Enhanced Antipsoriatic Activity of Mycophenolic Acid Against the TNF-α-Induced HaCaT Cell Proliferation by Conjugated Poloxamer Micelles

Supasena, Wiwat; Muangnoi, Chawanphat; Thaweesest, Wuttinont; Songkram, Chalermkiat; Ueda, Keisuke; Higashi, Kenjirou; Moribe, Kunikazu; Tanasupawat, Somboon; Rojsitthisak, Pornchai

doi:10.1016/j.xphs.2019.11.010

Cited by 13 publications

(15 citation statements)

References 18 publications

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“…In the TNF-α induced hyperproliferation, TNF-α at 10 ng/mL was suitable for inducing the psoriasis condition by a significant increase in HaCaT cell proliferation by 53% ( p < 0.001) compared to the control group. This is consistent with our previous report [ 13 ]. Then, the antiproliferative effects of the BF from each compound were determined and the results presented as %cell proliferation were shown in Figure 5 .…”

Section: Resultssupporting

confidence: 95%

“…The synthesized MPA-Cur conjugate was structurally elucidated by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-IR), 1 H-, and 13 C-nuclear magnetic resonance spectroscopy (NMR), and high-resolution mass spectrometry (MS). The IR sample was dispersed on the diamond crystal pan.…”

Section: Structural Elucidation Of Mpa-curmentioning

confidence: 99%

“…The following are available online at https://www.mdpi.com/article/10 .3390/pharmaceutics13070956/s1, Figure S1: IR spectrum of MPA, CUR, and MPA-CUR conjugate, Figure S2: 1 H-NMR spectrum of MPA, CUR, and MPA-CUR conjugate, Figure S3: 13 C-NMR spectrum of MPA, CUR, and MPA-CUR conjugate, Figure S4. MS spectrum of MPA-CUR.…”

Section: Supplementary Materialsmentioning

confidence: 99%

“…MPA works through a mechanism of inhibition of the inosine monophosphate dehydrogenase (IMPDH) enzyme, which is crucial for purine synthesis. Inhibition of IMPDH activity reduces guanosine triphosphate (GTP) and d-guanosine triphosphate (dGTP) levels and drawbacks cell proliferation [ 13 ]. In addition, as an anti-inflammatory agent, MPA has demonstrated its ability to reduce the release of pro-inflammatory cytokines such as TNF-α and IL-1β in different cell lines [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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A Novel Curcumin-Mycophenolic Acid Conjugate Inhibited Hyperproliferation of Tumor Necrosis Factor-Alpha-Induced Human Keratinocyte Cells

et al. 2021

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Curcumin (CUR) has been used as adjuvant therapy for therapeutic application in the treatment of psoriasis through several mechanisms of action. Due to the poor oral bioavailability of CUR, several approaches have been developed to overcome the limitations of CUR, including the prodrug strategy. In this study, CUR was esterified with mycophenolic acid (MPA) as a novel conjugate prodrug. The MPA-CUR conjugate was structurally elucidated using FT-IR, 1H-NMR, 13C-NMR, and MS techniques. Bioavailable fractions (BFs) across Caco-2 cells of CUR, MPA, and MPA-CUR were collected for further biological activity evaluation representing an in vitro cellular transport model for oral administration. The antipsoriatic effect of the BFs was determined using antiproliferation and anti-inflammation assays against hyperproliferation of tumor necrosis factor-alpha (TNF-α)-induced human keratinocytes (HaCaT). The BF of MPA-CUR provided better antiproliferation than that of CUR (p < 0.001). The enhanced hyperproliferation suppression of the BF of MPA-CUR resulted from the reduction of several inflammatory cytokines, including IL-6, IL-8, and IL-1β. The molecular mechanisms of anti-inflammatory activity were mediated by an attenuated signaling cascade of MAPKs protein, i.e., p38, ERK, and JNK. Our results present evidence for the MPA-CUR conjugate as a promising therapeutic agent for treating psoriasis by antiproliferative and anti-inflammatory actions.

