Enhanced biodegradation of organic waste treated by environmental fungal isolates with higher cellulolytic potential

Awais, Muhammad; Fatma, Shabih; Naveed, Ahad; Batool, Uzma; Shehzad, Qamar; Hameed, Amir

doi:10.1007/s13399-021-01932-w

Cited by 3 publications

(3 citation statements)

References 61 publications

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“…Then, five grams of each dry, milled (0.5–1 cm) substrate were mixed with 2.5 g yeast extract/L deionised water, with the moisture level maintained at 60%. Next, a fungal spore suspension (10 6 cells/mL) was aseptically inoculated in flasks (5%; w/v of the substrate) and incubated at 30 °C for 5 days [ 91 ].…”

Section: Methodsmentioning

confidence: 99%

Application of a novel biological-nanoparticle pretreatment to Oscillatoria acuminata biomass and coculture dark fermentation for improving hydrogen production

et al. 2023

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Background Energy is the basis and assurance for a world's stable development; however, as traditional non-renewable energy sources deplete, the development and study of renewable clean energy have emerged. Using microalgae as a carbon source for anaerobic bacteria to generate biohydrogen is a clean energy generation system that both local and global peers see as promising. Results Klebsiella pneumonia, Enterobacter cloacae, and their coculture were used to synthesize biohydrogen using Oscillatoria acuminata biomass via dark fermentation. The total carbohydrate content in O. acuminata was 237.39 mg/L. To enhance the content of fermentable reducing sugars, thermochemical, biological, and biological with magnesium zinc ferrite nanoparticles (Mg-Zn Fe2O4-NPs) pretreatments were applied. Crude hydrolytic enzymes extracted from Trichoderma harzianum of biological pretreatment were enhanced by Mg-Zn Fe2O4-NPs and significantly increased reducing sugars (230.48 mg/g) four times than thermochemical pretreatment (45.34 mg/g). K. pneumonia demonstrated a greater accumulated hydrogen level (1022 mLH2/L) than E. cloacae (813 mLH2/L), while their coculture showed superior results (1520 mLH2/L) and shortened the production time to 48 h instead of 72 h in single culture pretreatments. Biological pretreatment + Mg-Zn Fe2O4 NPs using coculture significantly stimulated hydrogen yield (3254 mLH2/L), hydrogen efficiency)216.9 mL H2/g reducing sugar( and hydrogen production rate (67.7 mL/L/h) to the maximum among all pretreatments. Conclusion These results confirm the effectiveness of biological treatments + Mg-Zn Fe2O4-NPs and coculture dark fermentation in upregulating biohydrogen production.

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Section: Methodsmentioning

confidence: 99%

Application of a novel biological-nanoparticle pretreatment to Oscillatoria acuminata biomass and coculture dark fermentation for improving hydrogen production

et al. 2023

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“…The synthesis of fungal enzymes is the rst and most important step in enzyme technology, as it determines the process's economic viability. A considerable number of cellulases have been produced from fungal species such as Aspergillus or nature [8], Penicillium sp [9], Aspergillus terries MS [10], Aspergillus terries MI [11,12], Aspergillus niger and Rhizopes [13,14], Trichoderma viride [13], and Trichoderma longibrachiatum [15].…”

Section: Introductionmentioning

confidence: 99%

Bioprocess development for Extraction and purification of cellulases from Aspergillus niger 3ASZ using statistical experimental design techniques

Sorour

Olama

El-Naggar

et al. 2022

Preprint

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Background: The amount of cellulosic materials is large and may lead to environmental pollution, so it can be converted into useful materials for use in food or energy. Statistical design (Plackett–Burman and Box-Behnken) was the main topic of this study and was used to optimize the effect of environmental factors on cellulase production by Aspergillus niger. Results: Cellulase production using Plackett–Burman was 6.86-fold higher than the production of cellulase using basal medium. Subsequently, the design of BOX-Benken was affected to find the superlative procedure environments. The expected cellulase activity was 79.4 U/ml/min, which was adjacent to 18 times the enzyme activity. Ammonium sulfate precipitation was applied to the crude enzyme, followed by sequential fractionation with an Amicon system. The Amicon was used to demonstrate the final volume, total enzyme activity, specific activity, purification fold, and yield of cellulase (partially purified enzyme). Conclusion: Numerous cellulolytic enzymes are abundant in Aspergillusspecies. All of the data showed that Aspergillus sp. might be a reliable source of industrially and economically useful cellulases. By statistically calculating the relevance of a large number of elements in one experiment using a multifactorial statistical design, time may be saved while still maintaining the validity of each component.

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“…As the most abundant carbohydrate in nature, cellulose is an essential class of renewable resources (Li et al 2021). Its biotransformation degradation technology has become a research hotspot for both energy and the environmental sciences because of its high degradation efficiency, low energy consumption, safety, and lack of pollution (Awais et al 2021). However, little research has been reported on the screening and performance of low-temperature resistant cellulose-degrading strains.…”

Section: Introductionmentioning

confidence: 99%

Screening and characterization of a low-temperature-resistant cellulose-degrading strain, Trichoderma harzianum L-8, from a primitive forest

Wang

Tao

et al. 2022

BioRes

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Low temperature is a major factor limiting the bio-sustainable and efficient conversion of cellulose-based resources in cold regions. In this study, a low-temperature resistant cellulose-degrading fungus with high cellulase production was screened from samples found in a primitive forest in Daqing by straw using the enrichment-restricted culture technique. The fungus was identified as genus Trichoderma harzianum, strain L-8 by morphological and molecular biological analysis. The enzyme production conditions were optimized via response surface methodology, and the optimal conditions for the enzyme production of Trichoderma harzianum L-8 were as follows: a CMC-Na addition of 10.63 g·L-1, an ammonium sulfate addition of 2.22 g·L-1, an initial pH of 5.29, and a lecithin addition of 5.18 g·L-1 when the CMCase reached 53.40 IU·mL-1. The leading enzyme families of Trichoderma harzianum L-8 were identified via proteomic analysis. Proteases including glycosyl hydrolase family 3-4 and cellobiohydrolase play important roles in cellulose degradation. The strain Trichoderma harzianum L-8 showed a strong cellulose degradation ability under low temperatures, providing strain resources for cellulose resource biotransformation technology in cold regions.

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Enhanced biodegradation of organic waste treated by environmental fungal isolates with higher cellulolytic potential

Cited by 3 publications

References 61 publications

Application of a novel biological-nanoparticle pretreatment to Oscillatoria acuminata biomass and coculture dark fermentation for improving hydrogen production

Application of a novel biological-nanoparticle pretreatment to Oscillatoria acuminata biomass and coculture dark fermentation for improving hydrogen production

Bioprocess development for Extraction and purification of cellulases from Aspergillus niger 3ASZ using statistical experimental design techniques

Screening and characterization of a low-temperature-resistant cellulose-degrading strain, Trichoderma harzianum L-8, from a primitive forest

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