Enhanced photodynamic therapy of mixed phase TiO2(B)/anatase nanofibers for killing of HeLa cells

Abstract

Photodynamic therapy (PDT), which is a procedure that uses photosensitizing drug to apply therapy selectively to target sites, has been proven to be a safe treatment for cancers and conditions that may develop into cancers. Nano-sized TiO 2 has been regarded as potential photosensitizer for UV light driven PDT. In this study, four types of TiO 2 nanofibers were prepared from proton tri-titanate (H 2 T 3 O 7 ) nanofiber. The as-obtained nanofibers were demonstrated as efficient photosensitizers for PDT killing of HeLa cells. MTT assay and flow cytometry (FCM) were carried out to evaluate the biocompatibility, percentage of apoptotic cells, and cell viability. The non-cytotoxicity of the as-prepared TiO 2 nanofibers in the absence of UV irradiation has also been demonstrated. Under UV light irradiation, the TiO 2 nanofibers, particularly the mixed phase nanofibers, displayed much higher cell-killing efficiency than Pirarubicin (THP), which is a common drug to induce the apoptosis of HeLa cells. We ascribe the high cellkilling efficiency of the mixed phase nanofibers to the bandgap edge match and stable interface between TiO 2 (B) and anatase phases in a single nanofiber, which can inhibit the recombination of the photogenerated electrons and holes. This promotes the charge separation and transfer processes and can produce more reactive oxygen species (ROS) that are responsible for the killing of HeLa cells.

Tuning and understanding the phase interface of TiO2 nanoparticles for more efficient lithium ion storage

“…In our previous work, [19][20][21][22] based on the phase transformation invariant line strain (ILS) theory and highresolution transmission electron microscopy (HRTEM) investigation, we have determined that (001) plane of anatase phase has the same orientation with (100) plane of TiO 2 (B) phase, hence the (010) plane of anatase can extend across into the adjacent TiO 2 (B) phase. The determined interphase interface provides a very good opportunity to observe the novel interfacial Li storage behaviour in mixed phase TiO 2 anode materials.…”

Synthesis and Characterization of Tio2 Nanoparticles Using Cynodon Dactylon Leaf Extract for Antibacterial and Anticancer (A549 Cell Lines) Activity

“…A549 lung carcinoma non small cell lines is an adenoma lung cancer cell lines, which is widely used to study the investigation of cytotoxicity, when nanoparticles induce the cell line [6]. Recently TiO 2 nanoparticles used for the several types of cancers are MCF 7 cell lines [7], A549 cell lines [8], HeLa cell lines [9]. TiO 2 nanoparticles induce in the cell lines, and then ROS (Reactive Oxygen species) is produced.…”

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“…The serotonergic system modulates mood [2], anxiety [3], aggression [4], reward systems [5], and impulsivity [6], and psychosis [7]. Many psychiatric diseases, such as depression, obsessive-compulsive disorder, and bulimia nervosa are currently treated by medications targeting the serotonergic system [8,9]. Abnormalities in the serotonergic system have also been implicated in diseases affecting early development such as sudden infant death syndrome [10] and autism [11].…”