Enhanced endosomal escape by photothermal activation for improved small interfering&amp;nbsp;RNA delivery and antitumor effect

Yang, Xi; Fan, Bo; Gao, Wei; Li, Liping; Li, Tingting; Sun, Jinghua; Peng, Xianjia; Li, Xiaoyan; Wang, Zhenjun; Wang, Binquan; Zhang, Ruiping; Xie, Jun

doi:10.2147/ijn.s161908

Cited by 33 publications

(26 citation statements)

References 30 publications

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“…The achieved siRNA-mediated low-temperature PTT was able to completely suppress tumor growth upon laser irradiation in HeLa tumor-bearing mice model, as a result of the cooperative enhancement of the efficacy of PTT by GT. In a similar way, Yang et al demonstrated the activity of a melanin-poly-l-lysine (M-PLL) polymer as a siRNA vehicle, able to the overcome the endosomal barrier and to deliver into the cytoplasm the functional genetic material [202]. M-PLL nanoparticles exhibited good biocompatibility, biodegradability and siRNA electrostatic-binding capacity with an optimal ratio of 40:1 M-PLL/siRNA.…”

Section: Ptt-gene Therapymentioning

confidence: 95%

Bio-Applications of Multifunctional Melanin Nanoparticles: From Nanomedicine to Nanocosmetics

et al. 2020

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Bioinspired nanomaterials are ideal components for nanomedicine, by virtue of their expected biocompatibility or even complete lack of toxicity. Natural and artificial melanin-based nanoparticles (MNP), including polydopamine nanoparticles (PDA NP), excel for their extraordinary combination of additional optical, electronic, chemical, photophysical, and photochemical properties. Thanks to these features, melanin plays an important multifunctional role in the design of new platforms for nanomedicine where this material works not only as a mechanical support or scaffold, but as an active component for imaging, even multimodal, and simple or synergistic therapy. The number of examples of bio-applications of MNP increased dramatically in the last decade. Here, we review the most recent ones, focusing on the multiplicity of functions that melanin performs in theranostics platforms with increasing complexity. For the sake of clarity, we start analyzing briefly the main properties of melanin and its derivative as well as main natural sources and synthetic methods, moving to imaging application from mono-modal (fluorescence, photoacoustic, and magnetic resonance) to multi-modal, and then to mono-therapy (drug delivery, anti-oxidant, photothermal, and photodynamic), and finally to theranostics and synergistic therapies, including gene- and immuno- in combination to photothermal and photodynamic. Nanomedicine aims not only at the treatment of diseases, but also to their prevention, and melanin in nature performs a protective action, in the form of nanopigment, against UV-Vis radiations and oxidants. With these functions being at the border between nanomedicine and cosmetics nanotechnology, recently examples of applications of artificial MNP in cosmetics are increasing, paving the road to the birth of the new science of nanocosmetics. In the last part of this review, we summarize and discuss these important recent results that establish evidence of the interconnection between nanomedicine and cosmetics nanotechnology.

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Section: Ptt-gene Therapymentioning

confidence: 95%

Bio-Applications of Multifunctional Melanin Nanoparticles: From Nanomedicine to Nanocosmetics

et al. 2020

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“…It has been reported that photothermal activation can be employed to enhance endosomal escape of therapeutic cargos. 47,48 As shown in Figure 2, Au 800 -CTAB-PAA-PEG-AA exhibited a SPR in response to the wavelength of 800 nm. When stimulated with laser irradiation at 808 nm, 2.5 W/cm 2 for 0.5 minutes, the surface temperature of Au 800 -CTAB-PAA-PEG-AA and Au 800 -CTAB-PAA-PEG-AA.EPI was increased to ~55°C and ~65°C, respectively ( Figure S5), and such temperature is sufficient for hyperthermal effect.…”

Section: Formulation and Physicochemical Characterization Of Aunrepimentioning

confidence: 95%

<p>Development of anisamide-targeted PEGylated gold nanorods to deliver epirubicin for chemo-photothermal therapy in tumor-bearing mice</p>

