Piezoelectric Nanotopography Induced Neuron‐Like Differentiation of Stem Cells

Abstract

The biophysical characteristics of the extracellular matrix, such as nanotopography and bioelectricity, have a profound influence on cell proliferation, adhesion, differentiation, etc. Recognition of the function of a certain biophysical cue and fabrication of biomaterial scaffolds with specific properties would have important implications and significant applications in tissue engineering. Herein, nanotopographic and piezoelectric biomaterials are fabricated and the combination effect of and individual contribution to proliferation, adhesion, and neuron-like differentiation of rat bone marrowderived mesenchymal stem cells (rbMSCs) are clarified via nanotopography and piezoelectricity. Piezoelectric polyvinylidene fluoride with nanostripe array structures is fabricated, which can generate a surface piezoelectric potential up to millivolt by cell movement and traction. The results reveal a more favorable effect on neuron-like differentiation of rbMSCs from the combination of piezoelectricity and nanotopography rather than nanotopography alone, whereas nanotopography can increase cellular adhesion. This research provides a new insight into designing biomaterials for the potential application in neural tissue engineering.

Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin‐derived peptide

“…More recently, another study provided direct evidence that the basic activities of living cells created a local electric field on the piezoelectric nanogenerators that can modulate their own cellular behavior without external chemical or physical stimulation (Murillo et al, 2017). Another recent study also proved that PVDF nanostripes could generate a surface piezoelectric potential via the movement and traction of the cells grown on them (Zhang et al, 2019). On the basis of all these previous evidence, it is possible to postulate that the cell‐scale movement of hESCs induces the piezoelectric potential on the VNm–PVDF scaffold, which, in turn, affects their own differentiation without external mechanical stimulation.…”

Mechanical-cellular electrical conversion model reshapes the immune microenvironment of peripheral nerve by modulating neutrophil extracellular traps

“…In our research, P (VDF-TrFE) /rGO/PCL emerges as a promising material for nerve regeneration [55]. Prior studies have underscored the advantages of biomimetic materials in facilitating bioelectrical signal transmission during peripheral nerve regeneration [56][57][58]. An appropriate local voltage potential has the capacity to instigate a conformational shift in the voltage-gated channel, leading to intracellular calcium transients [23].…”

Bioinspired Mild Photothermal Effect-Reinforced Multifunctional Fiber Scaffolds Promote Bone Regeneration

“…Uniaxially aligned pristine PCL nanofibers were prepared as a control group. Compared with disordered orientation structures, highly ordered architecture can promote cell migration by providing topographic cues. − The scanning electron microscopy (SEM) images and the corresponding Fast Fourier Transform (FFT) patterns of the PCL and PCL/BP nanofibers, respectively, indicated the smooth surface of the nanofibers and their ordered array (Figure f,g). The integration of BP NSs had no significant influence on fiber morphology.…”