Enhanced polyhydroxybutyrate (PHB) accumulation in heterotrophically grown Arthrospira platensis under nitrogen deprivation

Duangsri, Chanchanok; Mudtham, Nat-Anong; Incharoensakdi, Aran; Raksajit, Wuttinun

doi:10.1007/s10811-020-02272-4

Cited by 12 publications

(10 citation statements)

References 56 publications

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“…The characteristic wave numbers of PHB in the FTIR spectrum were presented by the strong carbonyl group (C=O) at 1726 cm −1 and asymmetric C-O-C stretching vibration at 1279 cm −1 , which are typical for ester bondings in PHB polyesters (Figure 4). The patterns are consistent with earlier reports [54]. Other adsorption bands obtained at 1460 and 1378 cm −1 designated the -CH 2 and -CH 3 groups, respectively.…”

Section: Characterisation Of Extracted Polyestersupporting

confidence: 92%

Leptolyngbya sp. NIVA-CYA 255, a Promising Candidate for Poly(3-hydroxybutyrate) Production under Mixotrophic Deficiency Conditions

2022

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Cyanobacteria are a promising source for the sustainable production of biodegradable bioplastics such as poly(3-hydroxybutyrate) (PHB). The auto-phototrophic biomass formation is based on light and CO2, which is an advantage compared to heterotrophic PHB-producing systems. So far, only a handful of cyanobacterial species suitable for the high-yield synthesis of PHB have been reported. In the present study, the PHB formation, biomass, and elemental composition of Leptolyngbya sp. NIVA-CYA 255 were investigated. Therefore, a three-stage cultivation process was applied, consisting of a growth stage; an N-, P-, and NP-depleted phototrophic stage; and a subsequent mixotrophic deficiency stage, initiated by sodium acetate supplementation. The extracted cyanobacterial PHB was confirmed by FTIR- and GC-MS analyses. Furthermore, the fluorescent dyes LipidGreen2 and Nile red were used for fluorescence-based monitoring and the visualization of PHB. LipidGreen2 was well suited for PHB quantification, while the application of Nile red was limited by fluorescence emission crosstalk with phycocyanin. The highest PHB yields were detected in NP- (325 mg g−1) and N-deficiency (213 mg g−1). The glycogen pool was reduced in all cultures during mixotrophy, while lipid composition was not affected. The highest glycogen yield was formed under N-deficiency (217 mg g−1). Due to the high carbon storage capacity and PHB formation, Leptolyngbya sp. NIVA-CYA 255 is a promising candidate for PHB production. Further work will focus on upscaling to a technical scale and monitoring the formation by LipidGreen2-based fluorometry.

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Section: Characterisation Of Extracted Polyestersupporting

confidence: 92%

Leptolyngbya sp. NIVA-CYA 255, a Promising Candidate for Poly(3-hydroxybutyrate) Production under Mixotrophic Deficiency Conditions

2022

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“…33 PHB showed characteristic peaks at 2974 and 2931 cm À1 (C H stretching), 1720 cm À1 (ester carbonyl group C=O), 1454 cm À1 (C H asymmetric deformation), 1379 cm À1 (H C O in plan bending), 1275 cm À1 (C O C backbone asymmetric stretching vibration), 1227 cm À1 (C C O asymmetric stretching), 1180 cm À1 (C C O backbone methylene wagging), and 1130 and 1100 cm À1 (C O C stretching vibration). [34][35][36][37][38] The PHB composites containing ZnO particles presented a similar spectrum to pristine PHB, as observed in Figure 3(B), associated with its major chemical composition, i.e., polymeric matrix. As highlighted in Figure 3(C) and (D), few spectral changes are related to ZnO presence and interaction with the polymeric structure.…”

Section: Fourier-transform Infrared Spectroscopysupporting

confidence: 55%

A promising sustainable PHB‐ZnO composite for development of biodegradable filaments

et al. 2021

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The development of new biodegradable composites with superior properties is an attractive approach to develop environmentally friendly materials with new potential applications. In this work, we prepared and characterized PHB filaments with different concentrations of ZnO particles (from 0.5 up to 16 wt%) to evaluate this mixture's effect on the physico-chemical, thermal properties, and morphological biofilament characteristics. The FTIR and Raman results indicated a possible chemical interaction between the ZnO and PHB, altering the helical polymeric and crystalline structure. The ZnO particles increased the composites' thermal stability, mainly at higher filler contents, confirmed

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“…Commonly, poly-3-hydroxybutyrate (PHB) is one of the PHAs that can be extracted from microbial biomasses. The highest accumulation of PHB in Arthrospira platensis was observed in cells grown photoautotrophically under nitrogen deprivation with acetate supplementation [13]. PHB is an attractive alternative to common thermoplastics due to its hydrophobicity, biodegradability, and biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

“…The second reaction is the conversion of acetoacetyl-CoA to 3-hydroxybutyryl-CoA by acetoacetyl-CoA reductase (encoded by phaB). Finally, the 3-hydroxybutyryl-CoA monomers are polymerized into poly-3-hydroxybutyrate (PHB) by PHA synthase (encoded by phaC) [13].…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of poly-3-hydroxybutyrate and carotenoids by Arthrospira platensis under combined glycerol and phosphorus supplementation

Satchasataporn¹,

Duangsri²,

Charunchaipipat³

et al. 2022

ScienceAsia

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Arthrospira platensis is one of the most beneficial cyanobacteria due to its high protein content and highvalue compounds such as poly-3-hydroxybutyrate (PHB) and carotenoids which can be used in promoting health in many sectors. A. platensis cultivation can be performed using urban or industrial wastewater as a source of nutrients, including glycerol and phosphorus. The objective of this study was to enhance biomass, pigments, and partial PHB in A. platensis IFRPD1182 grown under different glycerol and phosphorus concentrations. The highest values of biomass, carotenoids, and PHB production were observed in cells grown in the G 3 P 3 medium supplemented with 4.6 g/l glycerol and 356 mg/l phosphorus on the fifth day of cultivation with maximal values of 1.90 ± 0.03 mg/ml, 6.30 ± 0.12 mg/l, and 34.76 ± 2.71 mg/l, respectively. In addition, RT-PCR analysis revealed that the cells grown in G 3 P 3 medium increased crtB and crtP transcripts, encoding phytoene synthase and phytoene desaturase, respectively, and involved in carotenoid biosynthesis with 1.47-and 1.50-fold increases, respectively. This indicates that the increased expression of crtB and crtP genes in the cells can be achieved by a combination of glycerol and phosphorus, where the carotenoids were highly accumulated.

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Enhanced polyhydroxybutyrate (PHB) accumulation in heterotrophically grown Arthrospira platensis under nitrogen deprivation

Cited by 12 publications

References 56 publications

Leptolyngbya sp. NIVA-CYA 255, a Promising Candidate for Poly(3-hydroxybutyrate) Production under Mixotrophic Deficiency Conditions

Leptolyngbya sp. NIVA-CYA 255, a Promising Candidate for Poly(3-hydroxybutyrate) Production under Mixotrophic Deficiency Conditions

A promising sustainable PHB‐ZnO composite for development of biodegradable filaments

Enhanced production of poly-3-hydroxybutyrate and carotenoids by Arthrospira platensis under combined glycerol and phosphorus supplementation

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