Enhanced ethanol production from sugarcane molasses by industrially engineered <i>Saccharomyces cerevisiae via</i> replacement of the <i>PHO4</i> gene

Wu, Renzhi; Chen, Dong; Cao, Shuwei; Zhang, Lu; Huang, Jun; Lü, Qi; Chen, Ying; Chen, Xiaoling; Guan, Ni; Wei, Yutuo; Huang, Ribo

doi:10.1039/c9ra08673k

Cited by 52 publications

(23 citation statements)

References 28 publications

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“…Sugarcane molasses (SCM) is the most traditional feedstock for ethanol production in Brazil and India, which is a dark brown liquid obtained as a by-product during sugarrefining process. A good amount of sugar (50-60%) remains in SCM affecting the revenue of the process adversely, which necessitates its sugar content to be converted into ethanol to take full advantage of sugarcane [53,54]. SCM is comprised of 80-85°brix, 44-60% total sugars, 31-34% sucrose, 16-17% reducing sugars, 12.69% ash (wet weight), amino acids, and minerals e.g., 300-12000 ppm potassium, 150-2000 ppm calcium, and 80-3900 ppm magnesium with pH of 5-5.8 [54].…”

Section: Sugary Feedstocksmentioning

confidence: 99%

“…A study was reported for fermentation of SCM containing 390 g/l total sugars with optimum urea (nitrogen source) and inoculum (S. cerevisiae) dosage at 4 and 0.5 g/l, respectively, at 35°C resulting into an ethanol concentration of 87 g/l with a fermentation efficiency of 85.12% [56]. Maiti, Rathore, Srivastava, Shekhawat, and Srivastava [57] investigated another study of fermentation of SCM containing initial total sugars of 216 g/l using 10% (v/v) inoculum size (Zymomonas mobilis 2427) at 31°C, and reported maximum ethanol yield of 59.59 g/l after 44 h. More recently, Wu, Chen, Cao, Lu, Huang, Lu, Chen, Chen, Guan, and Wei [53] studied the SCM fermentation with 70°C Brix value using industrially engineered strain S. cerevisiae MF01-PHO4 with 2 × 10 8 cells/ml, 0.2% (w/w) urea, and 0.02% (w/w) phosphoric acid at 30°C, and reported a maximum ethanol yield of 114.71 g/l after 24 h [53]. Another sugarrich feedstock which has been well studied for 1G ethanol production is sweet sorghum, which provides high ethanol yield per hectare (ha) of cultivation.…”

Section: Sugary Feedstocksmentioning

confidence: 99%

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Production of first- and second-generation ethanol for use in alcohol-based hand sanitizers and disinfectants in India

Hans

Lugani

Chandel

et al. 2021

Biomass Conv. Bioref.

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Emergence of "severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)" causing "COVID-19" or "coronavirus disease 19" as pandemic has got worldwide attention towards hygiene as the first line of defense for the infection control. It is first line of defense not only from COVID-19 but also from other infectious diseases caused by deadly pathogens such as cholera, hepatitis, tuberculosis, polio, etc. Absence of any particular vaccine or treatment let World Health Organization (WHO) recommend to the public to maintain social distancing along with regularly washing their hands with soap, sanitize their hands (where washing is not possible), and disinfect their belongings and buildings to avoid the infection. Out of various formulations available in the market, WHO has recommended alcohol-based hand sanitizers, which mainly comprise of ethanol, isopropyl alcohols, and hydrogen peroxides in different combinations due to their high potential to kill the broad range of pathogens including bacterial, viral, fungal, helminthes, etc. Therefore, alcohol-based sanitizers are in high demand since centuries to prevent infection from pathogenic diseases. Ethanol is the most common and popular alcohol in terms of vanishing wide range of pathogens, convenient to use and its production. Ethanol is produced worldwide and is used in various sectors, e.g., beauty and cosmetics, food and beverages, and as the most demanding gasoline additive. The present review is focused on the ethanol production in India, its diversified applications emphasizing hand sanitizers with discussions on formulation of sanitizer and disinfectants, and viability of lignocellulosic and food grain-based ethanol. The review article also emphasizes on the technological details of 1G and 2G ethanol production, their associated challenges, and inputs for the improved ethanol yields so as to strengthen the supply chain of ethanol in India, and making "Atmanirbhar Bharat" (Self-reliant India) campaign of Indian government successfully viable.

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Section: Sugary Feedstocksmentioning

confidence: 99%

Section: Sugary Feedstocksmentioning

confidence: 99%

Production of first- and second-generation ethanol for use in alcohol-based hand sanitizers and disinfectants in India

Hans

Lugani

Chandel

et al. 2021

Biomass Conv. Bioref.

