Enhanced Aortic Nerve Growth Factor Expression and Nerve Sprouting in Rats Following Gastric Perforation

Tsai, Ming-Shian; Ko, Ya-Hui; Hsu, Wen Ming; Liang, Jin–Tung; Lai, Hong‐Shiee; Lee, Po‐Huang; Chang, Kuo‐Chu

doi:10.1016/j.jss.2010.01.021

Cited by 4 publications

(6 citation statements)

References 37 publications

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“…The present model has some limitations. The experimental sutured gastric perforation is slightly different from the perforation caused by human gastric ulcer, although this model has been reported previously. Generally, perforated ulcer causes severe abdominal inflammation that adversely influences healing, especially when the peritoneal cavity is contaminated by gastrointestinal content.…”

Section: Discussionmentioning

confidence: 86%

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Effect of local injection of mesenchymal stem cells on healing of sutured gastric perforation in an experimental model

Liu

Chiu

Lam

et al. 2015

British Journal of Surgery

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Local injection of mesenchymal stem cells was more effective than topical application, and enhanced the healing of sutured gastric perforations by an anti-inflammatory process, enhanced cellular proliferation and earlier onset of granulation. Surgical relevance Abnormal healing of gastric perforation may cause morbidity and increase the risk of death. Adipose tissue-derived mesenchymal stem cells have been found to promote the healing of organ injuries through cellular differentiation and secretion of cytokines that stimulate cellular proliferation and angiogenesis, and suppress inflammation. This study explored the therapeutic potential of such mesenchymal stem cells for promotion of the healing of sutured gastric perforations. Mesenchymal stem cells delivered by local injection significantly enhanced the healing of gastric perforations with reduced severity of wound adhesion, and a decreased incidence of wound dehiscence and abdominal abscess. The increased expression of transforming growth factor β1, proliferating cell nuclear antigen and reduced level of interleukin 6 provide evidence for enhancement of the healing process. Engrafted mesenchymal stem cells expressed α-smooth muscle actin as a marker of myofibroblasts. This preclinical study indicates that local injection of allogeneic adipose tissue-derived mesenchymal stem cells may have a potential therapeutic role in enhancing the healing of peptic ulcer disease and prevention of ulcer-related complications.

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Section: Discussionmentioning

confidence: 86%

“…. Briefly, sutured gastric perforation in rats was performed under sterile conditions, as described previously. Female wild‐type Sprague‐Dawley rats weighing between 200 and 250 g were used.…”

Section: Methodsmentioning

confidence: 99%

Effect of local injection of mesenchymal stem cells on healing of sutured gastric perforation in an experimental model

Liu

Chiu

Lam

et al. 2015

British Journal of Surgery

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“…Since NGF has been previously reported to be up-regulated by inflammatory cytokines in diseased bladder [6] , aorta [23] , heart [11] , and livers [17] , [18] , [24] , [25] , we next to determine whether anti-inflammatory treatment could ameliorate the cholestasis-associated NGF up-regulation in injured livers. A synthetic glucocorticoid drug (MP) was used to suppress systemic immunactivity and thereby clarify the causal relationship between inflammation and systemic and/or hepatic NGF up-regulation.…”

Section: Resultsmentioning

confidence: 99%

Up-Regulation of Nerve Growth Factor in Cholestatic Livers and Its Hepatoprotective Role against Oxidative Stress

