Enhanced Luminol-Dependent Chemiluminescence of Stimulated Rat Alveolar Macrophages by Pretreatment With Alveolar Lining Material

Webb, Deborah; Jeska, Edward L.

doi:10.1002/jlb.40.1.55

Cited by 29 publications

(13 citation statements)

References 14 publications

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“…It has been shown that the amount of CL produced depends on the extent of ingested particles (29). We did not observe an increased generation of CL in monocytes preincubated with Curosurf, although this has previously been described in rat alveolar macrophages pretreated with several unsaturated lipids (30). In a recent study, a suppressive effect of rabbit lung surfactant on CL generated by rabbit alveolar macrophages was demonstrated; again, lipid components were thought to be responsible for this alteration of cell function (3 1).…”

Section: Discussioncontrasting

confidence: 78%

Phagocytic Functions and Tumor Necrosis Factor Secretion of Human Monocytes Exposed to Natural Porcine Surfactant (Curosurf)

et al. 1991

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ABSTRACT. In this study we have analyzed various phagocytic functions and tumor necrosis factor (TNF) secretion of human monocytes exposed to either a biochemically well-defined porcine surfactant or a purified phospholipid preparation. Adherence, random migration, and chemotactic response to zymosan activated serum and formyl-methionyl-leucyl-phenylalanine were normal in surfactanttreated monocytes; surfactant was not a chemotactic stimulus. In contrast, phagocytosis of Staphy?vlococcus aureus by monocytes exposed to surfactant (100 pg/mL) or phospholipids (100 pg/mL) was slightly impaired [surfactant:at 30 min (t30) 48.5 f 11%, t o 73.3 + 10.1%; phospholipids: t30 47.3 f 2.5%, Go 68.0 f 6.6%; controls: t30 66.6 + 9.9%, 81.0 f 6.6%, p < 0.05 at t30 for both,p < 0.05 at for phospholipids]. Due to the smaller number of S. aureus ingested, bactericidal activity of surfactant-or phospholipid-treated monocytes was slightly reduced when compared with controls. Surfactant or phospholipids had no bactericidal activity. Uptake of Candida albicans was identical in surfactant-or phospholipid-treated monocytes and untreated controls; the same was true for the number of Candida organisms ingested per cell. Phagocytosisassociated chemiluminescence and production of superoxide anion by monocytes of either source in response to phorbol myristate acetate and opsonized zymosan were also unaffected. Surfactant or phospholipids (500 pg/mL), however, effectively suppressed TNF secretion by resting and by lipopolysaccharide (LPS)-stimulated monocytes in a dose-dependent fashion, (LPS-stimulated monocyte controls: 3004 f 570 pg/mL; LPS + surfactant: 426 + 162 pg/mL; LPS + phospholipids: 28 f 9.6 pg/mL; p < 0.001 for both). TNF is an important mediator of inflammation, and our data suggest that surfactant or phospholipids, by suppressing monocyte TNF secretion, may have an important role in down-regulating inflammatory reactions in the lung. Supported by a grant from Deutsche Forschungsgemeinschaft (Sp 23912-3) (C.P.S.).Surfactant replacement therapy in severe neonatal respiratory distress syndrome effectively reduces respiratory distress syndrome-associated pulmonary morbidity and mortality in preterm infants. Immediately after intrabronchial administration of natural surfactant (human, bovine, porcine), there is a dramatic increase in oxygenation with a subsequent reduction in ventilatory requirements (1). However, there is only limited information about the effect of natural surfactant on human pulmonary monocytes and alveolar macrophages. These cells have an essential role in host defense (2, 3) as well as in the regulation of immune functions and inflammation (4-9). Available data on macrophage-surfactant interaction have been mostly derived from in vitro experiments using lung lavage materials from various species. However, most investigators have focused on a single aspect of macrophage function.Our present study was initiated to examine the principal cell functions of monocytes-the macrophage precursors-in response to inflamma...

