Enhanced differentiation of neural progenitor cells into neurons of the mesencephalic dopaminergic subtype on topographical patterns

Tan, Kenneth K. B.; Tann, Jason Y.; Sathe, Sharvari R.; Goh, S. H.; Ma, Dongliang; Goh, Eyleen L. K.; Yim, Evelyn K.F.

doi:10.1016/j.biomaterials.2014.11.036

Cited by 54 publications

(47 citation statements)

References 51 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differentiation of neocortical cells has shown to benefit from combining ECM proteins such as collagen I and fibronectin [129], but new differentiation protocols might require performing an ECM microarray [130]. Advanced three-dimensional culture surface modification can additionally be used to further the maturation of the desired cell type [87]. …”

Section: Assessing Differentiation Techniquesmentioning

confidence: 99%

Common pitfalls of stem cell differentiation: a guide to improving protocols for neurodegenerative disease models and research

Engel

Do-Ha

Muñoz

et al. 2016

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Induced pluripotent stem cells and embryonic stem cells have revolutionized cellular neuroscience, providing the opportunity to model neurological diseases and test potential therapeutics in a pre-clinical setting. The power of these models has been widely discussed, but the potential pitfalls of stem cell differentiation in this research are less well described. We have analyzed the literature that describes differentiation of human pluripotent stem cells into three neural cell types that are commonly used to study diseases, including forebrain cholinergic neurons for Alzheimer’s disease, midbrain dopaminergic neurons for Parkinson’s disease and cortical astrocytes for neurodegenerative and psychiatric disorders. Published protocols for differentiation vary widely in the reported efficiency of target cell generation. Additionally, characterization of the cells by expression profile and functionality differs between studies and is often insufficient, leading to highly variable protocol outcomes. We have synthesized this information into a simple methodology that can be followed when performing or assessing differentiation techniques. Finally we propose three considerations for future research, including the use of physiological O2 conditions, three-dimensional co-culture systems and microfluidics to control feeding cycles and growth factor gradients. Following these guidelines will help researchers to ensure that robust and meaningful data is generated, enabling the full potential of stem cell differentiation for disease modeling and regenerative medicine.

show abstract

Section: Assessing Differentiation Techniquesmentioning

confidence: 99%

Common pitfalls of stem cell differentiation: a guide to improving protocols for neurodegenerative disease models and research

Engel

Do-Ha

Muñoz

et al. 2016

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

show abstract

“…Recent reports have shown the potential ability of NSCs to differentiate into DA neurons [34,35]. Overexpression of several genes, such as Brn4, TH, glial cell line-derived neurotrophic factor (GDNF), and Lmx1a, may contribute to the maturation and survival of differentiated DA neurons from NSCs [35][36][37].…”

Section: Neural Stem Cells (Nscs)mentioning

confidence: 99%

Clinical and Experimental Cell Therapy in Parkinson’s Disease

Chang¹,

Kim²

2016

Challenges in Parkinson's Disease

View full text Add to dashboard Cite

Parkinson's disease PD , a chronic neurodegenerative disorder, is characterized as a movement disorder with resting tremor, dyskinesia, gait disturbance, etc. The main pathology is based on the progressive loss of dopaminergic neurons in the substantia nigra of the midbrain. These motor symptoms can be treated by dopaminergic drugs, but over time, the drug's effect has less efficacy, and side effects develop such as involuntary movements. As there is no gold standard long-term treatment for this condition, there is a strong need to develop new drugs and therapies. The clinical and experimental findings of successful intrastriatal transplantation of fetal mesencephalic dopaminergic neurons into the brains of patients with PD have been well established. The development of human stem cell technology including embryonic stem ES cells or induced pluripotent stem iPS cells opened a new field called clinical cell therapy, especially for PD. In this chapter, we cover the scientific progress of the clinical and experimental trials of cell therapy for patients with PD. It also contains the recent advances in the clinical application of stem cells including neural stem cells, mesencephalic stem cell, ESC, and iPS cells and unsolved problems in the clinical setting. The combination of gene therapy and gene-manipulated stem cell application in PD therapy will be the most discussed in this area.

show abstract

“…For instance, it has been found that surface nanotopography of biomaterial scaffolds could direct cytoskeletal organization and focal adhesion (FA) formation, which in turn activate downstream cell signaling and regulate cell behavior via cell mechanotransduction pathway . Moreover, some researchers have found that aligned nanogeometry is able to guide neural alignment and polarity, and even provides assistance for neural differentiation of stem cells . Nevertheless, aligned nanogeometry alone is still inefficient and suboptimal for directly directing the differentiation of stem cells.…”

Section: Introductionmentioning

confidence: 99%

Piezoelectric Nanotopography Induced Neuron‐Like Differentiation of Stem Cells

Zhang

Cui

Wang

et al. 2019

Adv Funct Materials

View full text Add to dashboard Cite

The biophysical characteristics of the extracellular matrix, such as nanotopography and bioelectricity, have a profound influence on cell proliferation, adhesion, differentiation, etc. Recognition of the function of a certain biophysical cue and fabrication of biomaterial scaffolds with specific properties would have important implications and significant applications in tissue engineering. Herein, nanotopographic and piezoelectric biomaterials are fabricated and the combination effect of and individual contribution to proliferation, adhesion, and neuron-like differentiation of rat bone marrowderived mesenchymal stem cells (rbMSCs) are clarified via nanotopography and piezoelectricity. Piezoelectric polyvinylidene fluoride with nanostripe array structures is fabricated, which can generate a surface piezoelectric potential up to millivolt by cell movement and traction. The results reveal a more favorable effect on neuron-like differentiation of rbMSCs from the combination of piezoelectricity and nanotopography rather than nanotopography alone, whereas nanotopography can increase cellular adhesion. This research provides a new insight into designing biomaterials for the potential application in neural tissue engineering.

show abstract

Enhanced differentiation of neural progenitor cells into neurons of the mesencephalic dopaminergic subtype on topographical patterns

Cited by 54 publications

References 51 publications

Common pitfalls of stem cell differentiation: a guide to improving protocols for neurodegenerative disease models and research

Common pitfalls of stem cell differentiation: a guide to improving protocols for neurodegenerative disease models and research

Clinical and Experimental Cell Therapy in Parkinson’s Disease

Piezoelectric Nanotopography Induced Neuron‐Like Differentiation of Stem Cells

Contact Info

Product

Resources

About