Enhanced isolation of Serpulina hyodysenteriae by using sliced agar media

Olson, L. D.

doi:10.1128/jcm.34.12.2937-2941.1996

Cited by 13 publications

(6 citation statements)

References 4 publications

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“…The presence of low, flat, spreading growth (typical of spirochetes) on the plate was recorded, as was the extent of hemolysis. Areas of suspected spirochetal growth were transferred to sliced blood agar (BBL, Becton-Dickinson) as described previously 18 and incubated under the same conditions for 3 d. Suspected spirochetal growth displaying strong hemolysis migrating along the lines of the sliced agar farthest from the initial streaking point were transferred to anaerobic basal agar (Oxoid) supplemented with 5% (v/v) defibrinated sheep blood and colistin (10 mg/L) and incubated anaerobically for 3 d. Bacteria from the final selective agar were then identified by MALDI-TOF MS.…”

Section: Bacteriologic Examinationsmentioning

confidence: 99%

Brachyspira hyodysenteriae detection in the large intestine of slaughtered pigs

et al. 2017

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Detection of subclinical Brachyspira hyodysenteriae infection in pig herds using feces is challenging. However, the ability to detect the pathogen in intestinal samples of slaughtered pigs has not been investigated, to our knowledge. Therefore, we determined the detection of B. hyodysenteriae in the colon, cecum, and rectum from slaughtered pigs. We analyzed the correlation between detection rates and intestinal lesions, ingesta or fecal consistency, and time from sample collection until processing. A total of 400 ingesta-mucosal (colon, cecum) and 200 fecal (rectum) samples from 200 pigs originating from 20 different herds were bacteriologically examined using selective culture followed by Brachyspira spp. identification by PCR and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Ingesta or fecal consistency and intestinal lesions were scored. Brachyspira hyodysenteriae was detected in 23 samples from 16 intestines originating from 7 herds. Brachyspira spp. were detected in 96 samples. More intestinal (16) than fecal (7) samples tested positive for B. hyodysenteriae. For Brachyspira spp., this difference was significant (69 vs. 27; p < 0.01). In particular, colon samples tested positive ( n = 42, p = 0.06). Most (91%) of the intestines showed no lesions typical for clinical B. hyodysenteriae infection, and median ingesta or fecal consistency was "soft and formed," indicating subclinical infection, colonization, or absence of infection. Ingesta from slaughtered pigs, in particular from the colon and sites with lesions, is useful material for detection of B. hyodysenteriae.

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Section: Bacteriologic Examinationsmentioning

confidence: 99%

Brachyspira hyodysenteriae detection in the large intestine of slaughtered pigs

et al. 2017

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“…The selective culture and the biochemical differentiation for Brachyspira spp. were performed as previously described [ 14 , 8 , 26 ]. The primary cultures from intestinal scrapings were studied also by B. pilosicoli -specific polymerase chain reaction (PCR) assays targeting 16S rDNA and 23S rDNA [ 9 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

Neither Hippurate-negative Brachyspira pilosicoli nor Brachyspira pilosicoli Type Strain Caused Diarrhoea in Early-weaned Pigs by Experimental Infection