Wang¹,

Pei²,

Cong³

et al. 2019

IJN

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Background Gold nanorods (AuNRs), due to the optical and electronic properties namely the surface plasma resonance, have been developed to achieve the light-mediated photothermal therapy (PTT) for cancer. However, PTT alone may suffer from inefficient tumor killing. Recently, the combination of PTT and chemotherapy has been utilized to achieve synergistic anticancer effects. Methods In this study, AuNRs capped with hexadecyltrimethylammonium bromide (CTAB), poly(acrylic acid) (PAA), and PEGylated anisamide (a ligand known to target the sigma receptor) have been developed to produce a range of negatively charged anisamide-targeted PEGylated AuNRs (namely Au-CTAB-PAA-PEG-AA) for the combination of PTT and chemotherapy (termed as chemo-photothermal therapy [CPTT]). Epirubicin (EPI, an anthracycline drug) was efficiently loaded onto the surface of Au 800 -CTAB-PAA-PEG-AA via the electrostatic interaction forming Au 800 -CTAB-PAA-PEG-AA.EPI complex. Results The resultant complex demonstrated pH-dependent drug release, facilitated nucleus trafficking of EPI, and induced antiproliferative effects in human prostate cancer PC-3 cells. When Au 800 -CTAB-PAA-PEG-AA.EPI complex was further stimulated with desired laser irradiation, the synergistic outcome was evident in PC-3 xenograft mice. Conclusion These results demonstrate a promising strategy for clinical application of CPTT in cancer.

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“…This localization suggests that NPs appear to work in lysosomes, but in some situations, the function of the lysosomes might decrease NP efficacy. For example, some studies have used NPs to deliver siRNA into cancer cells for gene therapy, but the siRNA might be digested in lysosomes [216]. To overcome this problem, several strategies for facilitating endosome escape, such as ion pair formation, the "proton sponge effect", destabilization of the endosome membrane, and the hydrophobic modification of the NPs, have been researched [217].…”

Section: Issues Of Integrin-targeted Npsmentioning

confidence: 99%

Targeting Integrins in Cancer Nanomedicine: Applications in Cancer Diagnosis and Therapy

Opadele

Onodera

et al. 2019

Cancers

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Due to advancements in nanotechnology, the application of nanosized materials (nanomaterials) in cancer diagnostics and therapeutics has become a leading area in cancer research. The decoration of nanomaterial surfaces with biological ligands is a major strategy for directing the actions of nanomaterials specifically to cancer cells. These ligands can bind to specific receptors on the cell surface and enable nanomaterials to actively target cancer cells. Integrins are one of the cell surface receptors that regulate the communication between cells and their microenvironment. Several integrins are overexpressed in many types of cancer cells and the tumor microvasculature and function in the mediation of various cellular events. Therefore, the surface modification of nanomaterials with integrin-specific ligands not only increases their binding affinity to cancer cells but also enhances the cellular uptake of nanomaterials through the intracellular trafficking of integrins. Moreover, the integrin-specific ligands themselves interfere with cancer migration and invasion by interacting with integrins, and this finding provides a novel direction for new treatment approaches in cancer nanomedicine. This article reviews the integrin-specific ligands that have been used in cancer nanomedicine and provides an overview of the recent progress in cancer diagnostics and therapeutic strategies involving the use of integrin-targeted nanomaterials.

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Enhanced endosomal escape by photothermal activation for improved small interfering RNA delivery and antitumor effect

Cited by 33 publications

References 30 publications

Bio-Applications of Multifunctional Melanin Nanoparticles: From Nanomedicine to Nanocosmetics

Bio-Applications of Multifunctional Melanin Nanoparticles: From Nanomedicine to Nanocosmetics

<p>Development of anisamide-targeted PEGylated gold nanorods to deliver epirubicin for chemo-photothermal therapy in tumor-bearing mice</p>

Targeting Integrins in Cancer Nanomedicine: Applications in Cancer Diagnosis and Therapy

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