Catecholamine toxicity triggers myocardial membrane destabilization in rats: thymol and its counter action

Meeran, Mohamed Fizur Nagoor; Jagadeesh, Govindan Sangaran; Selvaraj, Palanisamy

doi:10.1039/c5ra00903k

Cited by 13 publications

(5 citation statements)

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“…Diminished concentrations of plasma GSH, an intracellular free-radical scavenger, is believed to be due to its counteractivity against free radicals in oxidative stress triggered by ISO [19]. Moreover, diminished levels of vitamin C and E are attributed to their increased utilization against reactive oxygen species (ROS) [20]. The increase in heart weight occurs due to a rise in edematous intramuscular space following cardiomyocyte injury and swelling, along with the invasion of the injured tissues by defensive inflammatory cells.…”

Section: Discussionmentioning

confidence: 99%

“…In an in vitro assay, the reducing power of NKT was estimated using a previously described method of Oyaizu [53]. Varying volumes of NKT (10,20,30,40, and 50 µM) were mixed with 2.5 mL of phosphate buffer (0.2 M, pH 6.6) and 2.5 mL of potassium ferricyanide (1% w/v). The reaction mixture was incubated at 50 • C for 20 min.…”

Section: In Vitro Reducing Power Of Nktmentioning

confidence: 99%

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Nootkatone, a Dietary Fragrant Bioactive Compound, Attenuates Dyslipidemia and Intramyocardial Lipid Accumulation and Favorably Alters Lipid Metabolism in a Rat Model of Myocardial Injury: An In Vivo and In Vitro Study

et al. 2020

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In the present study, we assessed whether nootkatone (NKT), a sesquiterpene in edible plants, can provide protection against dyslipidemia, intramyocardial lipid accumulation, and altered lipid metabolism in a rat model of myocardial infarction (MI) induced by subcutaneous injections of isoproterenol (ISO, 85 mg/kg) on days 9 and 10. The rats were pre- and co-treated with NKT (10 mg/kg, p.o.) administered daily for 11 days. A significant reduction in the activities of myocardial creatine kinase and lactate dehydrogenase, as well as non-enzymatic antioxidants, and alterations in lipids and lipoproteins, along with a rise in plasma lipid peroxidation and intramyocardial lipid accumulation, were observed in ISO-treated rats. ISO administration induced alterations in the activities of enzymes/expressions that played a significant role in altering lipid metabolism. However, NKT treatment favorably modulated all biochemical and molecular parameters altered by ISO and showed protective effects against oxidative stress, dyslipidemia, and altered lipid metabolism, attributed to its free-radical-scavenging and antihyperlipidemic activities in rats with ISO-induced MI. Additionally, NKT decreased the accumulation of lipids in the myocardium as evidenced from Oil red O staining. Furthermore, the in vitro observations demonstrate the potent antioxidant property of NKT. The present study findings are suggestive of the protective effects of NKT on dyslipidemia and the underlying mechanisms. Based on our findings, it can be suggested that NKT or plants rich in NKT can be promising for use as a phytopharmaceutical or nutraceutical in protecting the heart and correcting lipid abnormalities and dyslipidemia, which are risk factors for ischemic heart diseases.

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Reducing Power Of Nktmentioning

confidence: 99%

Nootkatone, a Dietary Fragrant Bioactive Compound, Attenuates Dyslipidemia and Intramyocardial Lipid Accumulation and Favorably Alters Lipid Metabolism in a Rat Model of Myocardial Injury: An In Vivo and In Vitro Study

et al. 2020

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“…As a counter mechanism against ROS, cells are packed with nonenzymatic antioxidants, such as glutathione and vitamin‐C. Many experimental, clinical, and epidemiologic studies have revealed that therapeutic intervention with antioxidants may interrupt the injury cascade and protect the myocardium at risk …”

Section: Introductionmentioning

confidence: 99%

“…The auto‐oxidation of catecholamines triggers the generation of highly cytotoxic free radicals that stimulates lipid peroxidation and causes irreversible damage to the myocardium . Thus, ISO‐induced MI is used as a common and well standardized experimental in vivo model to study the impact of many drug‐measuring cardiac function and biochemical changes with an extraordinary technical simplicity, low mortality, and reproducibility . The necrotic lesions developed via free radical–mediated oxidative stress after supramaximal doses of ISO also resemble MI in humans…”

Section: Introductionmentioning

confidence: 99%

Protective effects of α‐bisabolol on altered hemodynamics, lipid peroxidation, and nonenzymatic antioxidants in isoproterenol‐induced myocardial infarction: In vivo and in vitro evidences