Fossi

Ahlsten

Pohjanvirta³

et al. 2005

Acta Vet Scand

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Sukura A, Pelkola K, Pelkonen S: Neither hippurate-negative Brachyspira pilosicoli nor Brachyspira pilosicoli type strain caused diarrhoea in early-weaned pigs by experimental infection. Acta vet. scand. 2005, 46, 257-267. -A hippurate-negative biovariant of Brachyspira pilosicoli (B. pilosicoli hipp-) is occasionally isolated in diarrhoeic pigs in Finland, often concomitantly with hippurate-positive B. pilosicoli or Lawsonia intracellularis. We studied pathogenicity of B. pilosicoli hipp-with special attention paid to avoiding co-infection with other enteric pathogens. Pigs were weaned and moved to barrier facilities at the age of 11 days. At 46 days, 8 pigs were inoculated with B. pilosicoli hipp-strain Br1622, 8 pigs were inoculated with B. pilosicoli type strain P43/6/78 and 7 pigs were sham-inoculated. No signs of spirochaetal diarrhoea were detected; only one pig, inoculated with P43/6/78, had soft faeces from day 9 to 10 post inoculation. The pigs were necropsied between days 7 and 23 after inoculation. Live pigs were culturenegative for Brachyspira spp., but B. pilosicoli hipp-was reisolated from necropsy samples of two pigs. The lesions on large colons were minor and did not significantly differ between the three trial groups. In silver-stained sections, invasive spirochaetes were detected in colonic mucosae of several pigs in all groups. Fluorescent in situ hybridisation for genus Brachyspira, B. pilosicoli and strain Br1622 was negative. However, in situ detection for members of the genus Leptospira was positive for spirochaete-like bacteria in the colonic epithelium of several pigs in both infected groups as well as in the control group. L. intracellularis, Salmonella spp., Yersinia spp. and intestinal parasites were not detected. The failure of B. pilosicoli strains to cause diarrhoea is discussed with respect to infectivity of the challenge strains, absence of certain intestinal pathogens and feed and management factors.Brachyspira pilosicoli, intestinal spirochaetosis, spirochaetal colitis, pig, diarrhoea, experimental infection, early weaning, Lawsonia intracellularis.

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“…The samples were cultivated by routine methods (Jenkinson and Wingar, 1981;Olson, 1996). After 14 days of incubation, the primary plates from the last three samplings were also studied with B. pilosicoli-specific PCR assays targeting 16S rDNA and 23S rDNA (Fellström et al, 1997;Leser et al, 1997).…”

Section: Methodsmentioning

confidence: 99%

Eradication of endemicBrachyspira pilosicoliinfection from a farrowing herd: a case report

Fossi¹,

Heinonen

Pohjanvirta³

et al. 2001

Anim. Health. Res. Rev.

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Brachyspira pilosicoli and B. innocens were isolated repeatedly from a herd of 60 sows which mostly produced feeder pigs but also raised some fattening pigs. Postweaning diarrhea had been a severe problem in this herd for years. The B. pilosicoli eradication plan was based on the general guidelines for elimination of B. hyodysenteriae, with some modifica-tions. The eradication measures were run in August 1997. In-feed medication with 200p.p.m. tiamulin lasted for 18–30 days, depending on the age group. The piggery unit was emptied, cleaned, disinfected and dried, and all worn surfaces were repaired. The animals were removed to temporary sheds situated 0–100m from the piggery unit. Only the sows and the boar returned to the piggery unit. All other pigs were sold from the sheds within 3 months after the eradication. Immediately after the eradication, the clinical postweaning diarrhea disappeared. The success of the program was monitored four times bacteriologically, and the last control sampling was in December 1999, 7 months after the total withdrawal of antimicrobial feed additives. The primary cultures from the last three samplings were also analysed with B. pilosicoli-specific PCR. All the samples were negative for B. pilosicoli. However, B. innocens could be isolated from each batch of samples. The analysis of B. inno-cens isolates by pulsed-field gel electrophoresis indicated that at least one genotype persisted in the herd. The clinical and laboratory findings suggest that the eradication of B. pilosicoli had succeeded in this herd

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Enhanced isolation of Serpulina hyodysenteriae by using sliced agar media

Cited by 13 publications

References 4 publications

Brachyspira hyodysenteriae detection in the large intestine of slaughtered pigs

Brachyspira hyodysenteriae detection in the large intestine of slaughtered pigs

Neither Hippurate-negative Brachyspira pilosicoli nor Brachyspira pilosicoli Type Strain Caused Diarrhoea in Early-weaned Pigs by Experimental Infection

Eradication of endemicBrachyspira pilosicoliinfection from a farrowing herd: a case report

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