Enhanced anticancer potency of hydroxytyrosol and curcumin by <scp>PLGA‐PAA nano‐encapsulation</scp> on <scp>PANC</scp>‐1 pancreatic cancer cell line

Jadid, Mahdieh Farhoudi Sefidan; Shademan, Behrouz; Chavoshi, Reza; Seyyedsani, Nasrin; Aghaei, Elnaz; Taheri, Elham; Goleij, Pouya; Hajazimian, Saba; Karamad, Vahidreza; Behroozi, Javad; Sabet, Mehrdad Nasrollahzadeh; Isazadeh, Alireza; Baradaran, Behzad

doi:10.1002/tox.23103

Cited by 40 publications

(25 citation statements)

References 27 publications

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“…GBM is highly resistant to most treatments due to its heterogeneous nature and the presence of TMZ and RT-resistant cancer stem cells [17]. Numerous studies have shown the importance of using drugs in combination (simultaneous use of a combination of two drugs) to reduce the growth of cancer cells and lead cancer cells to apoptosis [25][26][27]. Therefore, we used KC7F2 to ameliorate the effects of TMZ.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of therapeutic effects of KC7F2, temozolomide, and its combination on genes involved in the Wnt signal pathway in the U87MG glioma cell line

Korkmaz¹,

Korkmaz²,

Bağca

et al. 2022

Preprint

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Glioma is the most common malignant primary brain tumor that survives less than 12 months after diagnosis. The Wnt signaling pathway creates a complex network of proteins that have different effects on cells, so they can be involved in many cancers, including gliomas. Drug resistance is seen in many cancer treatments. Therefore, we investigated the combined effect of Temozolomide (TMZ) and KC7F2 on cell survival and changes in gene expression in the Wnt signal pathway. The effects of TMZ and KC7F2 on cancer cell viability and proliferation were investigated by WST-1 method. Isobologram analysis was performed to evaluate the combined effects of TMZ and KC7F on U87MG glioma cell line. Also, Quantitative RT-PCR was used to evaluate the mRNA expression level of genes related to the Wnt signal pathway in treated cancer cells and control groups. IC50 TMZ and KC7F2 values in U87MG cell lines were determined to be 461µM and 19µM in 48 hours, respectively. Combined effects of TMZ and KC7F2 on the U87MG cell line show the CI values for TMZ and KC7F2 were found as 0.746. It was found that KC7F2+TMZ increased APC, CCND1, CXXC4, DAAM1, FBXW11, FRZB, FZD3, FZD8, LEF1, LRP5, LRP6, PYGO1, RHOA, RHOU, RUVBL1, VANGL2, WIF1, WNT10A, WNT16, WNT3A, and WNT6 while decreasing NKD1, PRICKLE1, DKK1, WNT1 expressions in U87MG cell lines. The results show that the combination of TMZ and KC7F2 can be a promising anti-cancer agent for the treatment of glioblastoma through the Wnt / β-catenin signaling pathway. However, further studies are needed to understand all angles.

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Section: Discussionmentioning

confidence: 99%

Evaluation of therapeutic effects of KC7F2, temozolomide, and its combination on genes involved in the Wnt signal pathway in the U87MG glioma cell line

Korkmaz¹,

Korkmaz²,

Bağca

et al. 2022

Preprint

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“…Natural and synthetic compounds are used as chemotherapeutic agents and are one of the treatment options in cancer treatment [17,18]. However, the toxic side effects of synthetic chemotherapeutic agents and the stringent approval procedures before administering them to patients have led researchers to focus on natural compounds to provide safer alternatives for cancer treatment and shorten the drug approval time [19].…”

Section: Discussionmentioning

confidence: 99%

Enhanced Anti-Cancer Potency Using A Combination of Oleanolic Acid and Maslinic Acid to Control Treatment Resistance in Breast Cancer.