et al. 2014

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The role of nerve growth factor (NGF) in liver injury induced by bile duct ligation (BDL) remains elusive. This study aimed to investigate the relationship between inflammation and hepatic NGF expression, to explore the possible upstream molecules up-regulating NGF, and to determine whether NGF could protect hepatocytes from oxidative liver injury. Biochemical and molecular detection showed that NGF was up-regulated in cholestatic livers and plasma, and well correlated with systemic and hepatic inflammation. Conversely, systemic immunosuppression reduced serum NGF levels and resulted in higher mortality in BDL-treated mice. Immunohistochemistry showed that the up-regulated NGF was mainly localized in parenchymal hepatocytes. In vitro mechanistic study further demonstrated that TGF-β1 up-regulated NGF expression in clone-9 and primary rat hepatocytes. Exogenous NGF supplementation and endogenous NGF overexpression effectively protected hepatocytes against TGF-β1- and oxidative stress-induced cell death in vitro, along with reduced formation of oxidative adducted proteins modified by 4-HNE and 8-OHdG. TUNEL staining confirmed the involvement of anti-apoptosis in the NGF-exhibited hepatoprotection. Moreover, NGF potently induced Akt phosphorylation and increased Bcl-2 to Bax ratios, whereas these molecular alterations by NGF were only seen in the H2O2-, but not TGF-β1-treated hepatocytes. In conclusion, NGF exhibits anti-oxidative and hepatoprotective effects and is suggested to be therapeutically applicable in treating cholestatic liver diseases.

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“…These were the sham group (Control) and two study groups, 1 h (G1) and 2 h (G2). In G1 and G2 all measurements were made at 1 h and 2 h respectively following acute peritonitis was induced by gastric perforation (GP) (16,17). All rats were weighed and anesthetized via an intraperitoneal injection of pentobarbital sodium (50 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

“…This method has been validated against the gold standard of SR vesicle preparations (16). The incubation buffer comprised 100 mM KCl, 20 mM HEPES, 7.5 mM pyrophosphate and 0.5 mM Mg 2+ (pH 7.0, 37°C).…”

Section: Methodsmentioning

confidence: 99%

Effect of acute peritonitis on rocuronium-induced intraperitoneal pressure reduction and the uptake function of the sarcoplasmic reticulum

Zhang

Gong

Yang

et al. 2017

Experimental and Therapeutic Medicine

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Previous studies have reported the incomplete relaxation effect of neuromuscular blockers on skeletal muscles in acute peritonitis (AP) and other inflammatory processes; however, the underlying mechanisms responsible for this effect have not yet been satisfactorily identified. The impaired removal of cytosolic Ca2+ through sarcoendoplasmic Ca2+-ATPase (SERCA) and defects in sarcoplasmic reticulum (SR) Ca2+ uptake are the major contributing factors to diastolic dysfunction. Previous studies on the effects of neuromuscular blockers have primarily focused on neuromuscular transmission. Because of the reduced calcium uptake in the SR itself, even when neuromuscular transmission is fully blocked, the muscle is not able to relax effectively. In the present study, the impact of AP on rocuronium-induced intraperitoneal pressure reduction and rectus abdominal muscle relaxation, and SERCA uptake function was investigated. AP was induced via gastric perforation and changes in the intraperitoneal pressure before and after the administration of rocuronium were recorded. Muscle contractile properties, uptake and release functions and SERCA activity in the rectus abdominal muscles of AP model rats were measured. The half-relaxation time in the AP group was significantly prolonged compared with that in the control group (P<0.01). The peak rate of SR Ca2+ uptake for whole muscle homogenates was significantly reduced (P<0.05) in AP model rats without reduction of the rate of Ca2+ release evoked through AgNO3. In conclusion, gastric perforation-induced AP attenuates the intraperitoneal pressure-reducing effect of rocuronium, and AP induces diastolic dysfunction of the rectus abdominal muscle. The SR Ca2+-ATPase uptake rate was also reduced by AP.

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Enhanced Aortic Nerve Growth Factor Expression and Nerve Sprouting in Rats Following Gastric Perforation

Cited by 4 publications

References 37 publications

Effect of local injection of mesenchymal stem cells on healing of sutured gastric perforation in an experimental model

Effect of local injection of mesenchymal stem cells on healing of sutured gastric perforation in an experimental model

Up-Regulation of Nerve Growth Factor in Cholestatic Livers and Its Hepatoprotective Role against Oxidative Stress

Effect of acute peritonitis on rocuronium-induced intraperitoneal pressure reduction and the uptake function of the sarcoplasmic reticulum

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