Enhanced Biobutanol Production in Folic Acid-Induced Medium by Using <i>Clostridium acetobutylicum</i> NRRL B-527

Nimbalkar, Pranhita R.; Khedkar, Manisha A.; Chavan, Prakash V.; Bankar, Sandip B.

doi:10.1021/acsomega.9b00583

Cited by 9 publications

(6 citation statements)

References 32 publications

(67 reference statements)

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“…Acids, various metals and minerals, vitamins, and amino acids also affect both solvent production and sporulation in clostridia (List et al 2019;Mukherjee et al 2019;Nimbalkar et al 2018Nimbalkar et al , 2019Reeve and Reid 2016), but few studies mentioned their impact on sporulation. Long et al (1984a) investigated butyrate and acetate's effect on sporulation in C. saccharobutylicum by adding them to the medium at the start of the fermentation in different concentrations.…”

Section: Other Media Componentsmentioning

confidence: 99%

Sporulation in solventogenic and acetogenic clostridia

Diallo

Kengen

López-Contreras

2021

Appl Microbiol Biotechnol

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The Clostridium genus harbors compelling organisms for biotechnological production processes; while acetogenic clostridia can fix C1-compounds to produce acetate and ethanol, solventogenic clostridia can utilize a wide range of carbon sources to produce commercially valuable carboxylic acids, alcohols, and ketones by fermentation. Despite their potential, the conversion by these bacteria of carbohydrates or C1 compounds to alcohols is not cost-effective enough to result in economically viable processes. Engineering solventogenic clostridia by impairing sporulation is one of the investigated approaches to improve solvent productivity. Sporulation is a cell differentiation process triggered in bacteria in response to exposure to environmental stressors. The generated spores are metabolically inactive but resistant to harsh conditions (UV, chemicals, heat, oxygen). In Firmicutes, sporulation has been mainly studied in bacilli and pathogenic clostridia, and our knowledge of sporulation in solvent-producing or acetogenic clostridia is limited. Still, sporulation is an integral part of the cellular physiology of clostridia; thus, understanding the regulation of sporulation and its connection to solvent production may give clues to improve the performance of solventogenic clostridia. This review aims to provide an overview of the triggers, characteristics, and regulatory mechanism of sporulation in solventogenic clostridia. Those are further compared to the current knowledge on sporulation in the industrially relevant acetogenic clostridia. Finally, the potential applications of spores for process improvement are discussed.Key Points• The regulatory network governing sporulation initiation varies in solventogenic clostridia.• Media composition and cell density are the main triggers of sporulation.• Spores can be used to improve the fermentation process.

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Section: Other Media Componentsmentioning

confidence: 99%

Sporulation in solventogenic and acetogenic clostridia

Diallo

Kengen

López-Contreras

2021

Appl Microbiol Biotechnol

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“…It has been reported that the supplementation of micronutrients/cofactors at low concentrations could maintain high ORP (oxidoreduction potential) levels, which is directly attributed to the earlier initiation of solventogenesis with improved butanol titer . Moreover, several research studies also pointed out that a higher concentration of metabolic precursors is detrimental to clostridial butanol production. , Hence, soy meal addition was not continued beyond 5 g L –1 in the present study. Nevertheless, around a threefold increment in butanol titer was achieved as compared to control (without soy meal).…”

Section: Resultsmentioning

confidence: 78%

“…40 Moreover, several research studies also pointed out that a higher concentration of metabolic precursors is detrimental to clostridial butanol production. 41,42 Hence, soy meal addition was not continued beyond 5 g L −1 in the present study. Nevertheless, around a threefold increment in butanol titer was achieved as compared to control (without soy meal).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Efficient Strategy to Alleviate the Inhibitory Effect of Lignin-Derived Compounds for Enhanced Butanol Production

Survase

Nimbalkar

Jurgens

et al. 2020

ACS Sustainable Chem. Eng.

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In the present study, the effect of one of the most important lignin-derived inhibitors (lignosulfonate) was assessed. A technique to overcome the lignosulfonate inhibitory action in the acetone-butanol-ethanol (ABE) fermentation process is proposed here. Different lignosulfonates were primarily added in the fermentation medium to observe their mechanistic action on the ABE production profile. Augmenting lignosulfonate concentration (>0.5 g L −1 ) resulted in a drastically reduced solvent titer (ABE ∼1.50 g L −1 ). Especially, low-molecularweight lignosulfonate (>1 g L −1 ) severely affected the solvent production and completely ceased the fermentation process. Therefore, a strategic approach that triggers the key genes responsible for butanol production was explored. The experimental analysis revealed that soy meal addition could enhance Clostridium acetobutylicum survival in the presence of lignosulfonates (0.25-3 g L −1 ). Moreover, soy meal addition also enhanced butanol concentration over 1.5-fold as compared to the control experiment. The ABE production using wood hydrolysate also produced substantial solvent titer (ABE ∼11 g L −1 ) in the presence of soy meal (5 g L −1 ). The transcriptional analysis results showed that important genes in clostridial metabolic pathways were up-regulated in the presence of soy meal addition during fermentation.

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“…It is produced as a “natural” fuel via the fermentation of potatoes, sugar cane, sugar beet, corn, cassava, sorghum, wheat and cellulose, with the use of the bacterium Clostridia acetobutilicum [ 78 ]. Applying Escherichia coli as a fermentation agent is much less effective in terms of the yield of biobutanol [ 79 ]. Another advantage over bioethanol is the ability of Clostridia acetobutilicum to transform biomass that contains polymeric cellulose [ 80 ].…”

Section: Conditionally Renewable Alternative Sources Of Energymentioning

confidence: 99%

Using Alternative Sources of Energy for Decarbonization: A Piece of Cake, but How to Cook This Cake?

Boguslavsky

Sharov

Шарова

2022

IJERPH

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Few analytical or research works claim that the negative impact of improper use of ASEs may be comparable with that of hydrocarbons and sometimes even greater. It has become a common view that “green” energy (ASE) is clean, safe and environmentally friendly (eco-friendly) in contrast with “black” energy (hydrocarbons). We analyzed 144 works on systemic and/or comparative research of the modern and prospective ASE: biofuels, hydrogen, hydropower, nuclear power, wind power, solar power, geothermal power, oceanic thermal power, tidal power, wind wave power and nuclear fusion power. We performed our analysis within the Spaceship Earth paradigm. We conclude that there is no perfect ASE that is always eco-friendly. All ASEs may be dangerous to the planet considered as a closed and isolated unit (“spaceship”) if they are used in an inconsistent manner. This is not in the least a reason to deny them as prospective sources of energy. Using all ASEs in different proportions in various regions of the planet, where their harm to the planet and humanity can be minimized and, on the contrary, their efficiency maximized, would give humanity the opportunity to decarbonize the Earth, and make the energy transition in the most effective way.

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Enhanced Biobutanol Production in Folic Acid-Induced Medium by Using Clostridium acetobutylicum NRRL B-527

Cited by 9 publications

References 32 publications

Sporulation in solventogenic and acetogenic clostridia

Sporulation in solventogenic and acetogenic clostridia

Efficient Strategy to Alleviate the Inhibitory Effect of Lignin-Derived Compounds for Enhanced Butanol Production

Using Alternative Sources of Energy for Decarbonization: A Piece of Cake, but How to Cook This Cake?

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