Enhanced mannanase production by submerged culture of Aspergillus niger NCH-189 using defatted copra based media

Lin, Tse-Chun; Chen, Chinshuh

doi:10.1016/s0032-9592(03)00218-8

Cited by 46 publications

(39 citation statements)

References 34 publications

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“…The results of this study were consistent with the findings obtained by Lin and Chen [14], who reported that peak b-mannanase activity was attained when A. niger was grown on b-mannanase inducer carbon sources at 30°C. In contrast, Kote et al [12] have shown that the cultivation of A. niger gr at 40°C and A. flavus gr at 35°C in medium containing defatted copra leads to the production of maximum levels of b-mannanase in culture.…”

Section: Effect Of Incubation Temperaturesupporting

confidence: 92%

“…Although several studies have been carried out on the use of hemicellulosic compounds in the production of b-mannanase via SmF process [1,14,28,29], few attempts have been made to produce b-mannanase using agro-industrial carbon sources in the SSF process [13]. On the other hand, most studies on b-mannanase production by fungi under SSF have focused on the production of enzyme in shake flasks, and there has been much less work on the use of bioreactors for the production of b-mannanase in SSF [18].…”

Section: Introductionmentioning

confidence: 99%

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Utilization of palm kernel cake for production of β-mannanase by Aspergillus niger FTCC 5003 in solid substrate fermentation using an aerated column bioreactor

Abdeshahian

Samat

Hamid

et al. 2009

J Ind Microbiol Biotechnol

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The production of beta-mannanase from palm kernel cake (PKC) as a substrate in solid substrate fermentation (SSF) was studied using a laboratory column bioreactor. The simultaneous effects of three independent variables, namely incubation temperature, initial moisture content of substrate and airflow rate, on beta-mannanase production were evaluated by response surface methodology (RSM) on the basis of a central composite face-centered (CCF) design. Eighteen trials were conducted in which Aspergillus niger FTCC 5003 was cultivated on PKC in an aerated column bioreactor for seven days under SSF process. The highest level of beta-mannanase (2117.89 U/g) was obtained when SSF process was performed at incubation temperature, initial moisture level and aeration rate of 32.5 degrees C, 60% and 0.5 l/min, respectively. Statistical analysis revealed that the quadratic terms of incubation temperature and initial moisture content had significant effects on the production of beta-mannanase (P < 0.01). A similar analysis also demonstrated that the linear effect of initial moisture level and an interaction effect between the initial moisture content and aeration rate significantly influenced the production of beta-mannanase (P < 0.01). The statistical model suggested that the optimal conditions for attaining the highest level of beta-mannanase were incubation temperature of 32 degrees C, initial moisture level of 59% and aeration rate of 0.5 l/min. A beta-mannanase yield of 2231.26 U/g was obtained when SSF process was carried out under the optimal conditions described above.

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Section: Effect Of Incubation Temperaturesupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Utilization of palm kernel cake for production of β-mannanase by Aspergillus niger FTCC 5003 in solid substrate fermentation using an aerated column bioreactor

Abdeshahian

Samat

Hamid

et al. 2009

J Ind Microbiol Biotechnol

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“…There are many species of fungi reported to produce signifi cantly high mannanase activity. For instance, Lin and Chen ( 2004 ) observed 27.4 U/ml mannanase activity in a submerged culture of Aspergillus niger NCH 189. Similarly, Hossain et al ( 1996 ) obtained about 90 U/ml mannanase activity in submerged conditions using Bacillus sp.…”

Section: Microbial Production Of Mannanasesmentioning

confidence: 99%

Microbial Mannanases: Properties and Applications

Soni

Kango

2013

Advances in Enzyme Biotechnology

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Mannans are a major constituent of the hemicellulose fraction of lignocelluloses. Mannans perform distinct functions as structural components in cell walls of softwoods and storage functions in seeds. Enzymatic hydrolysis of mannan involves the backbone hydrolyzing endo-β-mannanases and β-mannosidases. Mannans are heteropolymeric and their hydrolysis also requires the action of β-glucosidases and side-chain cleaving α-galactosidases and acetyl mannan esterases. Microorganisms are therefore explored for the production of such repertoire of enzymes so that effective mannan hydrolysis can be achieved. The present chapter discusses the occurrence and structural properties of mannans in plant materials and its hydrolysis using enzymes sourced from various fungi and other microorganisms. The production and properties of mannanolytic enzymes, their cloning and expression in heterologous hosts, and their application have also been discussed .

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“…Temperature is one of the main factors affecting the growth of fungi, although these microorganisms have been shown to be able to tolerate a wide range of temperatures, typically from 30 to 40 °C, and some are able to survive in extreme temperatures (≥ 50 °C) (Michael 1972;Smith and Wood 1991;Lin and Chen 2004;Wang et al 2006;Chellapandi and Jani 2009;Sohail et al 2009;Facchini et al 2011). Despite the wide temperature tolerance, the optimum temperature predicted was 30 °C, a temperature that is close to the natural habitat where this fungus was isolated.…”

Section: Optimization Of Multi-enzyme Productionmentioning

confidence: 99%

“…Filamentous fungi have been widely used to produce hydrolytic enzymes for industrial applications. Those belonging to the genus Aspergillus are commonly used for the production of cellulase (Bakir et al 2001), mannanase (Kurakake and Komaki 2001;Lin and Chen 2004;Puchart et al 2004), and xylanase (Lu et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Multi-enzyme Production by Fungi Isolated from Palm Kernel Expeller using Response Surface Methodology

Chen¹,

Liang²,

Jahromi³

et al. 2013

BioResources

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Response surface methodology (RSM) was used to optimize the coproduction of a mixture of crude cellulosic and hemicellulosic enzymes (endoglucanase, xylanase, and mannanase) by Aspergillus terreus K1 in solid-state fermentation (SSF) using palm kernel expeller (PKE) as the sole carbon source. These enzymes have gained renewed interest due to their efficacy to improve the digestibility of PKE for use in diets of mono-gastric animals (poultry, pigs, and fish). The results showed that temperature, moisture, inoculum concentration, and initial pH had significant (P< 0.05) effects on the enzymes production. Using PKE as a solid substrate, maximum endoglucanase, mannanase, and xylanase (17.37, 41.24, and 265.57 U/g DM, respectively) were obtained at 30.5 °C, 62.7% moisture, 6% inoculum, and pH 5.8. The enzyme activities recorded were close to the predicted values (19.97, 44.12, and 262.01 U/g DM, respectively).

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Enhanced mannanase production by submerged culture of Aspergillus niger NCH-189 using defatted copra based media

Cited by 46 publications

References 34 publications

Utilization of palm kernel cake for production of β-mannanase by Aspergillus niger FTCC 5003 in solid substrate fermentation using an aerated column bioreactor

Utilization of palm kernel cake for production of β-mannanase by Aspergillus niger FTCC 5003 in solid substrate fermentation using an aerated column bioreactor

Microbial Mannanases: Properties and Applications

Optimization of Multi-enzyme Production by Fungi Isolated from Palm Kernel Expeller using Response Surface Methodology

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