Enhanced biodegradation of total polycyclic aromatic hydrocarbons (TPAHs) by marine halotolerant Achromobacter xylosoxidans using Triton X-100 and β-cyclodextrin – A microcosm approach

Dave, Bharti P.; Ghevariya, Chirag M.; Bhatt, Jwalant K.; Dudhagara, Dushyant R.; Rajpara, Rahul K.

doi:10.1016/j.marpolbul.2013.12.027

Cited by 50 publications

(11 citation statements)

References 16 publications

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“…P. fluorescens and R. dairenensis also produced greater degradation of aromatics. A. xylosoxidans , which originated from marine environments, have been shown to be able to efficiently degrade a wide range of low/high molecular weight PAHs as well as many xenobiotic substrates including toluidine isomers, endosulfan, p‐toluenesulfonate, chlorocatechol, 2,4‐dichlorophenol, organophosphorous pesticide, etc . P. fluorescens has also been reported as the important genus of PAH utilizers .…”

Section: Resultsmentioning

confidence: 99%

“…A. xylosoxidans, which originated from marine environments, have been shown to be able to efficiently degrade a wide range of low/high molecular weight PAHs as well as many xenobiotic substrates including toluidine isomers, endosulfan, p-toluenesulfonate, chlorocatechol, 2,4-dichlorophenol, organophosphorous pesticide, etc. 30,31 P. fluorescens has also been reported as the important genus of PAH utilizers. 32,33 Cerqueira et al 6 demonstrated that B. megaterium strain BB06 could degrade 89.0% and 39.6% of aliphatic and aromatic fractions, respectively, in oily sludge.…”

Section: Oily Sludge Biodegradation In Biometer Flasksmentioning

confidence: 99%

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Enhanced bioremediation of oily sludge using co‐culture of specific bacterial and yeast strains

Xin-guo

Liu

2014

J of Chemical Tech & Biotech

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BACKGROUND Considerable volumes of petrochemical oily sludge can be generated from oil production and processing activities. Total petroleum hydrocarbons (TPH) contained in oily sludge are detrimental to human health and the environment. Thus, oily sludge is classified as a priority environmental pollutant by the US Environmental Protection Agency and it must be treated properly before discharge into the environment. RESULTS In this study, a functional microbial consortium consisting of Bacillus subtilis, Bacillus megaterium, Achromobacter xylosoxidans, Pseudomonas fluorescens, Candida tropicalis and Rhodotorula dairenensis, isolated from petroleum‐contaminated soil, was applied for bioremediation of petrochemical oily sludge in a shake flask and oily sludge microcosm study. These strains utilized the oily sludge as sole source of carbon and energy. The oily sludge contained 21.5% (w/w, dry weight basis) of total petrol hydrocarbons (TPH). Results showed that the microbial consortium exhibited an excellent oily sludge degradation capacity, reducing 92.5% of aliphatics, 79.3% of aromatics, 48.6% of NSO‐containing compounds, 31.8% of asphaltenes and 80.6% of TPH, by the end of the 1‐year microcosm experiment. In contrast, biodegradation rates of these fractions were 83.3%, 17.5%, 8.4%, 4.7% and 56.1%, respectively, in the non‐inoculated treatment. These strains survived well and adapted to the oil sludge environment throughout the incubation period. Dehydrogenase and polyphenoloxidase activity of the oily sludge was remarkably promoted by inoculation of the microbial consortium. CONCLUSION The results indicated that the microbial consortium has the potential to be used in bioremediation of petrochemical oily sludge polluted environments. © 2014 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Section: Oily Sludge Biodegradation In Biometer Flasksmentioning

confidence: 99%

Enhanced bioremediation of oily sludge using co‐culture of specific bacterial and yeast strains

Xin-guo

Liu

2014

J of Chemical Tech & Biotech

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“…and Achromobacter sp. (Arulazhagan and Vasudevan, 2011; Dave et al, 2014). On the other hand, we found that B. safensis PHA3 degraded more actively the pyrene and benzo(e)pyrene, the two first members of HMW-PAHs.…”

Section: Discussionmentioning

confidence: 99%

Biochemical, Molecular, and Transcriptional Highlights of the Biosynthesis of an Effective Biosurfactant Produced by Bacillus safensis PHA3, a Petroleum-Dwelling Bacteria

