Enhanced dispersive solid phase extraction assisted by cloud point strategy prior to fluorometric determination of anti-hepatitis C drug velpatasvir in pharmaceutical tablets and body fluids

El-Wekil, Mohamed M.; Ali, Hassan Refat H.; Marzouk, Adel A.; Ali, Ramadan

doi:10.1039/c7ra13719b

Cited by 18 publications

(7 citation statements)

References 22 publications

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“…The obtained data indicated the high precision of the proposed method at the repeatability and intermediate levels as discovered by the small values of RSD (Table ). Also, accuracy and precision were further examined through statistical analysis of the results obtained by the proposed and reported spectrofluorimetric method using Student's t ‐test and variance ratio F ‐test. Results showed no significant differences between the two methods regarding accuracy and precision (Table ).…”

Section: Resultsmentioning

confidence: 99%

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Study on fluorescence properties of HCV antiviral (velpatasvir) and its fluorimetric determination in presence of sofosbuvir; application to stability study and human plasma

et al. 2018

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Velpatasvir (VLP) is a new, oral, direct-acting antiviral with potent inhibitory activity against all hepatitis C virus (HCV) genotypes. A highly sensitive, simple, fast and specific one fluorometric method for determination of VLP in the presence of sofosbuvir was developed and validated. The fluorescence behavior of VLP in different organic solvents was examined and explained. Methanol was concluded to be the best sensitizing reagent. The native fluorescence intensity of VLP was accomplished at 383 nm with 339 nm for excitation wavelength. The impacts of experimental variables included pH, various organized media, and time of stability were examined and optimized. A linear relationship was achieved between the VLP concentration and the fluorescence intensity in a range of 5 to 5 × 10 ng mL with 0.70 and 0.23 ng mL , for quantitation and detection limits respectively. The proposed method was utilized for analyzing of VLP in human plasma and additionally expanded to examine the stability of VLP after its exposure to various stress conditions, like oxidative, alkaline, acidic, UV, daylight and sunlight conditions, according to ICH guidelines. Furthermore, the kinetics of acidic and oxidative degradations of VLP was examined. Moreover, the half-life times of the reaction (t ) and the first-order reaction rate constants were estimated. Finally, a suggestion for the degradation pathway was presented.

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Section: Resultsmentioning

confidence: 99%

“…Only one fluorometric and few liquid chromatographic methods were reported for determination of VLP . To the best of our knowledge, until now, no article has been issued providing stability indicating assays using the spectrofluorimetric technique for the estimation of VLP.…”

Section: Introductionmentioning

confidence: 99%

Study on fluorescence properties of HCV antiviral (velpatasvir) and its fluorimetric determination in presence of sofosbuvir; application to stability study and human plasma

et al. 2018

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“…Although the provided literature reference was very limited, remarkable sensitivity and good accuracy were obtained when applied to real samples, as well as good sorbent reusability, attesting to the high potential of the proposed methods and sorbents ( Table 4 ). An interesting method proposed by El-Wekil et al saw the use of cobalt hydroxide nanoparticles (CHNPs) as sorbents complementary to reduced graphene oxide (rGO) in the dSPE procedure [ 44 ]. CHNP addition inhibited graphene aggregation and therefore ensured the high surface area of the sorbent, as well as decreasing its binding affinity towards the surfactant, polyethylene glycol (PEG) 6000, used in a cloud point extraction (CPE) system, via the formation of rGO-CHNPs hydrogen bonds.…”

Section: Sorbents Based On Carbonmentioning

confidence: 99%

Recent Materials Developed for Dispersive Solid Phase Extraction

Ścigalski¹,

Kosobucki²

2020

Molecules

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Solid phase extraction (SPE) is an analytical procedure developed with the purpose of separating a target analyte from a complex sample matrix prior to quantitative or qualitative determination. The purpose of such treatment is twofold: elimination of matrix constituents that could interfere with the detection process or even damage analytical equipment as well as enriching the analyte in the sample so that it is readily available for detection. Dispersive solid phase extraction (dSPE) is a recent development of the standard SPE technique that is attracting growing attention due to its remarkable simplicity, short extraction time and low requirement for solvent expenditure, accompanied by high effectiveness and wide applicability. This review aims to thoroughly survey recently conducted analytical studies focusing on methods utilizing novel, interesting nanomaterials as dSPE sorbents, as well as known materials that have been only recently successfully applied in dSPE techniques, and evaluate their performance and suitability based on comparison with previously reported analytical procedures.

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“…Pure VLP showed a pH dependent release, where it showed good dissolution up to pH of 2, after which it decreased drastically as shown in Figure 7. This could possibly be due to the low solubility of VLP at higher pH values [40,41,[69][70][71].…”

Section: In-vitro Release Studiesmentioning

confidence: 99%

In-Vitro and In-Vivo Evaluation of Velpatasvir- Loaded Mesoporous Silica Scaffolds. A Prospective Carrier for Drug Bioavailability Enhancement

et al. 2020

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The limited aqueous solubility of many active pharmaceutical ingredients (APIs) is responsible for their poor performance and low drug levels in blood and at target sites. Various approaches have been adopted to tackle this issue. Most recently, mesoporous silica nanoparticles (MSN) have gained attention of pharmaceutical scientists for bio-imaging, bio-sensing, gene delivery, drug solubility enhancement, and controlled and targeted drug release. Here, we have successfully incorporated the poorly water soluble antiviral drug velpatasvir (VLP) in MSN. These spherical particles were 186 nm in diameter with polydispersity index of 0.244. Blank MSN have specific surface area and pore diameter of 602.5 ± 0.7 m2/g and 5.9 nm, respectively, which reduced after successful incorporation of drug. Drug was in amorphous form in synthesized VLP-loaded silica particles (VLP-MSN) with no significant interaction with carrier. Pure VLP showed poor dissolution with progressive increment in pH of dissolution media which could limit its availability in systemic circulation after oral administration. After VLP loading in silica carriers, drug released rapidly over a wide range of pH values, i.e., 1.2 to 6.8, thus indicating an improvement in the solubility profile of VLP. These particles were biocompatible, with an LD50 of 448 µg/mL, and in-vivo pharmacokinetic results demonstrated that VLP-MSN significantly enhanced the bioavailability as compared to pure drug. The above results clearly demonstrate satisfactory in-vitro performance, biocompatibility, non-toxicity and in-vivo bioavailability enhancement with VLP-MSN.

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Enhanced dispersive solid phase extraction assisted by cloud point strategy prior to fluorometric determination of anti-hepatitis C drug velpatasvir in pharmaceutical tablets and body fluids

Abstract: Highly sensitive and selective solid phase extraction method combined with cloud point extraction strategy was developed for analysis of velpatasvir in its pharmaceutical tablets and biological fluids.

Cited by 18 publications

References 22 publications

Study on fluorescence properties of HCV antiviral (velpatasvir) and its fluorimetric determination in presence of sofosbuvir; application to stability study and human plasma

Study on fluorescence properties of HCV antiviral (velpatasvir) and its fluorimetric determination in presence of sofosbuvir; application to stability study and human plasma

Recent Materials Developed for Dispersive Solid Phase Extraction

In-Vitro and In-Vivo Evaluation of Velpatasvir- Loaded Mesoporous Silica Scaffolds. A Prospective Carrier for Drug Bioavailability Enhancement

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