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Section: Discussioncontrasting

confidence: 78%

Phagocytic Functions and Tumor Necrosis Factor Secretion of Human Monocytes Exposed to Natural Porcine Surfactant (Curosurf)

et al. 1991

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“…Moreover, the active concentrations of the surfactant preparations (100 -500 g/ml) are consistent with reported in vivo concentrations (20). Surfactant has previously been reported to enhance (42), suppress (20,21), or have no effect on the production of superoxide by alveolar macrophages or monocytes (34). However, the different models and methods used have probably contributed to the conflicting reports.…”

Section: Discussionsupporting

confidence: 60%

Surfactant phospholipid DPPC downregulates monocyte respiratory burst via modulation of PKC

Tonks

Parton²,

Tonks³

et al. 2005

American Journal of Physiology-Lung Cellular and Molecular Phys

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Pulmonary surfactant phospholipids have been shown previously to regulate inflammatory functions of human monocytes. This study was undertaken to delineate the mechanisms by which pulmonary surfactant modulates the respiratory burst in a human monocytic cell line, MonoMac-6 (MM6). Preincubation of MM6 cells with the surfactant preparations Survanta, Curosurf, or Exosurf Neonatal inhibited the oxidative response to either lipopolysaccharide (LPS) and zymosan or phorbol 12-myristate 13-acetate (PMA) by up to 50% ( P < 0.01). Preincubation of MM6 cells and human peripheral blood monocytes with dipalmitoyl phosphatidylcholine (DPPC), the major phospholipid component of surfactant, inhibited the oxidative response to zymosan. DPPC did not directly affect the activity of the NADPH oxidase in a MM6 reconstituted cell system, suggesting that DPPC does not affect the assembly of the individual components of this enzyme into a functional unit. The effects of DPPC were evaluated on both LPS/zymosan and PMA activation of protein kinase C (PKC), a ubiquitous intracellular kinase, in MM6 cells. We found that DPPC significantly inhibited the activity of PKC in stimulated cells by 70% ( P < 0.01). Western blotting experiments demonstrated that DPPC was able to attenuate the activation of the PKCδ isoform but not PKCα. These results suggest that DPPC, the major component of pulmonary surfactant, plays a role in modulating leukocyte inflammatory responses in the lung via downregulation of PKC, a mechanism that may involve the PKCδ isoform.

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“…Since natural surfactant was more active, surfactant apoprote'ins were also believed to be involved in this process. In contrast, Webb and Jeska [21] found enhanced luminol-dependent CL of rat alveolar macrophages by pretreatment with the lipid fraction from the alveolar lining material and subsequent stimulation with latex beads. Van Iwaarden et al [22] recently published data, indicating an increase of lucigenin-dependent CL of rat alveolar macrophages by human SP-A and rat surfactant without further stimuli.…”

Section: Discussionmentioning

confidence: 81%

Effect of canine surfactant protein (SP‐A) on the respiratory burst of phagocytic cells

et al. 1990

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Cells obtained from bronchoalveolar lavage, or neutrophils of peripheral blood of dog, were incubated with the canine surfactant-associated protein A @P-A). A significant decrease of the production of superoxide anion was observed after subsequent stimulation with phorbol-12-myristate-13-acetate (PMA) as measured by the lucigenin-dependent chemiluminescence (CL). Several other proteins used for control experiments did not decrease lucigenin-dependent CL, indicating a specific effect of SP-A on phagocytes. Treatment of SP-A with collagenase. prior to incubation with neutrophils destroyed the depleting effect on oxygen radical production of PMA-stimulated cells. We propose that SP-A acts as a regulatory factor of the respitatory burst of alveolar macrophages and neutrophils in the lungs. The inhibitory effect of SP-A is down-regulated by collagenase released from stimulated alveolar macrophages.

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Enhanced Luminol-Dependent Chemiluminescence of Stimulated Rat Alveolar Macrophages by Pretreatment With Alveolar Lining Material

Cited by 29 publications

References 14 publications

Phagocytic Functions and Tumor Necrosis Factor Secretion of Human Monocytes Exposed to Natural Porcine Surfactant (Curosurf)

Phagocytic Functions and Tumor Necrosis Factor Secretion of Human Monocytes Exposed to Natural Porcine Surfactant (Curosurf)

Surfactant phospholipid DPPC downregulates monocyte respiratory burst via modulation of PKC

Effect of canine surfactant protein (SP‐A) on the respiratory burst of phagocytic cells

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