Enhanced growth and yield of oyster mushroom by growth‐promoting bacteria Glutamicibacter arilaitensis MRC119

Abstract

Promotion of mushroom growth by means of biological agents replacing chemicals is an emerging and highly demanded issue in the sector of mushroom cropping. The present study was aimed to search for a novel bacterium potentially able to enhance mushroom growth and yield. A total of 2165 bacterial isolates purified from different samples were scrutinized through various growth‐promoting attributes. As a consequence of rigorous screening, 26 isolates found exhibiting positive traits of mushroom growth promotion. Thereafter, in response to the cocultivation (fungus and bacteria), a potent bacterial strain was isolated capable to improve significantly the mycelial growth. In cocultivation the highest radial and linear growth rate was 7.6 and 8.1 mm/day on 10th and 11th days, respectively. The fruitbody yields and biological efficiency (BE) of the inoculated sets were 28% and 58% higher than the uninoculated control sets. The bacterium was molecularly identified based on 16S ribosomal RNA sequencing and confirmed as Glutamicibacter arilaitensis MRC119. Therefore, the bioinoculant of the current bacterium can be potentially useful as an ecofriendly substitute stimulating the production of mushroom fruit bodies with improved BE.

Mycelium vs. Fruiting Bodies of Edible Fungi—A Comparison of Metabolites

“…A mycovirus impeded growth and formation of extracellular lignolytic enzymes in P. ostreatus compared with a virus-cured strain [ 89 ], whereas Glutamicibacter arilaitensis , a bacterium belonging to the phylum Actinomycetota and found on some French cheese, promoted growth and yield [ 90 ]. Based on 16 S rRNA gene-terminal restriction fragment length polymorphism, a succession pattern of the microbial community was found, which was thought to protect P. ostreatus against competing fungi [ 91 ].…”

“…They thrive on most of the substrates shown in Table 1 by quickly inducing large enzymatic networks (own experiments, data not shown). As a result, the composition of the fruiting bodies depends on the growth substrate [ 33 , 55 , 57 , 60 , 67 , 72 , 74 ], the strain chosen [ 33 , 60 , 74 , 75 , 76 , 77 , 78 , 80 ], its developmental stage [ 29 , 30 , 31 , 32 , 38 ], and the associated adverse or beneficial microbial community [ 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 ] and is thus variable like no other food. The same applies to the mass production of fungal mycelia.…”

Compost Fungi Allow for Effective Dispersal of Putative PGP Bacteria

“…The isolates belonging to genera Rhodococcus, Agrobacterium, Glutamicibacter and Microbacterium associated with genus Aspergillus and Rhizopus. Microbacterium humic and Microbacterium agarici have previously been isolated from the fungi Agaricus blazei [69], whereas Glutamicibacter arilaitensis has been described to associate with Penicillium [70] and to significantly improve the mycelial growth of edible mushroom Pleurotus florida [71]. Bacteria of the genus Rhodococcus sp.…”

Bacterial Community Composition in the Growth Process of Pleurotus eryngii and Growth-Promoting Abilities of Isolated Bacteria

“…P. putida was the best growth-promoting inoculant among 23 tested bacterial strains that could increase the fruiting-body yield of Agaricus bisporus (Zarenejad et al, 2012). The bioinoculant of Glutamicibacter arilaitensis MRC119 can be potentially used as an eco-friendly substitute improving the fruitbody yields and biological efficiency of oyster mushrooms (Kumari and Naraian, 2021). Pseudomonas fluorescens strains could promote the formation of the primordium, the mycelial growth, and the fruiting body productivity of Pleurotus eryngii (Kim et al, 2008) and Pleurotus ostreatus (Cho et al, 2003).…”