Enhanced hydrolysis of lignocellulosic biomass: Bi‐functional enzyme complexes expressed in <i>Pichia pastoris</i> improve bioethanol production from <i>Miscanthus sinensis</i>

Shin, Seung-Soo; Hyeon, Jeong Eun; Kim, Young In; Kang, Dea Hee; Kim, Seung Wook; Park, Chulhwan; Han, Sung Ok

doi:10.1002/biot.201500081

Cited by 18 publications

(8 citation statements)

References 28 publications

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“…Additionally, chimeric enzymes with dockerin domains have also been used to assemble complexes via cohesin‐dockerin interactions . Previous reports have described the use of this interaction between dockerin and cohesin for the construction of protein complexes composed of endoglucanase or xylanase with increased hydrolytic ability with cellulose or hemicellulose as substrates, respectively .…”

Section: Protein Scaffolds For Biomass Conversion and Immobilizationmentioning

confidence: 99%

“…Previous reports have demonstrated that enzyme complexes enabled the effective targeting and concentration of the hydrolytic action through enzyme proximity effect by a coordinated organization . Additionally, these minicellulosomes could be recycled by single‐step CBM‐based recycling methods .…”

Section: Protein Scaffolds For Biomass Conversion and Immobilizationmentioning

confidence: 99%

“…Additionally, chimeric endoglucanase cCelE and xylanase XynB were constructed as a complexed system with miniCbpA as scaffoldin. These enzyme complexes showed the effective degradation of lignocellulosic biomass with the aid of the CBM for efficient substrate affinity . As the construction of multi‐enzyme complexes has been shown to affect cellulose hydrolysis, the combination of cellulases with scaffolding subunits may be an effective means of achieving efficient degradation .…”

Section: Protein Scaffolds For Biomass Conversion and Immobilizationmentioning

confidence: 99%

“…Many approaches containing immobilization systems on cell surface or extracellular secretion systems, have been implemented for the utilization of biomass through biological enzyme processing . The use of a scaffold is one strategy that can be used for effective lignocellulosic biomass hydrolysis . The term ”scaffold“ implies the formation of stable complexes, which are further reinforced by highly specific localization .…”

Section: Introductionmentioning

confidence: 99%

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Design of nanoscale enzyme complexes based on various scaffolding materials for biomass conversion and immobilization

Hyeon

Shin

Han

2016

Biotechnology Journal

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The utilization of scaffolds for enzyme immobilization involves advanced bionanotechnology applications in biorefinery fields, which can be achieved by optimizing the function of various enzymes. This review presents various current scaffolding techniques based on proteins, microbes and nanomaterials for enzyme immobilization, as well as the impact of these techniques on the biorefinery of lignocellulosic materials. Among them, architectural scaffolds have applied to useful strategies for protein engineering to improve the performance of immobilized enzymes in several industrial and research fields. In complexed enzyme systems that have critical roles in carbon metabolism, scaffolding proteins assemble different proteins in relatively durable configurations and facilitate collaborative protein interactions and functions. Additionally, a microbial strain, combined with designer enzyme complexes, can be applied to the immobilizing scaffold because the in vivo immobilizing technique has several benefits in enzymatic reaction systems related to both synthetic biology and metabolic engineering. Furthermore, with the advent of nanotechnology, nanomaterials possessing ideal physicochemical characteristics, such as mass transfer resistance, specific surface area and efficient enzyme loading, can be applied as novel and interesting scaffolds for enzyme immobilization. Intelligent application of various scaffolds to couple with nanoscale engineering tools and metabolic engineering technology may offer particular benefits in research.

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Section: Protein Scaffolds For Biomass Conversion and Immobilizationmentioning

confidence: 99%

Section: Protein Scaffolds For Biomass Conversion and Immobilizationmentioning

confidence: 99%

Section: Protein Scaffolds For Biomass Conversion and Immobilizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Design of nanoscale enzyme complexes based on various scaffolding materials for biomass conversion and immobilization

Hyeon

Shin

Han

2016

Biotechnology Journal

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“…ZHA et al (2013) identificaram uma cepa de Pichia anomala como capaz de assimilar pentoses e promissora para produção de bioetanol a partir de material celulósico. A levedura Pichia pastoris é muito estudada por seu potencial na produção de bioetanol por ser capaz de assimilar pentoses presentes em biomassa celulósica (SHIN et al, 2015).…”

Section: Figura 3 Perfis De Assimilação De Carboidratos Das Levedurasunclassified

Perfis De Assimilação De Carboidratos De Uma Comunidade De Leveduras Associadas Ao Caldo De Cana De Açúcar

Martins¹,

Lima²,

Martins³

2016

Enci Bio

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Bio‐Based Production of Dimethyl Itaconate From Rice Wine Waste‐Derived Itaconic Acid

Joo

You

Shin

et al. 2017

Biotechnology Journal

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Dimethyl itaconate is an important raw material for copolymerization, but it is not synthesized from itaconic acid by organisms. Moreover, Corynebacterium glutamicum is used as an important industrial host for the production of organic acids, but it does not metabolize itaconic acid. Therefore, the biosynthetic route toward dimethyl itaconate from itaconic acid is highly needed. In this study, a biological procedure for dimethyl itaconate production is developed from rice wine waste-derived itaconic acid using the engineered C. glutamicum strain. The first step is to investigate the effect of the co-overexpression of the codon-optimized cis-aconitic acid decarboxylase (CadA*) and a transcriptional regulator of genes involved in acetic acid metabolism (RamA) on itaconic acid production. The second step is to convert itaconic acid into dimethyl itaconate by lipase-catalyzed esterification. The CadA* and RamA-overexpressing CG4 strain increases the itaconic acid concentration under N-starvation with glucose and acetic acid compared with the concentration produced in the base mCGXII medium with glucose. Furthermore, the rice wine waste-derived itaconic acid is successfully converted into dimethyl itaconate using lipase from Rhizomucor miehei and a methanol substrate. This study is the first trial for bio-based production of dimethyl itaconate from rice wine waste-derived itaconic acid.

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Enhanced hydrolysis of lignocellulosic biomass: Bi‐functional enzyme complexes expressed in Pichia pastoris improve bioethanol production from Miscanthus sinensis

Abstract: Research Article Fatty acid hydration activity of a recombinant Escherichia coli-based biocatalyst is improved through targeting the oleate hydratase into the periplasm Sang

Cited by 18 publications

References 28 publications

Design of nanoscale enzyme complexes based on various scaffolding materials for biomass conversion and immobilization

Design of nanoscale enzyme complexes based on various scaffolding materials for biomass conversion and immobilization

Perfis De Assimilação De Carboidratos De Uma Comunidade De Leveduras Associadas Ao Caldo De Cana De Açúcar

Bio‐Based Production of Dimethyl Itaconate From Rice Wine Waste‐Derived Itaconic Acid

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