View full text Add to dashboard Cite

show abstract

“…In a recent study, industrially engineered S. cerevisiae MF01-PHO4 was produced by protoplast formation and pho4 gene replacement, and the mutant strain was observed to be stable for up to 30 generations. An enhanced ethanol yield of 114.71 g/L was achieved with the genetically engineered strain, accounting for 5.30% increase in ethanol yield and 12.5% decrease in fermentation time (Wu et al, 2020). There is no clear evidence on side effects of genetically modified microorganisms on environment; thus, there is still a need to take preventive measures to ensure environmental safety.…”

Section: Strain Development For Improved Bioethanol Fermentationmentioning

confidence: 99%

Recent advances in bioethanol production from lignocelluloses: a comprehensive review with a focus on enzyme engineering and designer biocatalysts

et al. 2020

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Many countries have their biofuel policy programs in place as part of their overall strategy to achieve sustainable development. Among biofuels, bioethanol as a promising alternative to gasoline is of substantial interest. However, there is limited availability of a sufficient quantity of bioethanol to meet demands due to bottlenecks in the present technologies to convert non-edible feedstocks, including lignocelluloses. This review article presents and critically discusses the recent advances in the pretreatment of lignocellulosic biomass, with a focus on the use of green solvents, including ionic liquids and deep eutectic solvents, followed by enzymatic saccharification using auxiliary proteins for the efficient saccharification of pretreated biomass. Different techniques used in strain improvement strategies to develop hyper-producing deregulated lignocellulolytic strains are also compared and discussed. The advanced techniques employed for fermentation of mixed sugars contained in lignocellulosic hydrolysates for maximizing bioethanol production are summarized with an emphasis on pathway and transporters engineering for xylose assimilation. Further, the integration of different steps is suggested and discussed for efficient biomass utilization and improved ethanol yields and productivity.

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“…Saccharomycetes is the biocatalyst of ethanol produced by sugarcane molasses. However, Yeast's optimum fermentative pH is 4.0-4.5 while sugarcane molasses are about 6.2 (Wu et al 2020). Therefore, a large amount of sulfuric acid should be added to reduce the acidity when diluting.…”

Section: Introductionmentioning

confidence: 99%

Bioethanol Production from Sugarcane Molasses by Engineered Strain Lactobacillus Casei E1

Wang

Tian

Liu

et al. 2021

Preprint

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Sugarcane molasses are considered a potential source for bioethanol's commercial production because of its availability and low market price. It contains high concentrations of fermentable sugars that can be directly metabolized by microbial Fermentation. Lactic acid bacteria, especially Lactobacillus casei, have a high potential to be a biocatalyst in ethanol production that they are characterized by strong abilities of carbohydrate metabolism, ethanol synthesis, and high alcohol tolerance. This study aimed to evaluate the feasibility of producing ethanol from sugarcane molasses by Lactobacillus casei used engineering strain L. casei E1 as a starter culture. The effects of environmental factors on the metabolism of L. casei E1 were analyzed by high-performance liquid chromatography (HPLC) system, and the gene expression of key enzymes in carbon source metabolism was detected using quantitative real-time PCR (RT-qPCR). Results showed that the strain could grow well, ferment sugar quickly and produce high yielded ethanol in cane molasses. By fermenting this bacterium anaerobically at 37°C for 36 h incubation in 5 °BX molasses when the fermenter's pH was controlled at 6.0, ethanol yield reached 13.77 g/L, and carbohydrate utilization percentage was 78.60%. RT-qPCR results verified the strain preferentially ferment glucose and fructose of molasses to ethanol at the molecular level. In addition, the metabolism of sugars, especially fructose, would be inhibited by elevating acidity. Our findings support the theoretical basis for exploring Lactic acid bacteria as a starter culture for converting sugarcane molasses into ethanol.

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Enhanced ethanol production from sugarcane molasses by industrially engineered Saccharomyces cerevisiae via replacement of the PHO4 gene

Abstract: Replacement of a novel candidate ethanol fermentation-associated regulatory gene, PHO4, from a fast-growing strain through a novel strategy (SHPERM-bCGHR), is hypothesised to shorten fermentation time and enhance ethanol yield from sugarcane molasses.

Cited by 52 publications

References 28 publications

Production of first- and second-generation ethanol for use in alcohol-based hand sanitizers and disinfectants in India

Production of first- and second-generation ethanol for use in alcohol-based hand sanitizers and disinfectants in India

Recent advances in bioethanol production from lignocelluloses: a comprehensive review with a focus on enzyme engineering and designer biocatalysts

Bioethanol Production from Sugarcane Molasses by Engineered Strain Lactobacillus Casei E1

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