Enhanced biostability and cellular uptake of zinc oxide nanocrystals shielded with a phospholipid bilayer

Dumontel, Bianca; Canta, Marta; Engelke, Hanna; Chiodoni, Angelica; Racca, Luisa; Ancona, Andrea; Limongi, Tania; Canavese, Giancarlo; Cauda, Valentina

doi:10.1039/c7tb02229h

Cited by 78 publications

(89 citation statements)

References 53 publications

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“…The reaction path is based on the hydrolysis of the zinc precursors (zinc acetate dihydrate) due to the presence of sodium hydroxide as the base in methanol. The as-synthesized ZnO were then functionalized with amino-propyl groups with a post-grafting approach using 3-(AminoPropyl)-TriEthoxySilane (APTES) at 10 mol% with respect to the molar amount of ZnO, as in Dumontel et al (2017) and Garino et al (2019a).…”

Section: Zno-nh 2 Ncs Synthesis and Functionalizationmentioning

confidence: 99%

The Synergistic Effect of Nanocrystals Combined With Ultrasound in the Generation of Reactive Oxygen Species for Biomedical Applications

Vighetto

Ancona

Racca

et al. 2019

Front. Bioeng. Biotechnol.

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Reactive oxygen species (ROS) effects on living cells and tissues is multifaceted and their level or dose can considerably affect cell proliferation and viability. It is therefore necessary understand their role also designing ways able to regulate their amount inside cells, i.e., using engineered nanomaterials with either antioxidant properties or, for cancer therapy applications, capable to induce oxidative stress and cell death, through tunable ROS production. In this paper, we report on the use of single-crystalline zinc oxide (ZnO) round-shaped nanoparticles, yet ZnO nanocrystals (NCs) functionalized with amino-propyl groups (ZnO-NH 2 NCs), combined with pulsed ultrasound (US). We show the synergistic effects produced by NC-assisted US which are able to produce different amount of ROS, as a result of inertial cavitation under the pulsed US exposure. Using Passive Cavitation Detection (PCD) and Electron Paramagnetic Resonance (EPR) spectroscopy, we systematically study which are the key parameters, monitoring, and influencing the amount of generated ROS measuring their concentration in water media and comparing all the results with pure water batches. We thus propose a ROS generation mechanism based on the selective application of US to the ZnO nanocrystals in water solutions. Ultrasound B-mode imaging is also applied, proving in respect to pure water, the enhanced ecographic signal generation of the aqueous solution containing ZnO-NH 2 NCs when exposed to pulsed ultrasound. Furthermore, to evaluate the applicability of ZnO-NH 2 NCs in the biomedical field, the ROS generation is studied by interposing different tissue mimicking materials, like phantoms and ex vivo tissues, between the US transducer and the sample well. As a whole, we clearly proof the enhanced capability to produce ROS and to control their amount when using ZnO-NH 2 NCs in combination with pulsed ultrasound anticipating their applicability in the fields of biology and health care.

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Section: Zno-nh 2 Ncs Synthesis and Functionalizationmentioning

confidence: 99%

The Synergistic Effect of Nanocrystals Combined With Ultrasound in the Generation of Reactive Oxygen Species for Biomedical Applications

Vighetto

Ancona

Racca

et al. 2019

Front. Bioeng. Biotechnol.

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“…In previous researches, different methods were followed to decrease agglomerations, and improve dispersion, stability, and biocompatibility of the nanomaterials [12,13], such as an organic coating or addition of a capping agent (polyethylene glycol (PEG), l-dopa, poly-n-vinyl-2-pyrrolidone (PVP) and others [14,15]. However, the cytotoxic effects of capping agents or coatings have also been reported [16].…”

Section: Introductionmentioning

confidence: 99%

Lipid-coated ZnO nanoparticles synthesis, characterization and cytotoxicity studies in cancer cell

et al. 2020

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ZnO nanoparticles are widely used in biological, chemical, and medical fields, but their toxicity impedes their wide application. In this study, pristine ZnO NPs (~ 7 nm; ~ 18 nm; ~ 49 nm) and lipid-coated ZnO NPs (~ 13 nm; ~ 22 nm; ~ 52 nm) with different morphologies were prepared by chemical method and characterized by TEM, XRD, HRTEM, FTIR, and DLS. Our results showed that the lipid-coated ZnO NPs (~ 13 nm; ~ 22 nm; ~ 52 nm) groups improved the colloidal stability, prevented the aggregation and dissolution of nanocrystal particles in the solution, inhibited the dissolution of ZnO NPs into Zn 2+ cations, and reduced cytotoxicity more efficiently than the pristine ZnO NPs (~ 7 nm; ~ 18 nm; ~ 49 nm). Compared to the lipid-coated ZnO NPs, pristine ZnO NPs (~ 7 nm; ~ 18 nm; ~ 49 nm) could dose-dependently destroy the cells at low concentrations. At the same concentration, ZnO NPs (~ 7 nm) exhibited the highest cytotoxicity. These results could provide a basis for the toxicological study of the nanoparticles and direct future investigations for preventing strong aggregation, reducing the toxic effects of lipid-bilayer and promoting the uptake of nanoparticles by HeLa cells efficiently.

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“…ZnO NPs (15 nm) decorated with both (1,2-dioleoyl-sn-glycero-3-phosphocholine) (DOPC) and amine-propyl chains exhibited a positive charge in water. However, DOPC-ZnO NPs were internalized at a significantly higher level than were NH 2 -ZnO NPs in HeLa cells, a distinction that may be due to the difference in positive charge (Dumontel et al, 2017). Moreover, the uptake level that is affected by surface charge may vary with cell type.…”

Section: Surface Chargementioning

confidence: 89%

Cytotoxicity-Related Bioeffects Induced by Nanoparticles: The Role of Surface Chemistry

Sun

Jiang

Li-jun

et al. 2019

Front. Bioeng. Biotechnol.

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Nanoparticles (NPs) are widely used in a variety of fields, including those related to consumer products, architecture, energy, and biomedicine. Once they enter the human body, NPs contact proteins in the blood and interact with cells in organs, which may induce cytotoxicity. Among the various factors of NP surface chemistry, surface charges, hydrophobicity levels and combinatorial decorations are found to play key roles inregulating typical cytotoxicity-related bioeffects, including protein binding, cellular uptake, oxidative stress, autophagy, inflammation, and apoptosis. In this review, we summarize the recent progress made in directing the levels and molecular pathways of these cytotoxicity-related effects by the purposeful design of NP surface charge, hydrophobicity, and combinatorial decorations.

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Enhanced biostability and cellular uptake of zinc oxide nanocrystals shielded with a phospholipid bilayer

Abstract: The surface chemistry and charge of zinc oxide nanocrystals influence their behaviour in biological fluids. A novel lipid bilayer assembly is developed to shield ZnO nanocrystals improving their stability and cell internalization.

Cited by 78 publications

References 53 publications

The Synergistic Effect of Nanocrystals Combined With Ultrasound in the Generation of Reactive Oxygen Species for Biomedical Applications

The Synergistic Effect of Nanocrystals Combined With Ultrasound in the Generation of Reactive Oxygen Species for Biomedical Applications

Lipid-coated ZnO nanoparticles synthesis, characterization and cytotoxicity studies in cancer cell

Cytotoxicity-Related Bioeffects Induced by Nanoparticles: The Role of Surface Chemistry

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