Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos

Abstract

In the study, endophytic fungi isolated from Ophiorrhiza mungos were screened for camptothecin (CPT) biosynthetic potential by high performance liquid chromatography (HPLC). Among the 16 fungi screened, OmF3, OmF4, and OmF6 were identified to synthesize CPT. Further LC-MS analysis also showed the presence of CPT specific m/z of 349 for the extracts from OmF3, OmF4, and OmF6. However, the fragmentation masses with m/z of 320, 305, 277 and 220 specific to the CPT could be identified only for the OmF3 and OmF4. These CPT producing fungi were further identified as Meyerozyma sp. OmF3 and Talaromyces sp. OmF4. The cultures of these two fungi were then supplemented with nanoparticles and analyzed for the quantitative enhancement of CPT production by LC-MS/MS. From the result, Meyerozyma sp. OmF3 was found to produce 947.3 ± 12.66 μg/L CPT, when supplemented with 1 μg/ mL zinc oxide nanoparticles and the same for uninduced parental strain OmF3 was only 1.77 ± 0.13 μg/L. At the same time, Talaromyces sp. OmF4 showed the highest production of 28.97 ± 0.37 μg/L of CPT when cultured with 10 μg/mL silver nanoparticles and the same for uninduced strain was 1.19 ± 0.24 μg/L. The observed quantitative enhancement of fungal CPT production is highly interesting as it is a rapid and cost effective method. The study is remarkable due to the identification of novel fungal sources for CPT production and its enhancement by nanoparticle supplementation.

Isolation, anticancer potency, and camptothecin—producing ability of endophytic fungi isolated from Ixora chinensis

“…The ability of the I3R2 strain to produce CPT was reconfirmed by NMR analysis. CPT was isolated and verified with the chemical shifts of 1 H and 13 C NMR spectra (Figure S5, Figure S6). 1 S3).…”

“…CPT was extracted using the protocol mentioned above and purified via HPLC system (Agilent HPLC 1290 Infinity II) by collecting the fraction of the peak as CPT. Finally, 4 mg of purified CPT (90%) was dissolved in CDCl 3 for 1 H and 13 C structural elucidation. 1D NMR spectra were recorded on a Bruker Avance 600 MHz spectrometer (Bruker Biospin, Rheinstetten, Germany) using TMS (tetramethylsilane) as an internal standard.…”

“…OmF3 and Talaromyces sp. OmF4, 13 Diaporthe sp. F18, 14 Aspergillus flavus ER, and Aspergillus terreus.…”

Isolation, anticancer potency and camptothecin – producing ability of endophytic fungi isolated from Ixora chinensis

“…OmF3 and Talaromyces sp. OmF4 (Aswani et al 2020), Diaporthe sp. F18 (Degambada et al 2023), Aspergillus avus ER, and Aspergillus terreus (El-Sayed et al 2022).…”