Enhanced inulinase production by Fusarium solani JALPK from invasive weed using response surface methodology

Kamble, Prajakta P.; Suryawanshi, Sachin R.; Jadhav, Jyoti P.; Attar, Yasmin C.

doi:10.1016/j.mimet.2019.02.021

Cited by 22 publications

(12 citation statements)

References 32 publications

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“…Similar shaped osazones were visualized under (40x) magni cation in a light microscope for both the standard fructose and the test broth. Similar shaped osazones were also reported by Hassid and McCready (1942) [30], Kamble et al, (2019) [12], Das et al, (2019) [13].…”

Section: Osazone Formation Testsupporting

confidence: 87%

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Response surface methodology to optimize inulinase production by a newly isolated Penicillium amphipolaria strain by solid-state fermentation of Saccharum arundinaceum

Das¹,

Bhat²,

Selvaraj³

2020

Preprint

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In the present investigation, a new fungal inulinase producer Penicillium amphipolaria KAS 2555 has been isolated from the soil of dead mangroves litter area, followed by the inulinase production and optimization by solid-state fermentation using a low-cost substrate – hardy sugarcane (Saccharum arundinaceum). While screening, only Penicillium amphipolaria KAS 2555 showed the hydrolysis zone on the plate containing inulin media. The exoinulolytic nature of inulinase and its form of action was confirmed by thin-layer chromatography (TLC). After 96 h of the fermentation period, an activity of 2.45 U/gds was obtained. The I/S ratio of 0.59 proved that the enzyme is inulolytic in nature. Media optimization was performed to obtain a regression model using Central Composite Design (CCD). For optimization, five significant media components viz., inulin, (NH4)2SO4, K2HPO4, KH2PO4 and, NaCl were used. A 3.10-fold increase in activity of inulinase (7.59 U/gds) was obtained under the optimal settings of (g/gds) inulin- 0.1, (NH4)2SO4- 0.002, K2HPO4- 0.1, KH2PO4- 0.02 and NaCl- 0.02.

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Section: Osazone Formation Testsupporting

confidence: 87%

“…Inulinase producing microbes have been widely isolated from sources like marine soil [10], rotten wood [11], inulin rich plants [12], etc. Mangrove soil is also proved to be an e cient source of inulinase producing fungi [13].…”

Section: Introductionmentioning

confidence: 99%

Response surface methodology to optimize inulinase production by a newly isolated Penicillium amphipolaria strain by solid-state fermentation of Saccharum arundinaceum

Das¹,

Bhat²,

Selvaraj³

2020

Preprint

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“…Well diffusion method is widely used to evaluate the antifungal and antimicrobial activity of different nanoparticles prepared of silver and palladium. Similarly to the procedure used in agar welldiffusion technique, the PDA plates were prepared using submerged inoculation using two fungal strains: Aspergillus niger (NCIM 1360) and Fusarium solani JALPK [11]. The palladium nanoparticles (100 μL), of different concentrations were introduced in the well, these agar plates were then incubated under desire temperature.…”

Section: Antifungal Activitymentioning

confidence: 99%

“…Thus, magical properties are developed in materials in nano regime. Nanoparticles are of great scientific interest as they bridge the gap between bulk materials and atomic or molecular structures [9][10][11]. A bulk material has constant physical properties regardless of its size, but at nano-scale this is often not the case.…”

Section: Introductionmentioning

confidence: 99%

Bio-inspired synthesis of catalytically and biologically active palladium nanoparticles using Bos taurus urine

et al. 2020

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Physiologically processed bio-molecules present in cow excreta can be used to reduce metal ions into metal nanoparticles in a single step by novel synthetic routes. This biogenic reduction of metal ion to base metal is quite rapid and easily scaled up. These NPs exhibit excellent catalytic activities for studied organic reactions. X-ray diffraction pattern of reaction product confirmed the formation of palladium nanoparticles. The synthesized PdNPs shows potential activity for organic transformation. Antimicrobial activity of nanoparticles determines biogenic potential of the nanoparticles against different microorganisms and antioxidant activity determined the potential of free radicals. Both of the antimicrobial and antioxidant activity helps in study application of nanoparticles in medical and veterinary sciences.

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“…The C.V.% of 3.69 indicates the experiments were highly reliable. Diagnostic plots were drawn to assess how well the model approximated the true response surface [45], and some selected plots are shown in Figure 4. The normal probability of residuals (Figure 4(a)) was Table 7.…”

Section: Optimization By Rsmmentioning

confidence: 99%

Isolation and identification of a psychrotolerant dimethyl phthalate-degrading bacterium from selected frozen soil of high-latitude areas in China and optimization of its fermentation conditions using response surface methodology

Ji-xian

Wang

et al. 2019

Biotechnology & Biotechnological Equipment

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Enhanced inulinase production by Fusarium solani JALPK from invasive weed using response surface methodology

Cited by 22 publications

References 32 publications

Response surface methodology to optimize inulinase production by a newly isolated Penicillium amphipolaria strain by solid-state fermentation of Saccharum arundinaceum

Response surface methodology to optimize inulinase production by a newly isolated Penicillium amphipolaria strain by solid-state fermentation of Saccharum arundinaceum

Bio-inspired synthesis of catalytically and biologically active palladium nanoparticles using Bos taurus urine

Isolation and identification of a psychrotolerant dimethyl phthalate-degrading bacterium from selected frozen soil of high-latitude areas in China and optimization of its fermentation conditions using response surface methodology

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