Microarray Chip Development Using Infrared Imaging for the Identification of Catfish Species

Abstract

Several families of catfish species are extensively aquacultured around the world; however, only those from the family Ictaluridae can be labeled as catfish in the United States. Non-Ictalurid catfish species that are marketed as "catfish" in the USA are considered misbranded. Misbranding in general has led to an increased interest in developing deoxyribonucleic acid (DNA)-based methods such as DNA barcoding, polymerase chain reaction restriction fragment length polymorphism, and DNA microarrays with fluorescence detection for the identification of fish species. In this proof-of-concept study, DNA microarrays coupled with a newly developed mid-infrared imaging detection method were applied to the identification of seven species of catfish for the first time. Species-specific DNA probes targeting three regions per species of the cytochrome c oxidase 1 (barcoding) gene were developed and printed as microarrays on glass slides. Deoxyribonucleic acid targets labeled with biotin were hybridized to their complementary probes using a strategy that allowed the selective formation of a silver layer on hybridized spots needed for detection. Using this three-probe format, the seven species were all identified correctly, even when a limited number of false positive spots were observed. Raman spectroscopy was employed to further characterize the arrays.

Review: Progress and potential of DNA barcoding for species identification of fish species

“…These advanced methods of DNA barcoding have been applied in unknown species identification, point mutations, biodiversity assessment and food security (Trivedi et al 2016). Handy et al (2014) identified reported misidentification of seven catfish species in USA seafood market by using DNA microarrays with newly developed mid-infrared imaging detection method.…”

Utilizing Big Data to Identify Tiny Toxic Components: Digitalis

“…The world of eukaryotic DNA-based species identification in food and dietary supplements products has undergone a revolution in the past 10 years, and while progress has been swift it has not been without challenges [8][9][10]. Contemporary methods of DNAbased identification include DNA fingerprinting techniques like restriction fragment length polymorphisms [11], microarrays [12], a full suite of polymerase chain reaction (PCR) methods, [13][14][15][16][17], in addition to traditional DNA barcoding [9,18] and a variety of next generation sequencing (NGS) approaches [19][20][21].…”