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Section: Resultssupporting

confidence: 95%

Section: Structural Elucidation Of Mpa-curmentioning

confidence: 99%

Section: Supplementary Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Novel Curcumin-Mycophenolic Acid Conjugate Inhibited Hyperproliferation of Tumor Necrosis Factor-Alpha-Induced Human Keratinocyte Cells

et al. 2021

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“…Further skin diffusion studies have demonstrated that in terms of skin permeation, the amphoteric emulsifiers were superior to non-ionic surfactants, but inferior to anionic emulsifiers ( Karande et al, 2005 ; Pham et al, 2016 ). In addition, different patterns of drug release from micelles have been proposed to be dependent on the loading method and drug location; e.g., drugs loaded by physical entrapment or located in the shell/interface are released by diffusion, while drugs loaded by chemical conjugation or located in the center are released by micelle degradation or surface erosion ( Kosakowska et al, 2018 ; Supasena et al, 2020 ).…”

Section: Nanoparticlesmentioning

confidence: 99%

Transdermal Delivery of Therapeutic Compounds With Nanotechnological Approaches in Psoriasis

Qin

Dai

et al. 2022

Front. Bioeng. Biotechnol.

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Psoriasis is a chronic, immune-mediated skin disorder involving hyperproliferation of the keratinocytes in the epidermis. As complex as its pathophysiology, the optimal treatment for psoriasis remains unsatisfactorily addressed. Though systemic administration of biological agents has made an impressive stride in moderate-to-severe psoriasis, a considerable portion of psoriatic conditions were left unresolved, mainly due to adverse effects from systemic drug administration or insufficient drug delivery across a highly packed stratum corneum via topical therapies. Along with the advances in nanotechnologies, the incorporation of nanomaterials as topical drug carriers opens an obvious prospect for the development of antipsoriatic topicals. Hence, this review aims to distinguish the benefits and weaknesses of individual nanostructures when applied as topical antipsoriatics in preclinical psoriatic models. In view of specific features of each nanostructure, we propose that a proper combination of distinctive nanomaterials according to the physicochemical properties of loaded drugs and clinical features of psoriatic patients is becoming a promising option that potentially drives the translation of nanomaterials from bench to bedside with improved transdermal drug delivery and consequently therapeutic effects.

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An Overview of Natural and Synthetic Phthalides Involved in Cancer Studies: Past, Present, and Future

et al. 2022

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Phthalides, mainly isolated from two genera: Ligusticum and Angelica of the Apiceae (syn Umbelliferae) family, are thought to be a potent natural compound with many therapeutic effects, such as antiplatelet, antitussive, antinociceptive, anti‐inflammatory, antibacterial, antiviral, and immunosuppressive activities. They also have antifungal, antioxidant, and anticancer activities. Phthalides are responsible for the characteristic odor of celery. Since ancient times, phthalides containing plants have been used in Asia, Europe, and North America as traditional medicines and food flavoring agents. In this review, we have provided the data regarding the mechanisms of action related with phthalides on different cancer cells, their chemopreventive properties, and their possible therapeutic potential against various types of human cancer. Our attempt is to make a case for the use of phthalide and their derivatives as one of the plant‐based compounds in targeting metabolic treatment of cancer diseases.

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Enhanced Antipsoriatic Activity of Mycophenolic Acid Against the TNF-α-Induced HaCaT Cell Proliferation by Conjugated Poloxamer Micelles

Cited by 13 publications

References 18 publications

A Novel Curcumin-Mycophenolic Acid Conjugate Inhibited Hyperproliferation of Tumor Necrosis Factor-Alpha-Induced Human Keratinocyte Cells

A Novel Curcumin-Mycophenolic Acid Conjugate Inhibited Hyperproliferation of Tumor Necrosis Factor-Alpha-Induced Human Keratinocyte Cells

Transdermal Delivery of Therapeutic Compounds With Nanotechnological Approaches in Psoriasis

An Overview of Natural and Synthetic Phthalides Involved in Cancer Studies: Past, Present, and Future

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