Enhanced biodegradation and detoxification of malachite green by Trichoderma asperellum laccase: Degradation pathway and product analysis

Shanmugam, Sabarathinam; Ulaganathan, Priyadharshini; Swaminathan, K.; Sadhasivam, S.; Wu, Yi-Rui

doi:10.1016/j.ibiod.2017.08.001

Cited by 64 publications

(25 citation statements)

References 54 publications

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“…Laccase have great degradation potential for many aromatic compounds (Bhatia et al, 2017). Shanmugam et al (2017), have reported maximum biodegradation of Malachite Green by Trichoderma asperellum laccase activity which converted benzaldehyde from Malachite Green via the Michler's ketone pathway. Immobilization of laccase on Glutaraldehyde-crosslinked Chitosan Beads (GA-CBs) has been reported by Nguyen et al 2016, provided reusability and high catalytic ability which helped in degradation of sulfur dyes when concentration of laccase was low.…”

Section: Biological Degradationmentioning

confidence: 99%

Microbial degradation of dyes: An overview

Varjani

Rakholiya

et al. 2020

Bioresource Technology

379

109

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Industrialization increases use of dyes due to its high demand in paper, cosmetic, textile, leather and food industries. This in turn would increase wastewater generation from dye industrial activities. Various dyes and its structural compounds present in dye industrial wastewater have harmful effects on plants, animals and humans. Synthetic dyes are more resistant than natural dyes to physical and chemical methods for remediation which makes them more difficult to get decolorize. Microbial degradation has been researched and reviewed largely for quicker dye degradation. Genetically engineered microorganisms (GEMs) play important role in achieving complete dye degradation. This paper provides scientific and technical information about dyes & dye intermediates and biodegradation of azo dye. It also compiles information about factors affecting dye(s) biodegradation, role of genetically modified organisms (GMOs) in process of dye(s) degradation and perspectives in this field of research.

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Section: Biological Degradationmentioning

confidence: 99%

Microbial degradation of dyes: An overview

Varjani

Rakholiya

et al. 2020

Bioresource Technology

379

109

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“…The experiments were performed in triplicate, and data are shown as averages ± standard deviation. d Photographic record of dyes before and after treatment with purified TtL under optimized conditions described by Rezaei et al (2015), Daâssi et al (2013) and Shanmugam et al (2017).…”

Section: Mtl and Ttl Serve As Non-toxic Effective Catalysts For Decolorization Of Synthetic Textile Dyesmentioning

confidence: 99%

Decolorization and detoxification of textile wastewaters by recombinant Myceliophthora thermophila and Trametes trogii laccases

2018

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Laccases are multi-copper oxidoreductases with broad biotechnological applications. Here, we report detailed biochemical characterization of purified recombinant laccases originating from Myceliophthora thermophila (MtL) and Trametes trogii (TtL). We identified optimal conditions for decolorization of commercial dyes and textile wastewater samples. We also tested the toxicity of decolorized wastewater samples using human peripheral blood mononuclear cells. MtL and TtL were expressed in Saccharomyces cerevisiae, and secreted enzymes were purified by consecutive hydrophobic and gel chromatography. The molecular masses of TtL (~ 65 kDa) and MtL (> 100 kDa) suggested glycosylation of the recombinant enzymes. Deglycosylation of MtL and TtL led to 25% and 10% decreases in activity, respectively. In a thermal stability assay, TtL retained 61% and MtL 86% of the initial activity at 40 °C. While TtL retained roughly 50% activity at 60 °C, MtL lost stability at temperatures higher than 40 °C. MtL and TtL preferred syringaldazine as a substrate, and the catalytic efficiencies for ABTS oxidation were 7.5 times lower than for syringaldazine oxidation. In the presence of the mediator HBT, purified TtL almost completely decolorized dyes within 30 min and substantially decolorized wastewater samples from a textile factory (up to 74%) within 20 h. However, products of TtL-catalyzed decolorization were more toxic than MtL-decolorized products, which were almost completely detoxified.

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“…Biological methods are environmentally friendly and convert organic pollutants into less toxic compounds. MG can be degraded using fungal species, such as Saccharomyces cerevisiae [7], Cunninghamella elegans [8], and Trichoderma asperellum [9]. Fungal species exhibit long growth cycles and moderate decolorization rates, and are non-specific for target contaminants [10].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Malachite Green in Milkfish Pond Sediments

et al. 2019

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Malachite green (MG) is usually applied as a biocide in aquaculture worldwide. The microbial degradation of MG and changes in the microbial community composition of milkfish (Chanos chanos) culture pond sediments were assessed in this study. Three MG-degrading bacteria strains—M6, M10, and M12—were isolated, identified, and characterized. Strains M6, M10, and M12 are closely related to Zhouia amylolytica, Tenacibaculum mesophilum, and Enterobacter cloacae, respectively. The bacterial strains M10 and M12 showed good ability to degrade MG in the sediment. The MG degradation rate was increased after adding MG three more times. The microbial community in the sediment changes with different treatments. The bacterial strains M10 and M12 provide a potential solution for the treatment of sediment of saline aquaculture ponds with MG contamination.

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Enhanced biodegradation and detoxification of malachite green by Trichoderma asperellum laccase: Degradation pathway and product analysis

Cited by 64 publications

References 54 publications

Microbial degradation of dyes: An overview

Microbial degradation of dyes: An overview

Decolorization and detoxification of textile wastewaters by recombinant Myceliophthora thermophila and Trametes trogii laccases

Biodegradation of Malachite Green in Milkfish Pond Sediments

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