Enhanced bone apposition around biofunctionalized sandblasted and acid‐etched titanium implant surfaces

Abstract

Microrough titanium (Ti) surfaces of dental implants have demonstrated more rapid and greater bone apposition when compared with machined Ti surfaces. However, further enhancement of osteoblastic activity and bone apposition by bio-functionalizing the implant surface with a monomolecular adsorbed layer of a co-polymer - i.e., poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) and its derivatives (PLL-g-PEG/PEG-peptide) - has never been investigated. The aim of the present study was to examine early bone apposition to a modified sandblasted and acid-etched (SLA) surface coated with an Arg-Gly-Asp (RGD)-peptide-modified polymer (PLL-g-PEG/PEG-RGD) in the maxillae of miniature pigs, and to compare it with the standard SLA surface. Test and control implants had the same microrough topography (SLA), but differed in their surface chemistry (polymer coatings). The following surfaces were examined histomorphometrically: (i) control - SLA without coating; (ii) (PLL-g-PEG); (iii) (PLL-g-PEG/PEG-RDG) (RDG, Arg-Asp-Gly); and (iv) (PLL-g-PEG/PEG-RGD). At 2 weeks, RGD-coated implants demonstrated significantly higher percentages of bone-to-implant contact as compared with controls (61.68% vs. 43.62%; P < 0.001). It can be concluded that the (PLL-g-PEG/PEG-RGD) coatings may promote enhanced bone apposition during the early stages of bone regeneration.

Osteogenic activity of titanium surfaces with hierarchical micro/nano-structures obtained by hydrofluoric acid treatment

“…Additive methods typically use templates fabricated through titanium plasma-spraying, electron-beam lithography, molecular self-assembly, and sol-gel transformation techniques, among others. These methods provide more complex micro-/nano-structures, and drugs can be coated onto the surface (such as the molecular self-assembly method) 40 and sub-10 nm length scale structure can be achieved (such as electron-beam lithography). 41 Subtractive methods typically use templates fabricated through blasting, peroxidation (H 2 O 2 ) or acid oxidation, and acid etching.…”

Research Progress on the Biochemical Modification of Titanium Implant Surface

“…It was concluded that organic coating of machined screw implant surfaces providing binding sites for integrin receptors can enhance bone implant contact and periimplant bone formation. In addition, Germanier et al [46] compared RGD peptide polymer modified implant surfaces with sandblasted and acid-etched implant surfaces placed in the maxillae of miniature pigs, and confirmed that the functionalization may promote enhanced bone apposition during the early stages of bone regeneration. Despite these highly positive results, another in vivo study found that the presence of the RGD sequence did not improve the adhesion on Ti.…”

Nanofeatured Titanium Surfaces for Dental Implantology: Biological Effects, Biocompatibility, and Safety

“…Molecular selfassembly allows for the deposition of a self-assembled monolayer onto a substrate and subsequent exposure of an active terminal group that promotes specific biological functions such as the adhesion of protein and cells [94] or bone deposition [95]. This technique sounds promising because it allows obtaining a selective adhesion to the surface.…”