Enhanced differentiation of human osteoblasts on Ti surfaces pre-treated with human whole blood

Kopf, Brigitte S.; Schipanski, Angela; Rottmar, Markus; Berner, Simon; Maniura‐Weber, Katharina

doi:10.1016/j.actbio.2015.03.022

Cited by 66 publications

(67 citation statements)

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“…Several methods have been developed to obtain different implant surfaces such as plasma spray, grid blasting, acid etching and anodization (1,3,4), which may result on variations of the topography and chemical composition (1,3,5). The implant surface determined by these treatments may affect the protein adsorption, platelet activation and aggregation, fibrin retention (6), cell surface interaction, and cell tissue development at implant/bone interface (1,3,5,7).…”

Section: Introductionmentioning

confidence: 99%

“…SLActive® (Institut Straumann Ag, Basel, Switzerland) was introduced on the market as a superhydrophilic titanium implant surface, which is produced by sandblasting followed by etching using a mixture of HCl and H 2 SO 4 followed by storing in NaCL solution (12). SLActive has been evaluated in vitro (6,12) and in vivo (7,11,12,16). Recently, a new superhydrophilic implant was commercially available, Acqua® (Neodent, Curitiba, PR, Brazil), which is produced by a similar method than SLActive®, resulting in similar microroughness and Implant surfaces in osseointegration contact angle (17).…”

Section: Introductionmentioning

confidence: 99%

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Influence of Implant Surfaces on Osseointegration: A Histomorphometric and Implant Stability Study in Rabbits

et al. 2015

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The aim of this study was to evaluate the stability and osseointegration of implant with different wettability using resonance frequency analysis (RFA) and histomorphometric analysis (bone implant contact, BIC; and bone area fraction occupied, BAFO) after 2 and 4 weeks in rabbit tibiae. Thirty-two Morse taper implants (length 7 mm, diameter 3.5 mm) were divided according to surface characteristics (n=8): Neo, sandblasted and dual acidetched; and Aq, sandblasted followed by dual acid-etched and maintained in an isotonic solution of 0.9% sodium chloride. Sixteen New Zealand rabbits were used. Two implants of each group were installed in the right and left tibiae according to the experimental periods. The RFA (Ostell®) was obtained immediately and after the sacrifice (2 and 4 weeks). The bone/implant blocks were processed for histomorphometric analysis. Data were analyzed using two-way ANOVA followed by Tukey's test and Pearson's correlation for ISQ, BIC and BAFO parameters (p=0.05). No significant effect of implant, period of evaluation or interaction between implant and period of evaluation was found for BIC and BAFO values (p>0.05). Only period of evaluation had significant effect for RFA values at 4 weeks (p=0.001), and at 2 weeks (p<0.001). RFA values were significantly higher at the final period of evaluation compared with those obtained at early periods. There was a significant correlation between BIC values and BAFO values (p=0.009). Both implant surfaces, Aq and Neo, were able to produce similar implant bone integration when normal cortical bone instrumentation was performed. Influence of Implant Surfaces o n O s s e o i n t e g r a t i o n : A Histomorphometric and Implant S t a b i l i t y S t u d y i n R a b b i t s

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of Implant Surfaces on Osseointegration: A Histomorphometric and Implant Stability Study in Rabbits

et al. 2015

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“…114 Fibrin has also been coated on bioinert metallic implants to enhance cell distribution and response and to release growth factors. Kopf et al 115 noted that preincubation of Ti surfaces with whole human blood led to an increased number of attached cells, enhanced ALP and collagen-I protein expression, and promoted mineralization. These improvements in cell behavior were attributed to the creation of a 3D fibrin network on Ti surfaces and/or the release of cytokines that were embedded within the platelets in the fibrin matrix.…”

mentioning

confidence: 99%

“…These improvements in cell behavior were attributed to the creation of a 3D fibrin network on Ti surfaces and/or the release of cytokines that were embedded within the platelets in the fibrin matrix. 115 Spear et al 25 showed that fibrin deposition on porous networks composed of 444 ferromagnetic stainless steel fibers significantly influenced infiltration of human osteoblasts within the scaffolds. For control samples without fibrin, cells with well-spread morphologies were observed only on the metallic fiber surfaces and junctions, while fibrin-containing samples showed cell attachment over and between the fibers.…”

mentioning

confidence: 99%

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

344

255

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Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation. Fibrin has captured the interest of bone tissue engineers due to its excellent biocompatibility, controllable biodegradability, and ability to deliver cells and biomolecules. Fibrin is particularly appealing because its precursors, fibrinogen, and thrombin, which can be derived from the patient’s own blood, enable the fabrication of completely autologous scaffolds. In this article, we highlight the unique properties of fibrin as a scaffolding material to treat bone defects. Moreover, we emphasize its role in bone tissue engineering nanocomposites where approaches further emulate the natural nanostructured features of bone when using fibrin and other nanomaterials. We also review the preparation methods of fibrin glue and then discuss a wide range of fibrin applications in bone tissue engineering. These include the delivery of cells and/or biomolecules to a defect site, distributing cells, and/or growth factors throughout other pre-formed scaffolds and enhancing the physical as well as biological properties of other biomaterials. Thoughts on the future direction of fibrin research for bone tissue engineering are also presented. In the future, the development of fibrin precursors as recombinant proteins will solve problems associated with using multiple or single-donor fibrin glue, and the combination of nanomaterials that allow for the incorporation of biomolecules with fibrin will significantly improve the efficacy of fibrin for numerous bone tissue engineering applications.

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“…Kopf et al, 2015, em pesquisa analisou a interação precoce entre o sangue e a superfície do implante para saber como afetaria o mecanismo de osseointegração, utilizando a coagulação do sangue em superfícies de titânio: microrugosa hidrofóbica e microrugosa hidrofílica com nanoestruturas, bem como os efeitos de sangue humano em um período de pré-incubação nestas duas superfícies sobre o potencial de diferenciação das células ósseas primárias. A pré-incubação de sangue resultou numa rede de fibrina densa por toda a superfície hidrofílica, mas apenas em fragmentos únicos de fibrina e complexos isolados de fibras na superfície hidrofóbica.…”

Section: Propriedades Das Superfíciesunclassified

Avaliação histológica e histomorfométrica da energia superficial e molhabilidade em implantes de titânio grau IV e grau V: estudo experimental em coelhos

Miranda¹

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Enhanced differentiation of human osteoblasts on Ti surfaces pre-treated with human whole blood

Cited by 66 publications

References 68 publications

Influence of Implant Surfaces on Osseointegration: A Histomorphometric and Implant Stability Study in Rabbits

Influence of Implant Surfaces on Osseointegration: A Histomorphometric and Implant Stability Study in Rabbits

A review of fibrin and fibrin composites for bone tissue engineering

Avaliação histológica e histomorfométrica da energia superficial e molhabilidade em implantes de titânio grau IV e grau V: estudo experimental em coelhos

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