Avcı

Söğütlü

Özateş

et al. 2021

Preprint

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The phosphatidylinositol 3-kinase/AKT/mammalian target of Rapamycin (PI3K/AKT/mTOR) pathway is a complex intracellular metabolic pathway leading to cell growth, and tumor proliferation plays an essential role in drug resistance in breast cancer. Therefore, in the present study, the anti-cancer effects of oleanolic acid (OA) and maslinic acid (MA) were investigated to improve the performance of the treatment strategy. We investigated the effect of OA and MA on cell viability using the WST-1 method. The synergistic effect of the combination was analyzed by isobologram analysis. In addition, the effects of the two compounds, individually and in combination, on apoptosis, autophagy, and the cell cycle were investigated in MCF7 cells. In addition, changes in the expression of PI3K/AKT/mTOR genes involved in apoptosis, cell cycle and metabolism were determined by quantitative RT-PCR. MA, OA, and a combination of both caused G0/G1 arrest. Apoptosis also increased in all treated groups. The autophagosomal LC3-II formation was induced 1.74-fold in the MA-treated group and 3.25-fold in the MA-OA-treated group. The combination treatment resulted in increased expression of genes such as GSK3B, PTEN, CDKN1B and FOXO3 and decreased expression of IGF1, PRKCB and AKT3 genes. The results showed that the combination of these two substances showed the highest synergistic effect at the lowest dose and using MA-OA caused cancer cells to undergo apoptosis. The use of combination drugs may reduce the resistance of cancer cells to treatment. However, further studies are needed to clarify cases.

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“…The anti-cancer activity of free and poly lactide-co-glycolide-co-polyacrylic acid nano-encapsulated CUR on PANC-1 cell lines was reported. This study revealed that free and nano-encapsulated CUR significantly increased apoptosis rates and decreased viability, migration and colony formation in the PANC-1 cell line with the nano-encapsulated CUR exerting a more profound apoptotic and anti-proliferative effect on the PANC-1 pancreatic cell line than the free CUR [ 124 ].…”

Section: Encapsulation and Evaluation Of Therapeutic Benefits Of Curmentioning

confidence: 99%

Enhancement of Biological and Pharmacological Properties of an Encapsulated Polyphenol: Curcumin

et al. 2021

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There is a dearth of natural remedies available for the treatment of an increasing number of diseases facing mankind. Natural products may provide an opportunity to produce formulations and therapeutic solutions to address this shortage. Curcumin (CUR), diferuloylmethane; I,7-bis-(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione is the major pigment in turmeric powder which has been reported to exhibit a number of health benefits including, antibacterial, antiviral, anti-cancer, anti-inflammatory and anti-oxidant properties. In this review, the authors attempt to highlight the biological and pharmacological properties of CUR in addition to emphasizing aspects relating to the biosynthesis, encapsulation and therapeutic effects of the compound. The information contained in this review was generated by considering published information in which evidence of enhanced biological and pharmacological properties of nano-encapsulated CUR was reported. CUR has contributed to a significant improvement in melanoma, breast, lung, gastro-intestinal, and genito-urinary cancer therapy. We highlight the impact of nano-encapsulated CUR for efficient inhibition of cell proliferation, even at low concentrations compared to the free CUR when considering anti-proliferation. Furthermore nano-encapsulated CUR exhibited bioactive properties, exerted cytotoxic and anti-oxidant effects by acting on endogenous and cholinergic anti-oxidant systems. CUR was reported to block Hepatitis C virus (HCV) entry into hepatic cells, inhibit MRSA proliferation, enhance wound healing and reduce bacterial load. Nano-encapsulated CUR has also shown bioactive properties when acting on antioxidant systems (endogenous and cholinergic). Future research is necessary and must focus on investigation of encapsulated CUR nano-particles in different models of human pathology.

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Enhanced anticancer potency of hydroxytyrosol and curcumin by PLGA‐PAA nano‐encapsulation on PANC‐1 pancreatic cancer cell line

Cited by 40 publications

References 27 publications

Evaluation of therapeutic effects of KC7F2, temozolomide, and its combination on genes involved in the Wnt signal pathway in the U87MG glioma cell line

Evaluation of therapeutic effects of KC7F2, temozolomide, and its combination on genes involved in the Wnt signal pathway in the U87MG glioma cell line

Enhanced Anti-Cancer Potency Using A Combination of Oleanolic Acid and Maslinic Acid to Control Treatment Resistance in Breast Cancer.

Enhancement of Biological and Pharmacological Properties of an Encapsulated Polyphenol: Curcumin

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