Enhanced decolorization of sulfonated azo dye methyl orange by single and mixed bacterial strains AK1, AK2 and VKY1

Masarbo, Ramesh S.; Ismailsab, Mukram; Monisha, T. R.; Nayak, Anand S.; Karegoudar, T. B.

doi:10.1080/10889868.2018.1516612

Cited by 27 publications

(22 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results are consistent with those reported in [ 49 , 76 ]. However, the appearance of a new band at 1247 cm −1 implied the generation of aromatic amines with -C-N vibrations, as highlighted by Masarbo et al [ 79 ] and Baena-Baldiris et al [ 80 ].…”

Section: Resultsmentioning

confidence: 95%

Methyl Orange Biodegradation by Immobilized Consortium Microspheres: Experimental Design Approach, Toxicity Study and Bioaugmentation Potential

Ibrahim

El‐Fakharany

Abu‐Serie

et al. 2022

Biology

View full text Add to dashboard Cite

Methyl orange (MO) is categorized among the recalcitrant and refractory xenobiotics, representing a significant burden in the ecosystem. To clean-up the surrounding environment, advances in microbial degradation have been made. The main objective of this study was to investigate the extent to which an autochthonous consortium immobilized in alginate beads can promote an efficient biodegradation of MO. By employing response surface methodology (RSM), a parametric model explained the interaction of immobilized consortium (Raoultella planticola, Ochrobactrum thiophenivorans, Bacillus flexus and Staphylococcus xylosus) to assimilate 200 mg/L of MO in the presence of 40 g/L of NaCl within 120 h. Physicochemical analysis, including UV-Vis spectroscopy and FTIR, and monitoring of the degrading enzymes (azoreductase, DCIP reductase, NADH reductase, laccase, LiP, MnP, nitrate reductase and tyrosinase) were used to evaluate MO degradation. In addition, the toxicity of MO-degradation products was investigated by means of phytotoxicity and cytotoxicity. Chlorella vulgaris retained its photosynthetic performance (>78%), as shown by the contents of chlorophyll-a, chlorophyll-b and carotenoids. The viability of normal lung and kidney cell lines was recorded to be 90.63% and 99.23%, respectively, upon exposure to MO-metabolic outcomes. These results reflect the non-toxicity of treated samples, implying their utilization in ferti-irrigation applications and industrial cooling systems. Moreover, the immobilized consortium was employed in the bioremediation of MO from artificially contaminated agricultural and industrial effluents, in augmented and non-augmented systems. Bacterial consortium remediated MO by 155 and 128.5 mg/L in augmented systems of agricultural and industrial effluents, respectively, within 144 h, revealing its mutual synergistic interaction with both indigenous microbiotas despite differences in their chemical, physical and microbial contents. These promising results encourage the application of immobilized consortium in bioaugmentation studies using different resources.

show abstract

Section: Resultsmentioning

confidence: 95%

Methyl Orange Biodegradation by Immobilized Consortium Microspheres: Experimental Design Approach, Toxicity Study and Bioaugmentation Potential

Ibrahim

El‐Fakharany

Abu‐Serie

et al. 2022

Biology

View full text Add to dashboard Cite

show abstract

“…Remarkably, the availability of the appropriate type and concentration of carbon/nitrogen sources boosted MB degradation by inducing the expression of oxidoreductive enzymes, which would favor MB decomposition to less complex metabolites as revealed by [ 76 ]. On the contrary, several studies stated the importance of using yeast extract, peptone and glucose for improving degradation efficiently [ 17 , 24 , 77 , 78 ]. Such nutritive ingredients provide redox mediators such as NADH, which assist in energy generation, protein synthesis and subsequent metabolic activity.…”

Section: Discussionmentioning

confidence: 99%

“…By surveying several studies, the phytotoxicity approach was examined via germination test of plant seeds. Phaselus mungo , Oryza sativa [ 67 ], Vigna radiata [ 86 ], Triticum aestivum and Sorghum vulgare [ 17 , 78 ] are the most widely plants used for such bioassay. Herein, microalga C. vulgaris was recruited to assess the influence of MB and its degradation metabolites on its growth and photosynthetic activity.…”

Section: Discussionmentioning

confidence: 99%

Statistical modeling of methylene blue degradation by yeast-bacteria consortium; optimization via agro-industrial waste, immobilization and application in real effluents

