Enhanced cell uptake of fluorescent drug-loaded nanoparticles via an implantable photothermal fibrous patch for more effective cancer cell killing

Abstract

Great efforts have been devoted to effective delivery of therapeutics into cells for cancer therapy. The exploration of nanoparticle based drug delivery systems (DDSs) faces daunting challenges in low efficacy of intracellular delivery. Herein, a localized drug delivery device consisting of photoluminescent mesoporous silica nanoparticles (PLMSNs) and photothermal fibrous matrix was investigated. Specifically, PLMSNs modified with a pH-sensitive polydopamine (PDA) ‘gatekeeper’ served as a doxorubicin (DOX) carrier and could release DOX once the PLMSNs were up-taken by the cancer cells. The PLMSNs were electrostatically assembled on the surface of electrospun biodegradable poly(ε-caprolactone)/gelatin fibrous mesh incorporated with photothermal carbon nanoparticles (CNPs), leading to an implantable patch used as localized delivery platform. Comparing to free particulate DDSs, this implantable composite patch device was found to significantly enable superior cell up-taking effect and consequently enhance in-vitro therapeutic efficacy against tumor cells. Namely, under near infrared irradiation, the photothermal effect of CNPs in the implantable patch weakens the electrostatic interaction between the PLMSNs and poly(ε-caprolactone)/gelatin/CNP fibrous mesh, resulting in the controlled release of the PLMSNs and subsequent internalization into the tumor cells for more effective cancer cell killing. This implantable therapeutic device may therefore inspire another way of developing localized cancer therapy.

Spun Biotextiles in Tissue Engineering and Biomolecules Delivery Systems

“…Compared to drug administrations in the free form, the implant was seen to significantly enable a superior cell uptake effect, thus increasing the drug efficacy against tumor cells by responding to under-near-infrared irradiation. The photothermal effect of the carbon nanotubes weakened the electrostatic interaction between the photoluminescent nanocarriers and the PCL/gelatin nanofibers, resulting in the controlled release and, subsequently, internalization of DOX for a more effective cancer cell killing action [243].…”

Delivery of Nanoparticle-Based Radiosensitizers for Radiotherapy Applications

“…Some researchers believe that NPs can accumulate within tumors due to the EPR effect while avoiding most normal cells [41,56]. Passively targeted NPs can be specifically designed as a result of the EPR effect [20,37,[57][58][59]. Although the passive targeting technique is clinically accepted, it has numerous limitations, such as inefficient NP/drug diffusion, the random nature of NP circulation in the blood prior to reaching the tumor environment, and the fact that some tumors do not display an EPR effect, and the penetrability of vessels are unlikely to be uniform in a single tumor [53,55,60,61].…”

Gold nanorod-assembled ZnGa2O4:Cr nanofibers for LED-amplified gene silencing in cancer cells

“…The synthesis of ZGOC nanofibers was performed using via the modified a modified protocol based on previous studies reported in the literature. [52][53][54] The electrospinning precursor was prepared by a standard sol-gel approach. Ga(NO3)3•xH2O (0.475 g), Zn(NO3)3•6H2O (0.279 g) and Cr(NO3)3•9H2O (0.002 g) were added to 2 mL deionized water 6 mL ethanol and 3 mL N,N-dimethylformamide (DMF) and mixed well by stirring for 1 h. Subsequently, 0.7 g PVP was added and stirred for further 3 h to obtain a homogeneous solution.…”