Bioprocess development for Extraction and purification of cellulases from Aspergillus niger 3ASZ using statistical experimental design techniques

Abstract

Background: The amount of cellulosic materials is large and may lead to environmental pollution, so it can be converted into useful materials for use in food or energy. Statistical design (Plackett–Burman and Box-Behnken) was the main topic of this study and was used to optimize the effect of environmental factors on cellulase production by Aspergillus niger. Results: Cellulase production using Plackett–Burman was 6.86-fold higher than the production of cellulase using basal medium. Subsequently, the design of BOX-Benken was affected to find the superlative procedure environments. The expected cellulase activity was 79.4 U/ml/min, which was adjacent to 18 times the enzyme activity. Ammonium sulfate precipitation was applied to the crude enzyme, followed by sequential fractionation with an Amicon system. The Amicon was used to demonstrate the final volume, total enzyme activity, specific activity, purification fold, and yield of cellulase (partially purified enzyme). Conclusion: Numerous cellulolytic enzymes are abundant in Aspergillusspecies. All of the data showed that Aspergillus sp. might be a reliable source of industrially and economically useful cellulases. By statistically calculating the relevance of a large number of elements in one experiment using a multifactorial statistical design, time may be saved while still maintaining the validity of each component.