Enhanced biosurfactant production by Bacillus subtilis SPB1 using developed fed-batch fermentation: effects of glucose levels and feeding systems

Bouassida, Mouna; Mnif, Inès; Ghribi, Dhouha

doi:10.1007/s00449-022-02839-0

Cited by 6 publications

(2 citation statements)

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“…The types and quantities of biosurfactants produced by microbes are closely related to their growth environment [ 56 ] and nutritional conditions [ 57 ]. Therefore, exploring the growth environment and nutritional conditions of microorganisms is also a popular research topic for expanding the yield of target biological surfactants [ 58 ]. Currently, the majority of biosurfactant studies concentrate on complex bioactive agents like glycolipids and lipopeptides, whereas there are fewer studies on neutral lipids, phospholipids, and fatty acids as surfactants.…”

Section: Biosurfactants: Properties Synthesis and Classificationmentioning

confidence: 99%

Application of Biosurfactants in Medical Sciences

Wang,

An,

Cao

et al. 2024

Molecules

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Biosurfactants derived from microorganisms have attracted widespread attention in scientific research due to their unique surface activity, low toxicity, biodegradability, antibacterial properties, and stability under extreme conditions. Biosurfactants are widely used in many fields, such as medicine, agriculture, and environmental protection. Therefore, this review aims to comprehensively review and analyze the various applications of biosurfactants in the medical field. The central roles of biosurfactants in crucial medical areas are explored, like drug delivery, induction of tumor cell differentiation or death, treating bacterial and viral effects, healing wounds, and immune regulation. Moreover, a new outlook is introduced on optimizing the capabilities of biosurfactants through modification and gene recombination for better use in medicine. The current research challenges and future research directions are described, aiming to provide valuable insights for continuous study of biosurfactants in medicine.

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Section: Biosurfactants: Properties Synthesis and Classificationmentioning

confidence: 99%

Application of Biosurfactants in Medical Sciences

Wang,

An,

Cao

et al. 2024

Molecules

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“…The most critical aspect to be considered is the possibility of obtaining a VJ that has the same The fed-batch strategy had already been identified as an effective method for improving surfactin production [63]. Bouassida et al showed that by exponential feeding in fed-batch cultivation, it is possible to increase the amount of lipopeptide produced by more than twofold compared to batch fermentation using B. subtilis SPB1 cultured in a glucose-based medium [64]; a similar result was found in the present study, in fact, with respect to the quantities of lipopeptides found in the flask cultivation (1.30 g/L of surfactin and 11.00 mg/L of fengycin produced in 25% VJ medium). B. subtilis BMV9 produced 2.77 g/L of surfactin and 27.53 mg/L of fengycin only 16 h after the start of fermentation in the bioreactor.…”

Section: Fed-batch Bioreactor Cultivationmentioning

confidence: 99%

Fed-Batch Bioreactor Cultivation of Bacillus subtilis Using Vegetable Juice as an Alternative Carbon Source for Lipopeptides Production: A Shift towards a Circular Bioeconomy

Gugel,

Vahidinasab,

Benatto Perino

et al. 2024

Fermentation

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In a scenario of increasing alarm about food waste due to rapid urbanization, population growth and lifestyle changes, this study aims to explore the valorization of waste from the retail sector as potential substrates for the biotechnological production of biosurfactants. With a perspective of increasingly contributing to the realization of the circular bioeconomy, a vegetable juice, derived from unsold fruits and vegetables, as a carbon source was used to produce lipopeptides such as surfactin and fengycin. The results from the shake flask cultivations revealed that different concentrations of vegetable juice could effectively serve as carbon sources and that the fed-batch bioreactor cultivation strategy allowed the yields of lipopeptides to be significantly increased. In particular, the product/substrate yield of 0.09 g/g for surfactin and 0.85 mg/g for fengycin was obtained with maximum concentrations of 2.77 g/L and 27.53 mg/L after 16 h, respectively. To conclude, this study provides the successful fed-batch cultivation of B. subtilis using waste product as the carbon source to produce secondary metabolites. Therefore, the consumption of agricultural product wastes might be a promising source for producing valuable metabolites which have promising application potential to be used in several fields of biological controls of fungal diseases.

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