Enhanced
 in vitro
 antimicrobial activity of amphotericin B with berberine against dual‐species biofilms of
 Candida albicans
 and
 Staphylococcus aureus

Gao, Shanjun; Zhang, Shuzhen

doi:10.1111/jam.14872

Cited by 22 publications

(7 citation statements)

References 42 publications

(48 reference statements)

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“…aureus [21]. Some other studies report the inhibitory effect of berberine on developed biofilm, in which biofilm exposed to berberine near MIC for 12–24 h shows a significant decrease in biomass [22, 23].…”

Section: Resultsmentioning

confidence: 99%

“…A previous investigation reports that at sub-inhibitory concentration, berberine increases the final biofilm biomass of some strains while decreasing it of other strains, suggesting the strain-dependent effect of berberine on biofilm formed by S. aureus [21]. Some other studies report the inhibitory effect of berberine on developed biofilm, in which biofilm exposed to berberine near MIC for 12-24 h shows a significant decrease in biomass [22,23].…”

Section: Berberine Inhibits Biofilm Dispersal At Sub-inhibitory Conce...mentioning

confidence: 90%

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Berberine at sub-inhibitory concentration inhibits biofilm dispersal in Staphylococcus aureus

et al. 2022

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Staphylococcus aureus is a major human pathogen, which has multiple drug resistance and can cause serious infections. Recent studies have shown that berberine has antibacterial activity and it can affect biofilm formation of S. aureus . However, the berberine effect on the biofilm of S. aureus is controversial. In this study, we investigate the effect of berberine on the biofilm development in S. aureus NCTC8325 and explore the possible mechanism. Susceptibility test shows that berberine inhibits growth of methicillin-sensitive S. aureus (MSSA), methicillin-resistant S. aureus (MRSA) and vancomycin-intermediate S. aureus (VISA) at different concentrations. S. aureus NCTC8325 is chosen as a model strain to explore further the berberine effect. The MIC of berberine for S. aureus NCTC8325 is 256 µg ml−1. Berberine below 32 µg ml−1 inhibits the dispersal of biofilm and stimulates clumping of cells of NCTC8325 in a concentration-dependent manner, while not showing obvious inhibition on the bacterial growth. The transcription of the key negative regulator of biofilm dispersal AgrA is decreased and an agrA mutant forms biofilm reaching to a similar level of biomass to WT in the presence of berberine at 32 µg ml−1. Transcription of some genes involving synthesis of biofilm structure components, including polysaccharide intracellular adhesin (PIA), proteins and eDNA were also up-regulated, especially icaA for PIA synthesis. And consistently, PIA content was increased in cells exposed to berberine at 32 µg ml−1. This study reveals the dependence of berberine inhibition of biofilm dispersal on the Agr system, which is the first report exploring the molecule mechanism of the berberine effect on the biofilm of S. aureus .

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Section: Resultsmentioning

confidence: 99%

Section: Berberine Inhibits Biofilm Dispersal At Sub-inhibitory Conce...mentioning

confidence: 90%

Berberine at sub-inhibitory concentration inhibits biofilm dispersal in Staphylococcus aureus

et al. 2022

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“…Eugenol with fluconazole or azithromycin also exhibited enhanced antibiofilm activity against C. albicans-S. mutans biofilm (Jafri et al, 2020). In addition, 2-aminobenzimidazole with curcumin (Tan et al, 2019), berberine and amphotericin B (Gao et al, 2021), anidulafungin and tigecycline (Rogiers et al, 2018), and fluconazole and minocycline (Li et al, 2015) were effective in deterring C. albicans-S. aureus biofilm. Interestingly, some antifungal agents are repurposed to treat C. albicans involved cross-kingdom biofilms.…”

Section: Synthetic Antibiofilm Productsmentioning

confidence: 98%

“…mutans biofilm (Jafri et al ., 2020). In addition, 2‐aminobenzimidazole with curcumin (Tan et al ., 2019), berberine and amphotericin B (Gao et al ., 2021), anidulafungin and tigecycline (Rogiers et al ., 2018), and fluconazole and minocycline (Li et al ., 2015) were effective in deterring C . albicans–S .…”

