Enhanced Mg²⁺‐ATPase activity in ghosts from HS erythrocytes and in normal ghosts stripped of membrane skeletal proteins may reflect enhanced aminophospholipid translocase activity

Abstract

Hereditary spherocytosis (HS) is a congenital haemolytic anaemia which is characterized by a great variety of structural defects in the red cell's membrane skeleton and/or deficiencies in particular membrane (skeletal) proteins. Enhanced (Mg2+)-dependent adenosine triphosphatase (Mg(2+)-ATPase) activities, varying from 115% to 160%, were invariably found in erythrocyte ghosts derived from 13 HS patients. Similarly, an enhancement of Mg(2+)-ATPase activity by 30% is observed in normal red cell ghosts that have been stripped of the greater part of their membrane skeletal proteins by treatment with a low ionic strength buffer. Reassociation of those stripped ghosts with spectrin reduces the enhanced Mg(2+)-ATPase activity to its original level. Since in both cases, HS ghosts and stripped normal ghosts, the stabilizing effects that the membrane skeleton exerts on the maintenance of an endofacial localization of the aminophospholipids are impaired, the enhanced Mg(2+)-ATPase activity is interpreted to reflect an increased activity of the aminophospholipid translocase. The present observations therefore support a role of the membrane skeleton in the stabilization of phospholipid asymmetry in the red cell membrane and consequently in reducing the energy consumption of the translocase.

Erythrocyte membrane integrity and protein activity in high-fat diet fed male Wistar rats

“…ATPase [44,45]. The results from this study show a slight increase in the activity of Ca 2+ Mg 2+ ATPase in the HFD treatment group, which suggest likely increases in erythrocyte intracellular Ca 2+ levels in this group.…”

Preventive Effects of (–) Epicatechin on Altered Adenosine Triphosphatases and Minerals in Isoproterenol‐Induced Myocardial Infarcted Rats

“…This can result in disturbances in Ca 2+ signaling, ventricular performance, and contractility. Magnesium‐dependent adenosine triphosphatase (Mg 2+ ATPase) is believed to be responsible for the aminophospholipid translocase activity of the plasma membrane , which could affect the activity of membrane calcium dependent adenosine triphosphatase (Ca 2+ ATPase) .…”

Catecholamine toxicity triggers myocardial membrane destabilization in rats: thymol and its counter action

“…Intracellular Ca 2+ overload can set off a cascade of events that can lead to the formation of reactive oxygen species, which suggests that reactive oxygen species formation and calcium surge may be involved in the contractile dysfunction of the ischemic myocardium. 37 Mg 2+ -ATPase is believed to be responsible for the aminophospholipid translocase activity of the plasma membrane, 38 which could affect the activity of membrane Ca 2+ -ATPase. 39 Also, in a previous report, a decrease in the adenosine triphosphate (ATP) content was observed in ISO induced cardiotoxic rats.…”