Enhanced microbial biomass assay using mutant luciferase resistant to benzalkonium chloride

Anticancer Activity of Flavone of Excoecaria agallocha in Cytotoxicity Studies Using Hela Cells

“…Different methods used are measurement of radioisotope adding as a measure of DNA synthesis, counting by automated counters and others which rely on dyes and cellular activity. The MTT (3-[4,5-dimethylthiazo-2-yl]-2,5-diphenyl tetrazolium bromide) system is a means of grading the activity of living cells via mitochondrial dehydrogenases [11][12][13][14][15]. The MTT method is simple, accurate and yields reproducible results.…”

Anti-microbial Action against VerotoxigenicEscherichia coliO157:H7 of Nitric Oxide Derived from Sodium Nitrite

“…ATP content was obtained with the method described by Hattori. 15) Chemicals. Polymyxin B was purchased from Denka Seiken (Tokyo, Japan) and erythromycin, streptomycin and ampicillin were obtained from Sigma (St. Louis, MO, USA).…”

The Terrestrial Bioluminescent Animals of Japan

“…We point out that these luminous animals and their luminescence have been, in general, very poorly investigated: for most of them, only taxonomic description and basic biological notes are available. The only exception is the "Genji-botaru" firefly L. cruciata (Ohba, 1988): its vision (Gleadall et al, 1989;Hariyama et al, 1998;Oba and Kainuma, 2009) and morphological and behavioral variations (Ohba, 2001) have been intensively studied; its luciferin and luciferases, including their structural interaction by X-ray crystallography (Nakatsu et al, 2006) have been identified, and utilized, together with L. lateralis luciferase, for such purposes as sensitive microbial detection and scientific education (Nakano, 1991;Hattori et al, 2003;Murakami et al, 2004); ecological information has been extensively compiled for conservation, educational, and eco-tourism applications (Yajima, 1978;Ohba, 1988Ohba, , 2004cOhba, , 2006Ohba, , 2009Tokyo Fireflies Ecology Institute, 2004); and protocols for rearing it have been established (Ohba, 1988;Tokyo Fireflies Ecology Institute, 2004). There is no doubt that L. cruciata serves as the model organism for the study of bioluminescence and promises to further contribute to our understanding of bioluminescence.…”