2017

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Petroleum crude oil (PCO)-dwelling microorganisms have exceptional biological capabilities to tolerate the toxicity of petroleum contaminants and are therefore promising emulsifier and/or degraders of PCO. This study describes a set of PCO-inhabiting bacterial species, one of which, identified as Bacillus safensis PHA3, produces an efficient biosurfactant which was characterized as a glycolipid. Fourier transform infrared spectrometer, nuclear magnetic resonance, Thin layer chromatography, HPLC, and GC-MS analysis of the purified biosurfactant revealed that the extracted molecule under investigation is likely a mannolipid molecule with a hydrophilic part as mannose and a hydrophobic part as hexadecanoic acid (C16:0). The data reveal that: (i) PHA3 is a potential producer of biosurfactant (9.8 ± 0.5 mg mL-1); (ii) pre-adding 0.15% of the purified glycolipid enhanced the degradation of PCO by approximately 2.5-fold; (iii) the highest emulsifying activity of biosurfactant was found against the PCO and the lowest was against the naphthalene; (iv) the optimal PCO-emulsifying activity was found at 30–60°C, pH 8 and a high salinity. An orthologous gene encodes a putative β-diglucosyldiacylglycerol synthase (β-DGS) was identified in PHA3 and its transcripts were significantly up-regulated by exogenous PAHs, i.e., pyrene and benzo(e)pyrene but much less by mid-chain n-alkanes (ALKs) and fatty acids. Subsequently, the accumulation of β-DGS transcripts coincided with an optimal growth of bacteria and a maximal accumulation of the biosurfactant. Of particular interest, we found that PHA3 actively catalyzed the degradation of PAHs notably the pyrene and benzo(e)pyrene but was much less effective in the mono-terminal oxidation of ALKs. Such characteristics make Bacillus safensis PHA3 a promising model for enhanced microbial oil recovery and environmental remediation.

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“…The rate of petrol degradation was maximum (31.9%) at the end of 15 days interval period by Achromobacter. Recently, Achromobacter sp has been reported to utilize polycyclic aromatic hydrocarbons [46][47][48][49][50]. PAH degradation by Pseudomonas strains is well characterized [51].…”

Section: Discussionmentioning

confidence: 99%

Characterization and Evaluation of Polycyclic Aromatic Hydrocarbon (PAH) Degrading Bacteria Isolated from Oil Contaminated Soil

Simaria¹,

Pant²

2015

Appli Micro Open Access

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Polycyclic aromatic hydrocarbons (PAHs) are common environmental pollutants and biodegradation using microorganisms is the preferred and major route of PAH removal from contaminated environments. This study investigated the bacterial degradation of petrol and diesel in liquid media that were isolated from oil contaminated soils by enrichment technique. The isolates could use petrol and diesel as their sole carbon and energy source in Bushnell Hass Mineral Salts (BHMS) medium at 2% (v/v) concentration. A total of eight isolates were selected and characterized by using a variety of phenotypic and morphologic properties. Two isolates each showed highest growth in petrol and diesel containing media during screening were selected and characterized using 16S RNA sequencing. Molecular identification of the isolates assigned them to Achromobacter sp. and Pseudomonas aeruginosa. The selected isolates degraded petrol and diesel up to 31.9% and 34.4% respectively. This study indicates that the contaminated soil samples contain a diverse population of PAH-degrading bacteria and the use of Achromobacter sp. and Pseduomonas aeruginosa has the potential for bioremediation of PAH contaminated sites.

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Enhanced biodegradation of total polycyclic aromatic hydrocarbons (TPAHs) by marine halotolerant Achromobacter xylosoxidans using Triton X-100 and β-cyclodextrin – A microcosm approach

Cited by 50 publications

References 16 publications

Enhanced bioremediation of oily sludge using co‐culture of specific bacterial and yeast strains

Enhanced bioremediation of oily sludge using co‐culture of specific bacterial and yeast strains

Biochemical, Molecular, and Transcriptional Highlights of the Biosynthesis of an Effective Biosurfactant Produced by Bacillus safensis PHA3, a Petroleum-Dwelling Bacteria

Characterization and Evaluation of Polycyclic Aromatic Hydrocarbon (PAH) Degrading Bacteria Isolated from Oil Contaminated Soil

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