Enhanced antioxidant effect oftrans-resveratrol: potential of binary systems with polyethylene glycol and cyclodextrin

Abstract

Trans-resveratrol, a polyphenol extracted from Vitis vinifera, has different beneficial effects following its administration on the skin. Here the potential use of binary systems to enhance in vitro and in vivo activity of trans-resveratrol was investigated. Thus the aqueous solubility of trans-resveratrol was investigated in the presence of growing concentrations of polyethylene glycol (PEG) or β-cyclodextrin (βCD) as solubilizing excipients. Then, the solid dispersion of trans-resveratrol with PEG or inclusion complexes trans-resveratrol/βCD were prepared and characterised by different methods. Cytotoxicity and inhibition of reactive oxygen species (ROS) following H2O2 challenge in the presence of trans-resveratrol, alone or associated to the excipients, was evaluated on human keratinocyte HaCaT cell line. Both the trans-resveratrol-containing binary systems induced significant reduction of H2O2-induced ROS production, especially in the case of βCD that was selected for the following phase of the study. Thus, the effect of a cream containing trans-resveratrol, alone or associated to βCD, on different skin parameters such as corneometry, colorimetry and elastometry, was evaluated on human volunteers. All patients showed a visible improvement of clinical conditions with a remarkable decrease of aging signs, but this effect was higher of the hemi face treated with the βCD-containing formulation versus formulation containing trans-resveratrol alone.

Polyphenols as active ingredients for cosmetic products

“…[103]. A visible improvement of ageing signs as elasticity was achieved by the application of a cream containing transresveratrol, especially in the combination with beta-cyclodextrin as carrier, probably due to enhancement of the skin permeation rate [104].…”

Review of the Pharmacological Effects ofVitis vinifera(Grape) and its Bioactive Constituents: An Update

“…Moreover, the use of resveratrol in combination with β-CD was more effective than resveratrol alone. However, the molecular mechanisms underlying this combined action are unclear (Moyano-Mendez et al, 2014).…”

Resveratrol supplementation: Where are we now and where should we go?

“…The presence of specific RSV receptor sites in human skin suggests that this polyphenolic compound may be useful to prevent skin disorders associated with aging (Bastianetto et al, 2010). The improvement of clinical signs of aging was even better when RSV was used in conjunction with β-cyclodextrin excipient (Moyano-Mendez et al, 2014). The anti-acneic property of RSV in volunteers with acne vulgaris has also been reported (Fabbrocini et al, 2011).…”