Enhanced biodegradation and valorization of drilling wastewater via simultaneous production of biosurfactants and polyhydroxyalkanoates by Pseudomonas citronellolis SJTE-3

Koutinas, Michalis; Kyriakou, Maria; Andreou, Kostas; Hadjicharalambous, Michalis; Kaliviotis, Efstathios; Pasias, Dimitris; Kazamias, George; Varavvas, Costas; Vyrides, Ioannis

doi:10.1016/j.biortech.2021.125679

Cited by 12 publications

(2 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sequencing alignment was performed by Macrogene (Netherlands) and the resulting alignment of the DNA sequences of the gene encoding 16S rRNA was compared for homology in the NCBI database by BLASTn nucleotide tool analysis. The phylogenetic tree diagram was developed through the neighbor-joining method applying Mega 6.0 software and the most similar sequences according to the NCBI database by BLASTn and boot strapping similar to Koutinas et al [30].…”

Section: Strain Characterization By 16s Rrna Sequence Analysismentioning

confidence: 99%

Assessment of Bilge Water Degradation by Isolated Citrobacter sp. and Two Indigenous Strains and Identification of Organic Content by GC-MS

Gatidou

Drakou

Vyrides

2022

Water

Self Cite

View full text Add to dashboard Cite

Bilge water is oily saline wastewater accumulated on the hull at the bottom of a vessel, generated from leakage from pipes and engines and wash-down freshwater containing cleaning solvents. The present study focused on isolating microorganisms from oil-contaminated sites and indigenous species from raw bilge water and assessment of their ability to biodegrade bilge water. Using phenanthrene as a carbon source Citrobacter species was isolated from oil-contaminated sites and its optimum growth condition was found. The results indicated significant tolerance of the bacterium which presented great biodegradation ability for the tested carbon source. At high salinity (33 g L−1 of NaCl), sufficient phenathrene removal was achieved (81%), whereas variation of pH from 5 to 10 did not affected the survival of the microorganism. Regarding the effect of temperature and nutrients, Citrobacter sp. was better adapted at 30 °C, while lack of nutrients presented a negative impact on its growth. Halomonas and Exiguobacterium sp. were isolated from real bilge water using phenanthrene and phenol as a carbon source. The isolated strains independently exposed to high and low range bilge water pointed out around 83% and 53% chemical oxygen demand (COD) removal, respectively. Analysis of untreated bilge water by gas chromatography-mass spectrometry (GC-MS) was carried out, and the results confirmed the presence of organic compounds having a high similarity with Heptane, N-hexadecanoic acid, Methyl isobutyl Ketone and 1-butoxy-2-propanol. Chromatographic analysis of treated bilge water after exposure to isolated strains indicated the existence of new compounds. These metabolites presented high similarity with N-hexadecanoic, methyl ester, N-hexadecanoic and Octadecanoic acid methyl ester.

show abstract

Section: Strain Characterization By 16s Rrna Sequence Analysismentioning

confidence: 99%

Assessment of Bilge Water Degradation by Isolated Citrobacter sp. and Two Indigenous Strains and Identification of Organic Content by GC-MS

Gatidou

Drakou

Vyrides

2022

Water

Self Cite

View full text Add to dashboard Cite

show abstract

“…Non-pathogenic Pseudomonas species such as P. putida, P. citronellolis, P. cepacian, and other Pseudomonas spp. are less mentioned as potential rhamnolipid producers in the scientific literature [35][36][37][38][39]. Mono-rhamnolipid synthesis in bacteria involves the rhamnotransferase RhlB, while dTDP-L-rhamnose and β-hydroxyl fatty acids are used as precursors.…”

Section: Introductionmentioning

confidence: 99%

Di- and Mono-Rhamnolipids Produced by the Pseudomonas putida PP021 Isolate Significantly Enhance the Degree of Recovery of Heavy Oil from the Romashkino Oil Field (Tatarstan, Russia)

et al. 2022

View full text Add to dashboard Cite

Around the globe, only 30–50% of the amount of oil estimated to be in reservoirs (“original oil in place”) can be obtained using primary and secondary oil recovery methods. Enhanced oil recovery methods are required in the oil processing industry, and the use of microbially produced amphiphilic molecules (biosurfactants) is considered a promising efficient and environmentally friendly method. In the present study, biosurfactants produced by the Pseudomonas putida PP021 isolate were extracted and characterized, and their potential to enhance oil recovery was demonstrated. It was found that the cell-free biosurfactant-containing supernatant decreased the air–water interface tension from 74 to 28 mN m−1. Using TLC and FTIR methods, the biosurfactants produced by the isolate were classified as mono- and di-rhamnolipid mixtures. In the isolates’ genome, the genes rhlB and rhlC, encoding enzymes involved in the synthesis of mono- and di-rhamnolipids, respectively, were revealed. Both genes were expressed when the strain was cultivated on glycerol nitrate medium. As follows from the sand-packed column and core flooding simulations, biosurfactants produced by P. putida PP021 significantly enhance the degree of recovery, resulting in additional 27% and 21%, respectively.

show abstract

Co‐production of Biosurfactants and Other Bioproducts in Biorefineries

Vélez‐Mercado,

Espinosa‐Lavenant,

Flores‐Iga

et al. 2023

Biosurfactants and Sustainability

View full text Add to dashboard Cite

Enhanced biodegradation and valorization of drilling wastewater via simultaneous production of biosurfactants and polyhydroxyalkanoates by Pseudomonas citronellolis SJTE-3

Cited by 12 publications

References 47 publications

Assessment of Bilge Water Degradation by Isolated Citrobacter sp. and Two Indigenous Strains and Identification of Organic Content by GC-MS

Assessment of Bilge Water Degradation by Isolated Citrobacter sp. and Two Indigenous Strains and Identification of Organic Content by GC-MS

Di- and Mono-Rhamnolipids Produced by the Pseudomonas putida PP021 Isolate Significantly Enhance the Degree of Recovery of Heavy Oil from the Romashkino Oil Field (Tatarstan, Russia)

Co‐production of Biosurfactants and Other Bioproducts in Biorefineries

Contact Info

Product

Resources

About