Several of these compounds showed antibacterial activities when tested against gram-positive and gram-negative species. One compound i.e. 4b showed promising activities across both the species.
In this study, the compound flavone of mangrove plant Excoecaria agallocha was subjected to cytotoxicity studies using Hela cells was produced from ATCC, stock cells was cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10 % inactivated fetal bovine serum (FBS), penicillin (100 IU/mL), streptomycin (100 µg/mL) in an humidified atmosphere of 5 % CO2 at 37 ºC until confluent. The cell was dissociated with trypsin phosphate versene glucose (TPVG) solution (0.2 % trypsin, 0.02% EDTA, 0.05 % glucose in PBS). The viability of the cells are checked and centrifuged. Further 50,000 cells/well was seeded in a 96 well plate and incubated for 24 h. At 37 ºC, 5 % CO2 incubator, it was observed that while concentration increases, the inhibition increases. Finally, the compound showing good activity in Hela cells. Docetaxel was used as standard drug.
The bio-materials of four marine mangrove medicinal plants viz., Aegiceras Corniculatum (AGC), Excoecariaagallocha (EA), Rhizophoramucronata (RM) and Xylocarpusgranatum (XG) are extracted with methanol and hexane. These extracts are submitted to the antibacterial activity towards the strains
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