Enhanced oxidase/peroxidase-like activities of aptamer conjugated MoS₂/PtCu nanocomposites and their biosensing application

Abstract

Taking advantage of bimetallic alloy nanoparticles and MoS2 nanosheets, a colorimetric aptasensor was developed for MUC1 overexpressed cancer cell detection.

Two-Dimensional Nanomaterials With Enzyme-Like Properties for Biomedical Applications

“…With a substrate of either TMB or H 2 O 2 , the bimetallic nanocomposites gave both lower K m values and larger V max values ( Table 1) than those of monometallic ones, indicating better affinity and POD-like activity by introducing bimetallic NPs into g-C 3 N 4 NSs. Similar observations were obtained by Pt/CuO (Wang X. H. et al, 2017), IrO 2 /rGO (Liu X. L. et al, 2019), Pd/NiCl 2 (Cai et al, 2018a), and PtM/MoS 2 (M = Ag, Cu, and Au) (Cai et al, 2016(Cai et al, , 2017Qi et al, 2016) as reported by our group.…”

“…For example, Yang's group found that the few-layer MoS 2 NSs prepared by liquid-exfoliation exhibited ternary activities (POD-, OD-, and CAT-like activities) (Chen T. M. et al, 2018). As discussed above, these enzymatic activities could be closely associated with specific reaction conditions especially system pH, in which the acidic conditions are usually favorable for POD/OD-mimic catalysis (Qi et al, 2016;Cai et al, 2017;Darabdhara et al, 2019;Feng et al, 2020), while a high pH is beneficial to CAT-catalysis (Liu Y. et al, 2014;Ruan et al, 2018;Wang et al, 2019). Due to the use of various 2D NMs in biomedical fields, for a meaningful discussion of enzymelike activity, the reaction parameters, nature of materials (e.g., morphology, structure, composition, and size), capping agents, and surface charge, are believed to be taken into account .…”

“…The multifunctional enzymatic properties of hybridized 2D NMs provide valuable opportunities to develop advanced biosensors. By taking advantage of POD-/OD-like activity of 2D NMs, the colorimetric assays for other analytes like AA (Wang X. H. et al, 2017;Sun et al, 2020), biothiols [e.g., GSH (Liu et al, 2017;Ge et al, 2019;Liu X. L. et al, 2019), cysteine (Liu X. L. et al, 2019), and homocysteine (Liu X. L. et al, 2019)], xanthine (Cai et al, 2018b;Wu et al, 2018;Li et al, 2019), dopamine (Dutta et al, 2015;Ivanova et al, 2019), cholesterol (Zhang et al, 2017), acetylcholinesterase (Yan et al, 2017), cancer cells (Tao et al, 2013;Zhang L. N. et al, 2014;Qi et al, 2016), ions [e.g., S 2− (Liu et al, 2020), Fe 2+ (Wang et al, 2016), and Pb 2+ (Tang et al, 2020)], have also been established by different groups. The colorimetric biosensing based on 2D nanozymes continues to be a rapidly growing field.…”

The age of bioinspired molybdenum‐involved nanozymes: Synthesis, catalytic mechanisms, and biomedical applications

“…[112][113][114] A novel MoS 2 /PtCu nanocomposite with excellent OXD-like activity has been constructed for detection of cancer cells. 74 The nanocomposites quickly catalyzed the oxidation of three common chromogenic substrates such as TMB, ABTS, and OPD in the presence of dissolved oxygen in solution, producing typical colors. On the basis of OXD-like activity of MoS 2 /PtCu and mucin 1 (MUC1) aptamer, selective binding of aptamer-conjugated MoS 2 /PtCu aptasensor (apt-MoS 2 /PtCu) on the MUC1overexpressed cells converted the recognition process into a quantitative colorimetric signal.…”

Doping and Decorating 2D Materials for Biosensing: Benefits and Drawbacks

“…A novel bimetallic platinum–copper nanoparticle‐decorated MoS 2 composite was synthesized by Qi et al. [ 239 ] for the colorimetric detection of cancerous mucin 1 (MUC1) overexpressed cells. The colorimetric biosensor was fabricated by functionalizing the composite with MUC1 aptamers.…”