Enhanced functions of osteoblasts on nanophase ceramics

Webster, Thomas J.; Ergun, Celaletdin; Doremus, Robert H.; Siegel, Richard W.; Bizios, Rena

doi:10.1016/s0142-9612(00)00075-2

Cited by 1,215 publications

(769 citation statements)

References 18 publications

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“…All the results above demonstrated that the RN-HA coating material had less negative influence on WJCs' osteoblastic differentiation, and somewhat enhanced WJCs' osteoblastic differentiation. Compared to FM-HA, RN-HA was proved to enhance the osteoblastic differentiation of WJCs, the synthesis of intracellular proteins, the alkaline phosphatase activity and the deposition of calcium-containing mineral, thus enhancing the bonding of orthopedic/dental implants to juxtaposed bone and improving the overall implant efficacy [25,26]. Finally, at the molecular level, OPN, OCN and Runx2 were demonstrated to be expressed during the later extracellular-matrix mineralization [27][28][29][30][31][32][33].…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Guan

Shan

Shi

et al. 2014

Sci. China Life Sci.

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Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton's jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro. Rod-like nano-hydroxyapatite (RN-HA) and flake-like micro-hydroxyapatite (FM-HA) coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group, demonstrating better biocompatibility. Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering.nano-hydroxyapatite, Mg-Zn-Ca alloy, mesenchymal stem cells, Wharton's jelly, biocompatibility Citation:Guan FX, Ma SS, Shi XY, Ma X, Chi LK, Liang S, Cui YB, Wang ZB, Yao N, Guan SK, Yang B. Biocompatibility of nano-hydroxyapatite/ Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro.

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Section: Discussionmentioning

confidence: 99%

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Guan

Shan

Shi

et al. 2014

Sci. China Life Sci.

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“…It is important to point out that Webster et al (Ref 7,14,15) observed higher osteoblast proliferation levels on nanostructured ceramics (including TiO 2 ) when compared to those of conventional ceramics. This higher osteoblast proliferation on nanostructured ceramics was attributed to the higher probability of protein adsorption caused by nano and submicron asperities on the nanomaterialsÕ surfaces.…”

Section: Enhanced Biocompatibility Of Nanomaterialsmentioning

confidence: 95%

HVOF-Sprayed Nano TiO2-HA Coatings Exhibiting Enhanced Biocompatibility

et al. 2009

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HVOF-sprayed nanoBiomedical thermal spray coatings produced via high-velocity oxy-fuel (HVOF) from nanostructured titania (n-TiO 2 ) and 10 wt.% hydroxyapatite (HA) (n-TiO 2 -10wt.%HA) powders have been engineered as possible future alternatives to HA coatings deposited via air plasma spray (APS). This approach was chosen due to (i) the stability of TiO 2 in the human body (i.e., no dissolution) and (ii) bond strength values on Ti-6Al-4V substrates more than two times higher than those of APS HA coatings. To explore the bioperformance of these novel materials and coatings, human mesenchymal stem cells (hMSCs) were cultured from 1 to 21 days on the surface of HVOF-sprayed n-TiO 2 and n-TiO 2 -10 wt.%HA coatings. APS HA coatings and uncoated Ti-6Al-4V substrates were employed as controls. The profiles of the hMSCs were evaluated for (i) cellular proliferation, (ii) biochemical analysis of alkaline phosphatase (ALP) activity, (iii) cytoskeleton organization (fluorescent/confocal microscopy), and (iv) cell/substrate interaction via scanning electron microscopy (SEM). The biochemical analysis indicated that the hMSCs cultured on n-TiO 2 -10 wt.%HA coatings exhibited superior levels of bioactivity than hMSCs cultured on APS HA and pure n-TiO 2 coatings. The cytoskeleton organization demonstrated a higher degree of cellular proliferation on the HVOF-sprayed n-TiO 2 -10wt.%HA coatings when compared to the control coatings. These results are considered promising for engineering improved performance in the next generation of thermally sprayed biomedical coatings.

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“…It should be noted that nanophase ceramics and their composites promoted and supported cell adhesion (and subsequent cell functions such as proliferation [2]) that were previously observed only on material surfaces modified through laborious and lengthy processes that involved immobilization of specially-designed, bioactive, synthetic adhesive peptides [5][6][7]. In contrast, preparation of the nanophase ceramics and their composites was easy, straight-forward and, in terms of time, short (on the order of a few hours).…”

Section: Cell Adhesionmentioning

confidence: 95%

“…The first, statistically-significant evidence of enhanced adhesion of rat calvaria osteoblasts on flat, nanophase (versus microphase/conventional) ceramics (alumina, titania and hydroxylapatite) as a function of decreasing ceramic grain size was provided only a few years ago [1][2]. Most importantly, the enhanced adhesion was specific to osteoblasts, but neither to fibroblasts nor to endothelial cells [3].…”

Section: Cell Adhesionmentioning

confidence: 99%

Mammalian Cell Interactions with Nanophase Materials

Bizios

2004

MRS Proc.

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Adhesion of differentiated mammalian cells from various hard and soft tissues, including adult mesenchymal stem cells is different on nanophase than on microphase/conventional ceramics (such as alumina, titania and hydroxylapatite) as well as on composites of these ceramics with either poly(L-lactic) acid or poly(methyl) methacrylate. Most importantly, nanophase materials promote selective interactions, for example, of osteoblasts but not of fibroblasts. The type, amount and conformation of adsorbed proteins (such as fibronectin, collagen and vitronectin) are key aspects of the underlying mechanism(s) of subsequent cell interactions with nanophase materials. These cellular/molecular results provide evidence that nanophase biomaterials have the potential for improving the efficacy of implants and for promoting neotissue formation pertinent to tissue engineering, regenerative medicine and other clinical applications.

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Enhanced functions of osteoblasts on nanophase ceramics

Cited by 1,215 publications

References 18 publications

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

HVOF-Sprayed Nano TiO2-HA Coatings Exhibiting Enhanced Biocompatibility

Mammalian Cell Interactions with Nanophase Materials

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