Section: Therapeutic Approaches For Candida–bacterial Biofilm‐associa...mentioning

confidence: 99%

In it together: Candida–bacterial oral biofilms and therapeutic strategies

Hwang

2022

Environ Microbiol Rep

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Summary Under natural environmental settings or in the human body, the majority of microorganisms exist in complex polymicrobial biofilms adhered to abiotic and biotic surfaces. These microorganisms exhibit symbiotic, mutualistic, synergistic, or antagonistic relationships with other species during biofilm colonization and development. These polymicrobial interactions are heterogeneous, complex and hard to control, thereby often yielding worse outcomes than monospecies infections. Concerning fungi, Candida spp., in particular, Candida albicans is often detected with various bacterial species in oral biofilms. These Candida‐bacterial interactions may induce the transition of C. albicans from commensal to pathobiont or dysbiotic organism. Consequently, Candida–bacterial interactions are largely associated with various oral diseases, including dental caries, denture stomatitis, periodontitis, peri‐implantitis, and oral cancer. Given the severity of oral diseases caused by cross‐kingdom consortia that develop hard‐to‐remove and highly drug‐resistant biofilms, fundamental research is warranted to strategically develop cost‐effective and safe therapies to prevent and treat cross‐kingdom interactions and subsequent biofilm development. While studies have shed some light, targeting fungal‐involved polymicrobial biofilms has been limited. This mini‐review outlines the key features of Candida–bacterial interactions and their impact on various oral diseases. In addition, current knowledge on therapeutic strategies to target Candida–bacterial polymicrobial biofilms is discussed.

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“…Berberine has been reported to have antibacterial efficacy in eliminating bacterial and fungal biofilms. As such, berberine exerted synergistic effects on inhibiting Candida albicans / S. aureus dual strain biofilms in combination with amphotericin B, an efficient antibiotic that utilized for the treatment of fungal infections in clinic ( Gao et al, 2021 ). A study by Ning et al demonstrated that berberine inhibited biofilm formation via downregulation of the expression of the QS regulatory gene agrA in a concentration-dependent manner in S. aureus ( Ning et al, 2022 ).…”

Section: Quorum Sensingmentioning

confidence: 99%

Recent advances on the regulation of bacterial biofilm formation by herbal medicines

Zhang

Wang

et al. 2022

Front. Microbiol.

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Biofilm formation is a fundamental part of life cycles of bacteria which affects various aspects of bacterial-host interactions including the development of drug resistance and chronic infections. In clinical settings, biofilm-related infections are becoming increasingly difficult to treat due to tolerance to antibiotics. Bacterial biofilm formation is regulated by different external and internal factors, among which quorum sensing (QS) signals and nucleotide-based second messengers play important roles. In recent years, different kinds of anti-biofilm agents have been discovered, among which are the Chinese herbal medicines (CHMs). CHMs or traditional Chinese medicines have long been utilized to combat various diseases around the world and many of them have the ability to inhibit, impair or decrease bacterial biofilm formation either through regulation of bacterial QS system or nucleotide-based second messengers. In this review, we describe the research progresses of different chemical classes of CHMs on the regulation of bacterial biofilm formation. Though the molecular mechanisms on the regulation of bacterial biofilm formation by CHMs have not been fully understood and there are still a lot of work that need to be performed, these studies contribute to the development of effective biofilm inhibitors and will provide a novel treatment strategy to control biofilm-related infections.

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Enhanced in vitro antimicrobial activity of amphotericin B with berberine against dual‐species biofilms of Candida albicans and Staphylococcus aureus

Cited by 22 publications

References 42 publications

Berberine at sub-inhibitory concentration inhibits biofilm dispersal in Staphylococcus aureus

Berberine at sub-inhibitory concentration inhibits biofilm dispersal in Staphylococcus aureus

In it together: Candida–bacterial oral biofilms and therapeutic strategies

Recent advances on the regulation of bacterial biofilm formation by herbal medicines

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