Enhanced initial bone regeneration with inorganic polyphosphate-adsorbed hydroxyapatite

Morita, Koji; Doi, Katsuhiko; Kubo, Takayasu; Takeshita, Ryou; Kato, Shinichi; Shiba, Toshikazu; Akagawa, Yasumasa

doi:10.1016/j.actbio.2009.12.055

Cited by 38 publications

(35 citation statements)

References 23 publications

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“…We were initially concerned that the interconnected porous structure of IP-CHA would be disrupted by adsorbed poly(P), but SEM evaluation showed no changes in the characteristic three-dimensional structure of the interconnected pores known to be critical for osteoconduction. These results coincided with that of the adsorbed poly(P) onto IP-CHA 13) . Mechanical strength also did not influence the adsorption process.…”

Section: Discussionsupporting

confidence: 73%

“…poly(P) appears to enhance bone regeneration by facilitating this process. The amount of poly(P) adsorbed onto IP-CHA is 41.2 mg poly(P)/mg IP-CHA 13) . The mechanism of adsorption of poly(P) onto IP-CHA surface is a weak electrical bonding between the negatively-charged poly(P) and the positively-charged hydroxyapatite.…”

Section: Discussionmentioning

confidence: 99%

“…High levels of new bone formation were observed in Poly(P)/IP-CHA. onto block type IP-CHA composites accelerated early bone regeneration in a rabbit femur model 12,13) . Blocks of bone graft material are difficult to apply for implant GBR because of the complex topography of the bone defect at the implant site.…”

Section: Discussionmentioning

confidence: 99%

“…It is considered that Poly(P)/IP-CHA has ability both osteoconduction and osteroinduction in bone regeneration. Our previous study showed that adsorption per unit weight of IP-CHA was not significantly influenced by the block size in the range of 35.3 mm 3 (3.0 mm in length, 5.0 mm in diameter) to 785 mm 3 (10 mm in length, 10 mm in diameter) 13) . Thus, we speculated that poly(P) would be uniformly adsorbed onto IP-CHA granules as small as 500 μm.…”

Section: Discussionmentioning

confidence: 99%

“…On the basis of these preclinical studies suggesting that poly(P) promotes cell proliferation and osteogenic differentiation, we have been developing bone graft biomaterials containing poly(P) for accelerated bone regeneration. In our previous studies, we demonstrated that blocks of interconnected porous calcium hydroxyapatite (IP-CHA) 11) with adsorbed poly(P) accelerated bone formation 12,13) . However, blocks of graft material are difficult to apply in clinical GBR because the topology of the dental bone defect caused by surgical resection and subsequent inflammation is complex and not predictable before treatment.…”

Section: Introductionmentioning

confidence: 99%

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Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

et al. 2014

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Inorganic polyphosphate (poly(P)) is recognized as a therapeutic agent that promotes fibroblast growth factor and enhances osteogenic differentiation, and in vivo, when adsorbed onto interconnected porous calcium hydroxyapatite (IP-CHA) enhances bone regeneration. The present study focused on the effect of poly(P) adsorbed onto IP-CHA granules (Poly(P)/IP-CHA) in guided bone regeneration (GBR). Dental implants were placed into the edentulous mandibular areas of five Beagle-Labrador hybrid dogs with screw expose on the buccal side, and then bone defects were filled Poly(P)/IP-CHA (test) or IP-CHA (control). After 12 weeks, histological evaluation and histomorphometrical analysis were performed. Newly-bone formation around exposed implant screw was clearly detected in the test-group. The ratio for regenerated bone height in the test group versus the control-group was 85.6±20.2 and 62.6±23.8, respectively, with no significant difference, while, that for bone implant contact was significantly higher (67.9±11.8 and 48.8±14.1, respectively). These findings indicate that Poly(P)/IP-CHA enhances bone regeneration in GBR.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

et al. 2014

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Cellular calcification induced by inorganic polyphosphate involves ATP depletion and opening of the mitochondrial permeability transition pore (mPTP)

Tsutsumi

Sasase

2019

FEBS Open Bio

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Inorganic polyphosphate (polyP) is a linear polymer containing tens to hundreds of orthophosphate residues linked by high‐energy phosphoanhydride bonds. PolyP promotes osteocalcification and bone mineralization in both mouse and human osteoblastic cells. In the present study, we examined the molecular mechanism by which polyP affects mitochondrial metabolism to promote cellular calcification in MC 3T3‐E1 osteoblastic cells. The cellular content of adenosine triphosphate ( ATP ) was diminished one day after polyP treatment, and this was accompanied by increased conversion to adenosine diphosphate. Furthermore, mitochondrial membrane potential was significantly decreased in polyP‐treated cells. These results suggest that the depletion of intracellular ATP and the decrease in mitochondrial membrane potential induced by polyP treatment may be a trigger to promote cell calcification.

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Hydroxyapatite with Permanent Electrical Polarization: Preparation, Characterization, and Response against Inorganic Adsorbates

et al. 2018

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Permanently polarized hydroxyapatite (HAp) particles have been prepared by applying a constant DC of 500 V at 1000 °C for 1 h to the sintered mineral. This process causes important chemical changes, as the formation of OH defects (vacancies), the disappearance of hydrogenophosphate ions at the mineral surface layer, and structural variations reflected by the increment of the crystallinity. As a consequence, the electrochemical properties and electrical conductivity of the polarized mineral increase noticeably compared with as-prepared and sintered samples. Moreover, these increments remain practically unaltered after several months. In addition, permanent polarization favours significantly the ability of HAp to adsorb inorganic bioadsorbates in comparison with as-prepared and sintered samples. The adsorbates cause a significant increment of the electrochemical stability and electrical conductivity with respect to bare polarized HAp, which may have many implications for biomedical applications of permanently polarized HAp.

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Enhanced initial bone regeneration with inorganic polyphosphate-adsorbed hydroxyapatite

Cited by 38 publications

References 23 publications

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

Cellular calcification induced by inorganic polyphosphate involves ATP depletion and opening of the mitochondrial permeability transition pore (mPTP)

Hydroxyapatite with Permanent Electrical Polarization: Preparation, Characterization, and Response against Inorganic Adsorbates

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