis involved both culture dependent and culture independent methods. The metagenomic analysis of the microflora was carried out using next-gen sequencing. Also, thermophilic isolates were culture and primarily screened for amylase, xylanase and cellulase enzyme production. The production for amylase was optimized and partial purification was carried out. Partially purified amylase was tested and characterized using; UPLC, DLS-ZP and TGA. The retention time for the amylase was observed to be around 0.5 minutes along with a stable thermo gravimetric profile around 400oC confirming its stability at higher temperatures. One of the thermophilic isolate was able to degrade lignocellulosic waste exhibiting the potential of isolates from the kund.

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Enhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium Geobacillus icigianus BITSNS038

Cited by 9 publications

References 69 publications

Raw starch degrading alkaline α‐amylase from Geobacillus kaustophilus TSCCA02: Production, characterization, and its potential for application as a detergent additive

Raw starch degrading alkaline α‐amylase from Geobacillus kaustophilus TSCCA02: Production, characterization, and its potential for application as a detergent additive

Optimization of amylase production using response surface methodology from newly isolated thermophilic bacteria

Metagenomic analysis of Hot Springs of Surajkund and the exploration of thermostable enzymes from endogenous isolates

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