Enhanced conjugated linoleic acid and biogas production after ruminal fermentation with Piper betle L. supplementation

Abstract

<jats:p>ABSTRACT: Piper betle L. is edible plant richer in polyphenols that might improve feed utilization in rumen diet. The objective of the present study was to investigate the effect of various Piper betle L. powder (PL) doses on in vitro rumen microorganisms, ruminal biogas and fermentation end-product production, and biohydrogenation including lipolysis-isomerization. The completely randomized design used five levels of PL supplementation (0, 25, 50, 75 and 100 mg DM) incubated with 400 mg of a basal substrate of Pangola hay and concentrate (50:50). The matrix compounds (g/kg DM) of 0.27 catechin, 0.11 rutin, 3.48 quercetin, 0.41 apigenin, 0.04 myricetin, 0.27 kaempferol, 0.76 eugenol and 0.22 caryophyllene derived from PL altered the fermentation pattern, with an increase in degradable nutrients and total volatile fatty acids and acetogenesis without shifting pH during fermentation. These values promoted in vitro gas production, with higher carbon dioxide and lower methane production. Although, hydrogen recovery from lipolysis-isomerization in biohydrogenation was limited, PL successfully promoted stearic acid (C18:0) accumulation by changing the biohydrogenation pathway of fatty acids, causing more C18:1 trans-11 rather than C18:2 trans-11, cis-15. Consequently, this resulted in more conjugated linoleic acid (CLA) cis-9, trans-11, CLA trans-10, cis-12 and CLA trans-11, cis-13. Enhanced PL supply increased total bacteria and fungal zoospores due to a reduction in rumen protozoa. In conclusion, our results demonstrated that PL is a feed additive with potential for ruminants, promising improved ruminal fermentation and biohydrogenation, while reducing methane production.</jats:p>

Silage Fermentation Quality, Anthocyanin Stability, and in vitro Rumen Fermentation Characteristic of Ferrous Sulfate Heptahydrate-Treated Black Cane (Saccharum sinensis R.)

“…Consistent with our findings, Hosoda et al ( 43 ) and Tian et al ( 2 ) revealed that ruminal fluid acetic acid increased in response to anthocyanin-rich maize feeding in ruminant animals. This might be explained by anthocyanins' role in bacterial population regulation, as shown by shifting VFA production and other fermentation gases such as ruminal biogas methane and carbon dioxide, as well as an alternate hydrogen sink ( 2 , 29 , 30 ). According to a recent study, optimizing VFAs production might be accomplished by matching the needs of the rumen host to the needs of the rumen microbiome and the availability of fermentable substrate in rumen fluids ( 48 ).…”

Treatment of Rice Stubble with Pleurotus ostreatus and Urea Improves the Growth Performance in Slow-Growing Goats

“…Rumen fluid was collected (~500 mL) on the next day following the period of feces collection with the use of a stomach tube connected to a manual pump [ 14 , 15 , 16 ]. The rumen fluid samples were taken ~30 min before the morning meal and 2 and 4 h after the morning feeding.…”

Empirical Evaluation and Prediction of Protein Requirements for Maintenance and Growth of 18–24 Months Old Thai Swamp Buffaloes

“…Additionally, there was a shift in VFAs produced in the rumen toward more propionate with corresponding to reduce in acetate and remain in butyrate ( Table 4 ). The acetate portion decreased when the propionate increased, indicating that the dietary CP in the present study altered the fermentable carbohydrate to be a major substrate for acetate fraction; however, a higher propionate suggested that propionate-producing bacteria was dominant to synthesize fermentable carbohydrate more propionate in gluconeogenesis by the pentose phosphate pathway produces a nicotinamide adenine dinucleotide phosphate [ 18 , 49 , 50 ]. These occurrences might relate to the reduction of NDF digestion ( Table 3 ).…”

“…The observed NDF digestion playing a role in the VFA-producing substrate is line with data by Vorlaphim, et al [ 51 ], who reported no changes for propionate portion in rumen fluids at 0 h, 2 h, and 4 h after feedings when NDF intake (g/d) was similar. It, thus, can be suggested that this declining availability of fermentable carbohydrate might relate to decrease acetate portion as the first major VFA absorbed from the rumen to have somewhat distinctive metabolic shift [ 28 , 49 , 50 ]. This achievement was similar with the previous study [ 32 ] and was expected in the present study because of the altered carbohydrate digestion.…”