et al. 2021

View full text Add to dashboard Cite

The progress in industrialization everyday life has led to the continuous entry of several anthropogenic compounds, including dyes, into surrounding ecosystem causing arduous concerns for human health and biosphere. Therefore, microbial degradation of dyes is considered an eco-efficient and cost-competitive alternative to physicochemical approaches. These degradative biosystems mainly depend on the utilization of nutritive co-substrates such as yeast extract peptone in conjunction with glucose. Herein, a synergestic interaction between strains of mixed-culture consortium consisting of Rhodotorula sp., Raoultella planticola; and Staphylococcus xylosus was recruited in methylene blue (MB) degradation using agro-industrial waste as an economic and nutritive co-substrate. Via statistical means such as Plackett–Burman design and central composite design, the impact of significant nutritional parameters on MB degradation was screened and optimized. Predictive modeling denoted that complete degradation of MB was achieved within 72 h at MB (200 mg/L), NaNO3 (0.525 gm/L), molasses (385 μL/L), pH (7.5) and inoculum size (18%). Assessment of degradative enzymes revealed that intracellular NADH-reductase and DCIP-reductase were key enzymes controlling degradation process by 104.52 ± 1.75 and 274.04 ± 3.37 IU/min/mg protein after 72 h of incubation. In addition, azoreductase, tyrosinase, laccase, nitrate reductase, MnP and LiP also contributed significantly to MB degradation process. Physicochemical monitoring analysis, namely UV−Visible spectrophotometry and FTIR of MB before treatment and degradation byproducts indicated deterioration of azo bond and demethylation. Moreover, the non-toxic nature of degradation byproducts was confirmed by phytotoxicity and cytotoxicity assays. Chlorella vulgaris retained its photosynthetic capability (˃ 85%) as estimated from Chlorophyll-a/b contents compared to ˃ 30% of MB-solution. However, the viability of Wi-38 and Vero cells was estimated to be 90.67% and 99.67%, respectively, upon exposure to MB-metabolites. Furthermore, an eminent employment of consortium either freely-suspended or immobilized in plain distilled water and optimized slurry in a bioaugmentation process was implemented to treat MB in artificially-contaminated municipal wastewater and industrial effluent. The results showed a corporative interaction between the consortium examined and co-existing microbiota; reflecting its compatibility and adaptability with different microbial niches in different effluents with various physicochemical contents.

show abstract

“…Unlike biosorption, even though the dyes have been removed from the wastewater, the absorbate still holds the dye compounds which requires further physio-chemical treatment to reduce it, whereby decolourisation using bacteria solves both problems by eliminating the dye compound and reducing the need for chemical exposure [ 11 ]. Various azo dye decolourisation using bacteria, such as Reactive Black 5 [ 12 ], Black 5 [ 13 ], Methyl Orange [ 14 ], Acid Red [ 15 ], and Green 19 [ 16 ], have been reported before.…”

Section: Introductionmentioning

confidence: 99%

“…To achieve this, various additional carbon sources and a higher concentration of co-substrate were introduced. During the last ten years, the attention has shifted towards isolating microorganisms with the ability to decolourise azo dyes with complete mineralisation ability [ 14 ]. The focus changed mainly because degraded metabolites of azo dye were found to be toxic [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Biodecolourisation of Reactive Red 120 as a Sole Carbon Source by a Bacterial Consortium—Toxicity Assessment and Statistical Optimisation

Manogaran

Yasid

Othman

et al. 2021

IJERPH

View full text Add to dashboard Cite

The application of microorganisms in azo dye remediation has gained significant attention, leading to various published studies reporting different methods for obtaining the best dye decolouriser. This paper investigates and compares the role of methods and media used in obtaining a bacterial consortium capable of decolourising azo dye as the sole carbon source, which is extremely rare to find. It was demonstrated that a prolonged acclimation under low substrate availability successfully isolated a novel consortium capable of utilising Reactive Red 120 dye as a sole carbon source in aerobic conditions. This consortium, known as JR3, consists of Pseudomonas aeruginosa strain MM01, Enterobacter sp. strain MM05 and Serratia marcescens strain MM06. Decolourised metabolites of consortium JR3 showed an improvement in mung bean’s seed germination and shoot and root length. One-factor-at-time optimisation characterisation showed maximal of 82.9% decolourisation at 0.7 g/L ammonium sulphate, pH 8, 35 °C, and RR120 concentrations of 200 ppm. Decolourisation modelling utilising response surface methodology (RSM) successfully improved decolourisation even more. RSM resulted in maximal decolourisation of 92.79% using 0.645 g/L ammonium sulphate, pH 8.29, 34.5 °C and 200 ppm RR120.

show abstract

Enhanced decolorization of sulfonated azo dye methyl orange by single and mixed bacterial strains AK1, AK2 and VKY1

Cited by 27 publications

References 36 publications

Methyl Orange Biodegradation by Immobilized Consortium Microspheres: Experimental Design Approach, Toxicity Study and Bioaugmentation Potential

Methyl Orange Biodegradation by Immobilized Consortium Microspheres: Experimental Design Approach, Toxicity Study and Bioaugmentation Potential

Statistical modeling of methylene blue degradation by yeast-bacteria consortium; optimization via agro-industrial waste, immobilization and application in real effluents

Biodecolourisation of Reactive Red 120 as a Sole Carbon Source by a Bacterial Consortium—Toxicity Assessment and Statistical Optimisation

Contact Info

Product

Resources

About