Bio‐Based Production of Dimethyl Itaconate From Rice Wine Waste‐Derived Itaconic Acid

Abstract

Dimethyl itaconate is an important raw material for copolymerization, but it is not synthesized from itaconic acid by organisms. Moreover, Corynebacterium glutamicum is used as an important industrial host for the production of organic acids, but it does not metabolize itaconic acid. Therefore, the biosynthetic route toward dimethyl itaconate from itaconic acid is highly needed. In this study, a biological procedure for dimethyl itaconate production is developed from rice wine waste-derived itaconic acid using the engineered C. glutamicum strain. The first step is to investigate the effect of the co-overexpression of the codon-optimized cis-aconitic acid decarboxylase (CadA*) and a transcriptional regulator of genes involved in acetic acid metabolism (RamA) on itaconic acid production. The second step is to convert itaconic acid into dimethyl itaconate by lipase-catalyzed esterification. The CadA* and RamA-overexpressing CG4 strain increases the itaconic acid concentration under N-starvation with glucose and acetic acid compared with the concentration produced in the base mCGXII medium with glucose. Furthermore, the rice wine waste-derived itaconic acid is successfully converted into dimethyl itaconate using lipase from Rhizomucor miehei and a methanol substrate. This study is the first trial for bio-based production of dimethyl itaconate from rice wine waste-derived itaconic acid.

Ionic Liquid-Strengthened Immobilized Rhizomucor miehei Lipase for Catalytic Esterification of Itaconic Acid in Aqueous Media

“…Lipases have been reported to preferentially catalyze reactions in organic solvents or at the oil–water interface; they hardly catalyze reactions in the aqueous phase . Thus far, the use of lipases in catalyzing the esterification of IA has received very little attention; only one study has reported the lipase-catalyzed esterification of IA in the aqueous phase, in which the DI yield was only 55.3% . In this study, a higher DI yield was realized.…”

“…Most lipases have poor tolerance to methanol; thus, using water is a better option because water is not only a good solvent for IA but also provides a friendly environment for lipases. In 2017, Joo et al carried out the first trial on the biobased production of DI from rice wine waste-derived IA in aqueous media using Rhizomucor miehei lipase and methanol substrate and achieved the DI yield of only 55.3% …”

Bioconversion of Xylose to Ethylene Glycol and Glycolate in Engineered Corynebacterium glutamicum

“…Recently, metabolically engineered C. glutamicum was grown on alternative carbon sources like xylose 15−17 to produce industrially relevant chemicals. 4,18,19 Previously, ethylene glycol (3.5 g/L) has been produced from glucose at a yield of 0.25 mol/mol (or 0.10 g/g) via the serine biosynthetic pathway using engineered C. glutamicum . 20 In addition, 5.3 g/L glycolate (a yield of 0.18 g/g [0.43 mol/mol]), from a mixture of glucose and acetate, has been produced in engineered C. glutamicum .…”

AFOB Special Issue on Industrial Biotechnology

“…inaquosorum CSB31," [6] the authors screen for a mannanase producer. An extreme alkaline mannanase (MnB31) enzyme was then purified and the biochemical and thermodynamic properties were examined for utilization in various biotechnological processes using different substrates.Moreover, a Research article on the "Bio-based production of dimethyl itaconate from rice wine waste-derived itaconic acid," [7] shows the potential for bio-based production of raw materials for copolymerization from renewable carbon sources. The authors demonstrate that dimethyl itaconate can be produced by engineered C. glutamicum, starting from itaconic acid produced from rice wine waste-derived carbohydrates.…”

“…Moreover, a Research article on the “Bio‐based production of dimethyl itaconate from rice wine waste‐derived itaconic acid,” shows the potential for bio‐based production of raw materials for copolymerization from renewable carbon sources. The authors demonstrate that dimethyl itaconate can be produced by engineered C. glutamicum, starting from itaconic acid produced from rice wine waste‐derived carbohydrates.…”