Enhanced biosynthesis of triterpenoids in Centella asiatica hairy root culture by precursor feeding and elicitation

Baek, Seungeun; Ho, Thanh-Tam; Lee, Hyoshin; Jung, Gooyoung; Kim, Young Eun; Jeong, Cheol-Seung; Park, So‐Young

doi:10.1007/s11816-019-00573-w

Cited by 32 publications

(16 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Elicitation with 100 µM MJ influenced silymarin pigment accumulation in cell culture of Silybum marimum after 3 days of treatment [68]. In another report, elicitation with 4 µM MJ for 2 weeks resulted in a 6.5-fold enhancement of solasodine content (9.33 mg g −1 DW) than un-elicited hairy root cultures of Solanum trilobatum [69]. In addition, increasing of withanolide derivatives accumulation (14-fold) was achieved in 40-day-old hairy roots elicited with 15 µM MJ for 4 h exposure time [70].…”

Section: Application Of Mj For Enhancing Secondary Metabolite In In Vmentioning

confidence: 93%

Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures

Murthy

Park

2020

IJMS

Self Cite

166

View full text Add to dashboard Cite

Recently, plant secondary metabolites are considered as important sources of pharmaceuticals, food additives, flavours, cosmetics, and other industrial products. The accumulation of secondary metabolites in plant cell and organ cultures often occurs when cultures are subjected to varied kinds of stresses including elicitors or signal molecules. Application of exogenous jasmonic acid (JA) and methyl jasmonate (MJ) is responsible for the induction of reactive oxygen species (ROS) and subsequent defence mechanisms in cultured cells and organs. It is also responsible for the induction of signal transduction, the expression of many defence genes followed by the accumulation of secondary metabolites. In this review, the application of exogenous MJ elicitation strategies on the induction of defence mechanism and secondary metabolite accumulation in cell and organ cultures is introduced and discussed. The information presented here is useful for efficient large-scale production of plant secondary metabolites by the plant cell and organ cultures.

show abstract

Section: Application Of Mj For Enhancing Secondary Metabolite In In Vmentioning

confidence: 93%

Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures

Murthy

Park

2020

IJMS

Self Cite

166

View full text Add to dashboard Cite

show abstract

“…Affordable and high production of secondary metabolites, high genetic stability, and rapid accumulation and growth of biomass are only some of the merits of hairy root cultures [ 46 ]. A larger scale and more profitable process can also be achieved by the cultivation of hairy roots in bioreactors [ 56 ]. Sirikantaramas et al [ 57 ] isolated Δ 9 -tetrahydrocannabinolic acid synthase (THCAS) from cannabis leaves and cloned its cDNA.…”

Section: In Vitro Culture In Cannabismentioning

confidence: 99%

Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis

Hesami

Baiton

Alizadeh

et al. 2021

IJMS

View full text Add to dashboard Cite

For a long time, Cannabis sativa has been used for therapeutic and industrial purposes. Due to its increasing demand in medicine, recreation, and industry, there is a dire need to apply new biotechnological tools to introduce new genotypes with desirable traits and enhanced secondary metabolite production. Micropropagation, conservation, cell suspension culture, hairy root culture, polyploidy manipulation, and Agrobacterium-mediated gene transformation have been studied and used in cannabis. However, some obstacles such as the low rate of transgenic plant regeneration and low efficiency of secondary metabolite production in hairy root culture and cell suspension culture have restricted the application of these approaches in cannabis. In the current review, in vitro culture and genetic engineering methods in cannabis along with other promising techniques such as morphogenic genes, new computational approaches, clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR/Cas9-equipped Agrobacterium-mediated genome editing, and hairy root culture, that can help improve gene transformation and plant regeneration, as well as enhance secondary metabolite production, have been highlighted and discussed.

show abstract

“…Advancements in biotechnological methods of enhancement of biosynthesis of specialized metabolites in plant in vitro cultures throughout the last couple decades have enabled research to combine increased productivity with minimized effects exerted on general metabolism. Thus, recent reports concerning the triterpenoid saponin productivity in various cell, tissue, and organ cultures of plants described in this review often do not contain the analysis of sterol content [ 84 , 85 , 86 , 87 ], since the phenomenon of their competition with triterpenoid pathway can be neglected after successful application of a suitable strategy.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Phytosterol and Triterpenoid Production in Plant Hairy Root Cultures—Simultaneous Stimulation or Competition?

Rogowska

Szakiel

2021

Plants

View full text Add to dashboard Cite

Plant in vitro cultures, including hairy roots, can be applied for controlled production of valuable natural products, such as triterpenoids and sterols. These compounds originate from the common precursor squalene. Sterols and triterpenoids distinctly differ in their functions, and the 2,3-oxidosqualene cyclization step is often regarded as a branch point between primary and secondary (more aptly: general and specialized) metabolism. Considering the crucial role of phytosterols as membrane constituents, it has been postulated that unconstrained biosynthesis of triterpenoids can occur when sterol formation is already satisfied, and these compounds are no longer needed for cell growth and division. This hypothesis seems to follow directly the growth-defense trade-off plant dilemma. In this review, we present some examples illustrating the specific interplay between the two divergent pathways for sterol and triterpenoid biosynthesis appearing in root cultures. These studies were significant for revealing the steps of the biosynthetic pathway, understanding the role of particular enzymes, and discovering the possibility of gene regulation. Currently, hairy roots of many plant species can be considered not only as an efficient tool for production of phytochemicals, but also as suitable experimental models for investigations on regulatory mechanisms of plant metabolism.

show abstract

Enhanced biosynthesis of triterpenoids in Centella asiatica hairy root culture by precursor feeding and elicitation

Cited by 32 publications

References 36 publications

Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures

Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures

Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis

Enhancement of Phytosterol and Triterpenoid Production in Plant Hairy Root Cultures—Simultaneous Stimulation or Competition?

Contact Info

Product

Resources

About