Enhanced camptothecin production induced by elicitors in the cell suspension cultures of Ophiorrhiza mungos Linn.

Deepthi, S.; Satheeshkumar, K.

doi:10.1007/s11240-015-0908-y

Cited by 75 publications

(20 citation statements)

References 48 publications

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“…The degree of induction in the mixed culture was reported to depend on the types and concentrations of mixed strains and on the cell growth stage while mixing 23 . The three-day culture medium of the S .…”

Section: Resultsmentioning

confidence: 99%

Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production

Sun

Wang

et al. 2017

Sci Rep

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Mixing cultures induces the biosynthesis of laccase in mixed cells, produces signal molecules, and regulates the production of mixed-cell metabolites. The fungal strain, which promotes laccase production, has been isolated and screened from the host bamboos of endophytic fungi and identified as Phoma sp. BZJ6. When the culture medium is mainly composed of soluble starch, yeast extract, and Phoma sp., the laccase output can reach 4,680 U/L. Nitric oxide (NO) and reactive oxygen species (ROS) were found to promote the regulation of laccase synthesis. Plasma membrane NAD(P)H oxidase inhibitors and NO-specific quenchers can inhibit not only the accumulation of ROS induced and NO synthesis but also the biosynthesis of laccase. The results indicate that the accumulation of superoxide anion radical (O2 −) and hydrogen peroxide (H2O2) induced by the mixed culture was partially dependent on NO. The mixed culture can also reduce the biomass, increase the synthesis of total phenolics and flavonoids, and enhance the activity of phenylalanine ammonia-lyase and chalcone isomerase. This phenomenon is probably the result of the activated phenylpropanoids–flavonoid pathway. Results confirmed that the mixture culture is advantageous for laccase production and revealed that NO, O2 −, and H2O2 are necessary signal molecules to induce laccase synthesis.

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Section: Resultsmentioning

confidence: 99%

Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production

Sun

Wang

et al. 2017

Sci Rep

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“…In the study, CPT production by the original parent strain OmF3 under the uninduced condition was found to be CPT is a quinoline alkaloid with potent anticancer activity isolated originally from C. acuminata by Wall et al 34 This has also been detected from other plant resources like Ophiorrhiza species. 35,36 Due to the pharmaceutical importance of CPT, alternative sources for its production is significant. At the same time, several endophytes such as Entrophospora infrequens, Neurospora sp., Trichoderma sp., Fusarium solani, and so on have already been characterized for CPT production.…”

Section: Enhancement Of Cpt Production By Np Supplementationmentioning

confidence: 99%

Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos

Aswani

Jasim

Vishnu

et al. 2020

Biotechnology Progress

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In the study, endophytic fungi isolated from Ophiorrhiza mungos were screened for camptothecin (CPT) biosynthetic potential by high performance liquid chromatography (HPLC). Among the 16 fungi screened, OmF3, OmF4, and OmF6 were identified to synthesize CPT. Further LC-MS analysis also showed the presence of CPT specific m/z of 349 for the extracts from OmF3, OmF4, and OmF6. However, the fragmentation masses with m/z of 320, 305, 277 and 220 specific to the CPT could be identified only for the OmF3 and OmF4. These CPT producing fungi were further identified as Meyerozyma sp. OmF3 and Talaromyces sp. OmF4. The cultures of these two fungi were then supplemented with nanoparticles and analyzed for the quantitative enhancement of CPT production by LC-MS/MS. From the result, Meyerozyma sp. OmF3 was found to produce 947.3 ± 12.66 μg/L CPT, when supplemented with 1 μg/ mL zinc oxide nanoparticles and the same for uninduced parental strain OmF3 was only 1.77 ± 0.13 μg/L. At the same time, Talaromyces sp. OmF4 showed the highest production of 28.97 ± 0.37 μg/L of CPT when cultured with 10 μg/mL silver nanoparticles and the same for uninduced strain was 1.19 ± 0.24 μg/L. The observed quantitative enhancement of fungal CPT production is highly interesting as it is a rapid and cost effective method. The study is remarkable due to the identification of novel fungal sources for CPT production and its enhancement by nanoparticle supplementation.

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“…CSC [78] Ophiorrhiza alata Craib HRC [79] Ophiorrhiza mungos Linn. CSC [80] Ophiorrhiza prostata D. Don ARC [81] Ophiorrhiza pumila Champ. ex Benth.…”

Section: Monoterpene Indole Alkaloidunclassified

Production of Plant Secondary Metabolites by Using Biotechnological Tools

Gonçalves¹,

Romano²

2018

Secondary Metabolites - Sources and Applications

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Plants are a remarkable source of high-value secondary metabolites with applications in various domains. Plant cell and tissue culture techniques appear as environmentally friendly alternatives for the production of secondary metabolites when natural supply is limited or chemical synthesis is unviable. In this chapter, the main advantages of using plant cell and tissue culture techniques for the production of plant secondary metabolites are presented as well as the different biotechnological approaches available to improve their production. In addition, the production of anticancer compounds (camptothecin, podophyllotoxin, taxol, vinblastibe, and vincristine) and metabolites from Lamiaceae spp. (phenolics as rosmarinic acid) were selected as examples to be highlighted. The study reviewed shows that undifferentiated cells are the preferred culture system used for the production of high-value secondary metabolites in vitro although there are many examples reporting the production in differentiated tissues particularly in hairy roots. Efforts have been made to scale up the production, and several strategies have been successfully applied to increase the production yields at the laboratorial scale. Nevertheless, there are only few examples of plant secondary metabolites production at commercial level, and further in-depth studies are still required.

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Enhanced camptothecin production induced by elicitors in the cell suspension cultures of Ophiorrhiza mungos Linn.

Cited by 75 publications

References 48 publications

Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production

Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production

Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos

Production of Plant Secondary Metabolites by Using Biotechnological Tools

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