Arthrospira platensis is one of the most beneficial cyanobacteria due to its high protein content and highvalue compounds such as poly-3-hydroxybutyrate (PHB) and carotenoids which can be used in promoting health in many sectors. A. platensis cultivation can be performed using urban or industrial wastewater as a source of nutrients, including glycerol and phosphorus. The objective of this study was to enhance biomass, pigments, and partial PHB in A. platensis IFRPD1182 grown under different glycerol and phosphorus concentrations. The highest values of biomass, carotenoids, and PHB production were observed in cells grown in the G 3 P 3 medium supplemented with 4.6 g/l glycerol and 356 mg/l phosphorus on the fifth day of cultivation with maximal values of 1.90 ± 0.03 mg/ml, 6.30 ± 0.12 mg/l, and 34.76 ± 2.71 mg/l, respectively. In addition, RT-PCR analysis revealed that the cells grown in G 3 P 3 medium increased crtB and crtP transcripts, encoding phytoene synthase and phytoene desaturase, respectively, and involved in carotenoid biosynthesis with 1.47-and 1.50-fold increases, respectively. This indicates that the increased expression of crtB and crtP genes in the cells can be achieved by a combination of glycerol and phosphorus, where the carotenoids were highly accumulated.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.