Gene expression profiling reveals the defining features of the classical, intermediate, and nonclassical human monocyte subsets

Wong, Kelvin; Tai, June Jing Yi; Wong, William; Hao, Han; Sem, Xiaohui; Yeap, Wei Hseun; Kourilsky, Philippe; Wong, Siew Cheng

doi:10.1182/blood-2010-12-326355

Cited by 903 publications

(1,131 citation statements)

References 49 publications

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“…11,12 Highest TNFa expression was found in non-classical monocytes, consistent with a strong pro-inflammatory potential. HLA-DR and CCR5 were both expressed at highest levels in the intermediate subset, indicating a pronounced role in antigen-presentation as well as chemotactic response to tumor signals, respectively.…”

Section: Discussionmentioning

confidence: 67%

“…Therefore, their role in malignant disease seems ambiguous, as this subset is capable of stimulating tumor angiogenesis on the one hand, but may also exert antitumor effects on the other hand by the production of pro-inflammatory cytokines such as TNF-a, IL-1b and IL-6 and by antigen presentation. 11,24 In the present study with mCRC patients, intermediate monocytes in blood seem to reflect a systemic immunological response to chemotherapy. Thus, their increase in circulation may be induced by a systemic spillover of mediators released by the therapeutically damaged tumor tissue.…”

Section: Discussionmentioning

confidence: 99%

“…10 Recent studies on the gene expression profile of monocyte subsets have suggested a developmental relationship between these populations with gradual changes in surface markers during maturation. 11,12 In accordance, the non-classical subset may represent the most progressed stage of differentiation.…”

Section: Introductionmentioning

confidence: 99%

“…Candidate proteins were again selected based on a former gene expression study showing divergent transcript levels in monocyte subsets for MMP-25 (MT6-MMP) and TIMP-1. 11 Furthermore, the tumor promoter and matrix-degrading protease cathepsin D (CTSD) was investigated, as it has previously been reported to be induced upon monocyte maturation, 19,20 and MMP-14 (MT1-MMP) was included, since it has been specifically connected to monocyte trans-endothelial migration. 21 None of the analyzed proteases showed a preferential expression in intermediate monocytes of CRC patients prior to treatment (Fig.…”

Section: Neoadjuvant Therapy Triggers a Significant Increase In Intermentioning

confidence: 99%

“…11,12 Non-classical monocytes (5%) exhibit a "patrolling" behavior i.e. crawling along vessel walls to screen for integrity 13 and they react strongly to viral signals.…”

Section: Introductionmentioning

confidence: 99%

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Chemotherapy of colorectal liver metastases induces a rapid rise in intermediate blood monocytes which predicts treatment response

et al. 2016

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Neoadjuvant Therapy Triggers a Significant Increase In Intermentioning

confidence: 99%

“…11,12 Non-classical monocytes (5%) exhibit a "patrolling" behavior i.e. crawling along vessel walls to screen for integrity 13 and they react strongly to viral signals.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Chemotherapy of colorectal liver metastases induces a rapid rise in intermediate blood monocytes which predicts treatment response

et al. 2016

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Phagocyte Development

Patel

Yona

2018

Encyclopedia of Life Sciences

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Mature circulating white blood cells can be divided into two lineages: lymphoid and myeloid. The lymphoid lineage consists of T, B, NK and innate lymphoid cells, while the myeloid lineage includes functionally and morphologically distinct cell types including mononuclear phagocytes (monocytes, dendritic cells and macrophages), granulocytes (neutrophils, basophils, mast cells and eosinophils) and platelets. Granulocytes and mononuclear phagocytes are key players at all stages of the immune response from its genesis to its resolution, and come under the banner of phagocytes. Damaged or infected tissue releases chemoattractants that rapidly recruit these phagocytes to the site of injury. Once at the inflamed site, these cells coordinate and carry out immune functions, playing a key role in host defence. The recent past has seen major progress in our understanding of phagocyte developmental biology. Here, we discuss the latest advances in myeloid development, underpinning our current understanding of how these cells are generated and maintained. Key Concepts The myeloid lineage includes functionally and morphologically distinct cell types including mononuclear phagocytes (monocytes, dendritic cells and macrophages) and granulocytes (neutrophils, basophils, mast cells and eosinophils). The majority of tissue resident macrophages are generated before birth and are long‐lived self‐maintained cells throughout adulthood. Dendritic cells prime naïve T cells and are derived from a common precursor in the bone marrow that gives rise exclusively to this family of cells. Monocytes are the principal circulating mononuclear phagocyte. Monocytes develop in the bone marrow from a common monocyte progenitor, once in the circulation classical monocytes have the potential to convert into nonclassical monocytes. Classical monocytes are known to repopulate resident mononuclear phagocyte populations and have potent effector functions during immunity. Granulocyte subsets have a complex morphology with a segmented nucleus and each subset contains granules endowing them with a specific role in host defence. Transcription and growth factors control and dictate the development of phagocytes.

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Association of Elevations of Specific T Cell and Monocyte Subpopulations in Rheumatoid Arthritis With Subclinical Coronary Artery Atherosclerosis

Winchester

Giles

Nativ

et al. 2015

Arthritis & Rheumatology

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Objective Coronary artery disease (CAD) is the leading cause of excess deaths in RA. However, identification of features denoting those with CAD risk is lacking. The composition of circulating mononuclear cell (PBMC) subsets in RA cases differs markedly from healthy controls in extent of T-cell activation with clonal expansion and differentiation to memory effector status and presence of inflammatory monocytes. We sought to evaluate whether elevations in these subpopulations in RA denote those with increased risk for subclinical CAD, as measured by coronary artery calcium (CAC). Methods 72 RA patients underwent cardiac computed tomography to assess CAC. PBMC subsets were determined by multiparameter flow cytometry. Multivariable logistic regression was used to determine the associations between PBMC subpopulations and presence of CAC. Results 33% had CAC and exhibited significant increases of circulating CD4 T cell subsets denoting activation and differentiation to memory effector phenotypes. Analogous increases in CD8 T cell subsets, and intermediate CD14hiCD16+monocytes, were also present compared to those without CAC. The CD4 and CD8 T cell subset increases were highly intercorrelated, while increases in CD14hiCD16+monocytes were independent of the elevated CD4 subsets. After adjustment for relevant confounders, levels of CD4+CD56+CD57+ T cells and CD14hiCD16+monocytes remained associated with the presence of CAC. Conclusions These PBMC subsets are markers for CAC and suggest mechanisms of atherogenesis in RA may operate in part through their increases, raising further questions about the mechanisms underlying the presence of these subset alterations in RA and the potential for shared etiologic pathways between RA and CVD.

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Gene expression profiling reveals the defining features of the classical, intermediate, and nonclassical human monocyte subsets

Cited by 903 publications

References 49 publications

Chemotherapy of colorectal liver metastases induces a rapid rise in intermediate blood monocytes which predicts treatment response

Chemotherapy of colorectal liver metastases induces a rapid rise in intermediate blood monocytes which predicts treatment response

Phagocyte Development

Association of Elevations of Specific T Cell and Monocyte Subpopulations in Rheumatoid Arthritis With Subclinical Coronary Artery Atherosclerosis

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