Enhanced bone tissue regeneration by antibacterial and osteoinductive silica-HACC-zein composite scaffolds loaded with rhBMP-2

Zhou, Panyu; Xia, Yan; Cheng, Xiaoqing; Wang, Panfeng; Xie, Yang; Xu, Shuogui

doi:10.1016/j.biomaterials.2014.09.009

Cited by 86 publications

(59 citation statements)

References 48 publications

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“…Bone morphogenetic proteins particularly the BMP-II are one of the osteoinductive compounds. However, such growth factors are expensive, require special preservative condition before surgical application, and have non-negligible limitations and side effects [38][39][40]. Pre-differentiated stem cells or osteoblastic cells can also induce bone formation; however, they are associated with significant limitations in the clinical situation.…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

et al. 2015

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Treatment of large bone defects (LBDs) is technically demanding. Tissue engineering is an option. A bioactive graft may be produced by combining tissue scaffolds and healing promotive factors in order to accelerate bone repair. We investigated the role of Simvastatin (Sim)-embedded porous Gelapin (Gel) scaffold on experimental bone healing. At first, the effectiveness of different concentrations of Gel and Sim powders was investigated in an experimentally induced femoral hole model in rabbits (n = 6) for 30 days. Then bone bioactive grafts were produced by combination of the effective concentrations of Gel, Sim, and Genipin. The bioimplants were subcutaneously tested in a rabbit model (n = 9) to determine their biocompatibility and biodegradability for 10-30 days. Finally, a large radial bone defect model was produced in rabbits (n = 20), and the bioimplants were inserted in the defects. The untreated and autograft-treated bone defects were served as controls. The animals were euthanized after 30 and 60 days of bone injury. The bone samples were evaluated by radiography, three-dimensional CT scan, bone densitometry, histopathology, and nano-indentation. At a concentration of 5 mg/hole, Sim closed the femoral bone holes after 30 days, while in the defect, autograft, and Gel groups, the holes were open. Both the Gel and Gel-Sim scaffolds were biocompatible and biodegradable. Subcutaneously, the Gel-Sim scaffold was replaced with the newly regenerated ectopic bone after 30 days. After implantation of the Gel-Sim scaffold in the radial bone defects, the scaffold was completely replaced with new woven bone after 30 days which was then matured and remodeled into a cortical bone after 60 days. Sixty days after bone injury, the Gel-Sim-treated defects had significantly higher bone volume, matrix mineralization, elastic modulus, and contact hardness when compared to the controls. The Gel-Sim scaffold may be a suitable option in managing LBDs.

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Section: Discussionmentioning

confidence: 99%

Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

et al. 2015

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“…However, the cell toxicity of QCS used to fabricate tissue engineering scaffolds remains a serious issue. Although Zhou's [36,37] reports used QCS with a degree of substitution (DS) of 18% to fabricate antibacterial scaffold for bone tissue engineering, the relatively low DS of chitosan might have a limited water solubility to prepare injectable hydrogel scaffolds [35].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and conductive injectable hydrogels based on quaternized chitosan-graft-polyaniline/oxidized dextran for tissue engineering

et al. 2015

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“…[3][4][5][6][7][8] Ideally, the scaffolds used for bone tissue engineering should have a three-dimensional, highly porous structure with an interconnected pore network. All these geometrical features facilitate cell migration and tissue ingrowth and are important for neovascularization, affecting directly the flow of nutrients and metabolic waste.…”

Section: Introductionmentioning

confidence: 99%

Effect of bioactive glass particles on osteogenic differentiation of adipose‐derived mesenchymal stem cells seeded on lactide and caprolactone based scaffolds

Larrañaga

Alonso‐Varona

Palomares

et al. 2015

J Biomedical Materials Res

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Incorporation of bioactive glass (BG) particles to synthetic polymer scaffolds is a promising strategy to improve the bioactivity of bioinert materials and to stimulate specific cell responses. In this study, the influence of incorporating BG particles to lactide and caprolactone based porous scaffolds on osteogenic differentiation of adipose-derived stem cells (ASCs) was analyzed. Accordingly, ASCs were seeded on poly(L-lactide) (PLLA), poly(ε-caprolactone) (PCL), or poly(L-lactide-co-ε-caprolactone) (PLCL) scaffolds containing 15 vol % of BG particles in two culture conditions: standard versus osteogenic culture medium. In standard culture medium, incorporation of BG to a PLLA scaffold increased the ALP activity with respect to its unfilled counterpart (ca. 1.2- and a 1.6-fold increase over 7 and 14 days, respectively). Moreover, in all the studied polymers the incorporation of BG induced a slightly higher production of mineralized matrix by ASCs, but the differences observed were not statistically significant. In the osteogenic medium, the effect of BG was masked by the effect of osteogenic supplements in the long-term. However, in the short-term (day 7), BG particles induced an early ALP activity of predifferentiated osteoblasts on PLLA and PCL scaffolds and higher matrix mineralization on PCL scaffolds. In summary, the addition of BG particles to PLLA and PCL scaffolds sustains ASC osteogenic differentiation, facilitates mineralization and induces the formation of a hydroxyapatite layer on the surface of the polymer scaffolds.

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Enhanced bone tissue regeneration by antibacterial and osteoinductive silica-HACC-zein composite scaffolds loaded with rhBMP-2

Cited by 86 publications

References 48 publications

Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

Antibacterial and conductive injectable hydrogels based on quaternized chitosan-graft-polyaniline/oxidized dextran for tissue engineering

Effect of bioactive glass particles on osteogenic differentiation of adipose‐derived mesenchymal stem cells seeded on lactide and caprolactone based scaffolds

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