Meeran

Laham

Al-Taee

et al. 2018

J Biochem & Molecular Tox

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The effect of α-bisabolol on hemodyanimcs, lipid peroxidation, and nonenzymatic antioxidants was evaluated in isoproterenol-induced myocardial infarction in rats. They were pre- and cotreated with α-bisabolol (25 mg/kg body weight) daily for 10 days along with the subcutaneous injection of isoproterenol (85 mg/kg body weight) at an interval of 24 hours for 2 days (9th and 10th days). Increased activities of serum creatine kinase and creatine kinase-MB along with altered levels/concentrations of lipid peroxidation products and nonenzymatic status were observed in the plasma and heart tissues of rats. Treatment with α-bisabolol showed protective effects by reversing the altered biochemical parameters and hemodynamics studied. The in vitro reducing power of α-bisabolol confirmed its potent antioxidant action. These biochemical benefits were translated into functional recovery by the maintenance of the hemodynamics in rats. The findings showed that α-bisabolol has the potential to protect against isoproterenol-induced myocardial infarction due to its potent antilipid peroxidation and antioxidant properties.

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“…Troponin elevation occurs through six pathobiological mechanisms: myocyte necrosis, apoptosis, normal myocyte turnover, cellular release of proteolytic troponin degradation products, increased cell wall permeability, and formation and release of membranous blebs 21) . Specifically, myocardial ischemia may induce apoptosis 22) , transient increases in cardiomyocyte permeability 23) , membranous blebs that rupture 24) , free radical overload 25) , increased troponin turnover 26) , and direct toxic effects of catecholamines 27) .…”

Section: Resultsmentioning

confidence: 99%

Serum High-Sensitivity Cardiac Troponin T as an Independent Predictor for Incident Coronary Heart Disease in the Japanese General Population: The Circulatory Risk in Communities Study (CIRCS)

Imano

Yamagishi

Ohira

et al. 2023

JAT

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Aims: Epidemiological evidence is extremely limited about high-sensitivity cardiac troponin T (hs-cTnT) and future coronary heart disease (CHD) events for the general population in countries with low mortality from CHD. Therefore, we investigated the association between hs-cTnT levels and the risk of incident CHD using a nested case-control study in a large Japanese cohort study. Methods:The participants were residents of four Japanese communities in the Circulatory Risk in Communities Study (CIRCS). We obtained 120 cases (81 men and 39 women, aged 38-86 years at baseline) of first incident CHD and 240 controls matched by age, sex, communities, and blood sampling term. Serum hs-cTnT levels were measured using an electrochemiluminescence immunoassay with stored sera collected between 2001 and 2011. The median period between sampling at survey and CHD incidence was 2.0 (interquartile range, 0.9-3.7) years. After adjusting for conventional risk factors, the multivariable odds ratios (ORs) of CHD were calculated using conditional logistic regression analyses.Results: hs-cTnT ranged from ≤ 3 (assay detection limit) to 155 ng/L. Compared with the lowest quartile of hs-cTnT, multivariable ORs (95% confidence intervals) of CHD for the second, third, and highest quartiles were 1.30 (0.57-2.95), 2.48 (1.09-5.64), and 3.01 (1.27-7.12), respectively. Similar associations were observed after adjusting for estimated glomerular filtration, or after excluding matched groups, including people with chronic kidney disease. Conclusion:Serum hs-cTnT could predict CHD in the Japanese general population. These findings implicate a benefit from monitoring hs-cTnT to predict CHD even among populations in countries with low mortality from CHD.

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Catecholamine toxicity triggers myocardial membrane destabilization in rats: thymol and its counter action

Abstract: The present manuscript deals with the protective action of thymol against isoproterenol induced cardiotoxicity by reinstating ATPases and minerals as evidenced by decreased myocardial infarct size.

Cited by 13 publications

References 40 publications

Nootkatone, a Dietary Fragrant Bioactive Compound, Attenuates Dyslipidemia and Intramyocardial Lipid Accumulation and Favorably Alters Lipid Metabolism in a Rat Model of Myocardial Injury: An In Vivo and In Vitro Study

Nootkatone, a Dietary Fragrant Bioactive Compound, Attenuates Dyslipidemia and Intramyocardial Lipid Accumulation and Favorably Alters Lipid Metabolism in a Rat Model of Myocardial Injury: An In Vivo and In Vitro Study

Protective effects of α‐bisabolol on altered hemodynamics, lipid peroxidation, and nonenzymatic antioxidants in isoproterenol‐induced myocardial infarction: In vivo and in vitro evidences

Serum High-Sensitivity Cardiac Troponin T as an Independent Predictor for Incident Coronary Heart Disease in the Japanese General Population: The Circulatory Risk in Communities Study (CIRCS)

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