Enhanced frequencies of CD14++CD16+, but not CD14+CD16+, peripheral blood monocytes in severe asthmatic patients

Moniuszko, Marcin; Bodzenta-Łukaszyk, Anna; Kowal, Krzysztof; Lenczewska, Danuta; Dąbrowska, Milena

doi:10.1016/j.clim.2008.09.011

Cited by 125 publications

(136 citation statements)

References 22 publications

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“…These are found at low frequency, but they have unique features and expand with cytokine treatment and in inflammation. [11][12][13][14][15][16][17] It has been shown that monocytes can differentiate into DCs in vitro and in vivo. [18][19][20][21][22] Although monocytes, therefore, might be addressed as DC precursors, the panel agreed that these cells still are best called monocytes, because they are not exclusively precursors of DCs but are also the precursors of macrophages.…”

Section: Monocytesmentioning

confidence: 99%

Nomenclature of monocytes and dendritic cells in blood

Ziegler-Heitbrock

Ancuța

Crowe

et al. 2010

Blood

2,048

1,943

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Monocytes and cells of the dendritic cell lineage circulate in blood and eventually migrate into tissue where they further mature and serve various functions, most notably in immune defense. Over recent years these cells have been characterized in detail with the use of cell surface markers and flow cytometry, and subpopulations have been described. The present document proposes a nomenclature for these cells and defines 3 types of monocytes (classical, intermediate, and nonclassical monocytes) and 3 types of dendritic cells (plasmacytoid and 2 types of myeloid dendritic cells) in human and in mouse blood. This classification has been approved by the Nomenclature Committee of the International Union of Immunological Societies, and we are convinced that it will facilitate communication among experts and in the wider scientific community.

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Section: Monocytesmentioning

confidence: 99%

Nomenclature of monocytes and dendritic cells in blood

Ziegler-Heitbrock

Ancuța

Crowe

et al. 2010

Blood

2,048

1,943

View full text Add to dashboard Cite

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“…EFFECTS OF CD14ϩCD16ϩϩ MONOCYTES ON CD14ϩϩCD16Ϫ MONOCYTESously reported ratios (6:4 ratio of CD14ϩϩCD16Ϫ cells to CD14ϩCD16ϩϩ cells) (2,18,(20)(21)(22). The final number of cells per well was 1.2 ϫ 10 5 , using 96-well plates.…”

Section: Uptake Of Acs and Expression Of Hla-dr Cd80mentioning

confidence: 99%

Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes: A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus

Burbano

Vásquez

Rojas

2014

Arthritis & Rheumatology

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Objective. In various chronic inflammatory processes, both the proportion and numbers of monocyte subsets are altered. In systemic lupus erythematosus (SLE), this has not been clearly determined. The monocyte subpopulations in patients with SLE, patients with other autoimmune diseases, and healthy controls were evaluated. The effects of nonclassic monocytes and apoptotic cells (ACs) on the differentiation and function of CD14؉؉CD16؊ monocytes were also studied.Methods. Monocyte subpopulations derived from the blood samples of SLE patients (n ‫؍‬ 88), patients with other autoimmune diseases (n ‫؍‬ 37), and healthy control subjects (n ‫؍‬ 61) were separated by fluorescence-activated cell sorting. To evaluate the effect of CD14؉CD16؉؉ monocytes and ACs on the differentiation of CD14؉؉CD16؊ monocytes, we developed a coculture model of highly purified sorted monocyte subpopulations, which were reconstituted with defined proportions of CD14؉؉CD16؊ and CD14؉CD16؉؉ monocytes in the presence or absence of ACs. After differentiation into macrophages, CD3؉ lymphocytes were added, and the proliferating cells and CD3؉ IFN␥؉ cells were evaluated. A cytokine bead array panel was used to test the coculture supernatants.Results. There was a reduction in CD14؉CD16؉؉ monocytes in patients with active SLE. Monocytes from SLE patients had decreased expression of HLA-DR and decreased ability to bind and phagocytize ACs. In healthy controls, but not SLE patients, treatment with macrophages derived from CD14؉CD16؉؉ monocytes reduced T cell proliferation and proliferating CD3؉IFN␥؉ cells and increased the accumulation of tumor necrosis factor ␣, interleukin-10 (IL-10), and IL-1␤.Conclusion. Our findings show that CD14؉ CD16؉؉ monocytes, a population that is reduced and nonfunctional in SLE patients, have modulatory effects on CD14؉؉CD16؊ monocytes and T cells.

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“…[1][2][3][4][5][6][7][8] However, it is becoming increasingly apparent that further monocyte heterogeneity exists. [9][10][11][12][13][14][15] The new nomenclature that subdivides monocytes into 3 subsets has recently been approved by the Nomenclature Committee of the International Union of Immunologic Societies. 16 Based on this new nomenclature, the major population of human monocytes (ϳ 90%) with high CD14 but no CD16 expression (CD14 ϩϩ CD16 Ϫ ) are now termed classical monocytes, whereas the minor population (ϳ 10%) of human monocytes are further subdivided into the intermediate subset, with low CD16 and high CD14 (CD14 ϩϩ CD16 ϩ ), and the nonclassical subset, with high CD16 but with relatively lower CD14 expression (CD14 ϩ CD16 ϩϩ ).…”

Section: Introductionmentioning

confidence: 99%