Enhanced Mid-Infrared Chemical Imaging (IRCI) Detection of DNA Microarrays

Schoen, Brianna; Mossoba, Magdi M.; Chizhikov, Vladimir; Rashid, A.; Martinez-Diaz, Karen; Al-Khaldi, Sufian F.

doi:10.1366/12-06772

Cited by 1 publication

(3 citation statements)

References 12 publications

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“…In this proof-of-concept study, we have applied a microarray assay with vibrational spectroscopic detection methods 22 previously used to identify bacterial species in our laboratory 23,24 to demonstrate that this methodology can also be successfully used to identify catfish species. Oligonucleotide probes were carefully designed and tested in silico and then printed in triplicate arrays to a slide (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Each spectral image was obtained from approximately a 180 × 180 μm 2 test sample area with a nominal spatial resolution of 5.6 μm per pixel and a spectral resolution of 32 cm −1 . For each image, a single scan was collected and required approximately 1 s. 24…”

Section: Methodsmentioning

confidence: 99%

“…This IRCI detection method was successfully applied to the microarray-based genomic analysis of microorganisms in our laboratory. 23,24…”

Section: Introductionmentioning

confidence: 99%

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Microarray Chip Development Using Infrared Imaging for the Identification of Catfish Species

et al. 2014

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Several families of catfish species are extensively aquacultured around the world; however, only those from the family Ictaluridae can be labeled as catfish in the United States. Non-Ictalurid catfish species that are marketed as "catfish" in the USA are considered misbranded. Misbranding in general has led to an increased interest in developing deoxyribonucleic acid (DNA)-based methods such as DNA barcoding, polymerase chain reaction restriction fragment length polymorphism, and DNA microarrays with fluorescence detection for the identification of fish species. In this proof-of-concept study, DNA microarrays coupled with a newly developed mid-infrared imaging detection method were applied to the identification of seven species of catfish for the first time. Species-specific DNA probes targeting three regions per species of the cytochrome c oxidase 1 (barcoding) gene were developed and printed as microarrays on glass slides. Deoxyribonucleic acid targets labeled with biotin were hybridized to their complementary probes using a strategy that allowed the selective formation of a silver layer on hybridized spots needed for detection. Using this three-probe format, the seven species were all identified correctly, even when a limited number of false positive spots were observed. Raman spectroscopy was employed to further characterize the arrays.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%